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Degradable material having multiple sensitive properties, manufacturing method thereof and use thereof

A multi-sensitivity, high-performance technology, used in recombinant DNA technology, introduction of foreign genetic material using vectors, etc.

Inactive Publication Date: 2010-04-21
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, gene carrier materials that are degradable and have triple and above sensitive properties have not been reported.

Method used

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  • Degradable material having multiple sensitive properties, manufacturing method thereof and use thereof
  • Degradable material having multiple sensitive properties, manufacturing method thereof and use thereof
  • Degradable material having multiple sensitive properties, manufacturing method thereof and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1: Preparation of the target product with a linear oligoethylenimine with a molecular weight of 423

[0042] According to the concentration of oligoethyleneimine in the organic solvent, the concentration of the substance is 0.2mol / L, and 4.23g of linear oligoethyleneimine with a molecular weight of 423 is dissolved in an equal volume mixed solvent of 50ml of chloroform and methanol, and then respectively according to The ratio of oligoethylenimine to N, N'-bisacryloylcystamine in Table 1, weigh N, N'-bisacryloylcystamine and add it into the reactor, mix well, and heat up to 45°C for reaction For 4 days, spin evaporate, concentrate the reaction solution, settle in 1000ml of n-hexane at 0°C, dry in vacuum, dissolve the obtained crude product in deionized water, and dialyze against deionized water in a dialysis bag with a molecular weight cut off of 3,500 for 4 days , filtered through a G4 sand core funnel, and freeze-dried to obtain the intermediate product cross-...

Embodiment 2

[0046] Embodiment 2: prepare target product with molecular weight being 600 hyperbranched oligoethylenimine

[0047] According to the amount concentration of oligopolyethyleneimine in the organic solvent, the concentration of the substance is 0.2mol / L, and 6g of hyperbranched oligopolyethyleneimine with a molecular weight of 600 is dissolved in an equal-volume mixed solvent of 50ml chloroform and methanol, and then respectively according to The ratio of oligoethylenimine to N,N'-bisacrylcystamine in Table 2, weigh N,N'-bisacrylcystamine and add it to the reactor, mix well, and heat up to 45°C for reaction For 4 days, spin evaporate, concentrate the reaction solution, settle in 1000ml of n-hexane at 0°C, and vacuum-dry. G4 sand core funnel was filtered and freeze-dried to obtain the intermediate product cross-linked oligoethylenimine. Weigh 0.5g of cross-linked oligoethylene imine and dissolve it in deionized water at 0.02mol / L, and then mix N - Add isopropylacrylamide into t...

Embodiment 3

[0051] Example 3: The target product was prepared with a hyperbranched oligoethylenimine with a molecular weight of 1800.

[0052] According to the amount concentration of oligopolyethyleneimine in the organic solvent, the concentration of the substance is 0.1mol / L, and 9g of hyperbranched oligopolyethyleneimine with a molecular weight of 1800 is dissolved in an equal-volume mixed solvent of 50ml chloroform and methanol, and then respectively according to The ratio of oligoethylenimine to N,N'-bisacryloylcystamine in Table 3, weigh N,N'-bisacryloylcystamine and add it to the reactor, mix well, and heat up to 45°C for reaction For 4 days, spin evaporate, concentrate the reaction solution, settle in 1000ml of n-hexane at 0°C, and vacuum-dry. G4 sand core funnel was filtered and freeze-dried to obtain the intermediate product cross-linked oligoethylenimine. Weigh 0.5g of cross-linked oligoethylene imine and dissolve it in deionized water at 0.02mol / L, and then mix N - Add isopr...

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Abstract

The invention relates to a degradable material having multiple sensitive properties, a manufacturing method thereof and use thereof. The manufacturing method comprises: dissolving oligo(polyethyleneimine) and N,N'-bis(acryloyl)cystamine in an organic solvent to allow the oligo(polyethyleneimine) and N,N'-bis(acryloyl)cystamine to cross link under a heating condition to form a network polymer; dissolving the network polymer and N-isopropyl acrylamide in deionized water; and heating mixture for reaction to form a target product. The cross-linked network polymer prepared by the method has multiple environmental pH, temperature and oxidoreduction sensitive properties. The visible light transmittance of the aqueous solution of the cross-linked network polymer gradually reduces to zero from 100 percent with the rise of temperature and the minimum critical consolute temperature of the aqueous solution of the cross-linked network polymer decreases with the increase of thepH value of the solution. In a reductive environment, with the breakage of disulfide bonds, the cross-linked network polymer degrades quickly into small molecular weight compounds, and the transmittance of the solution under visible light keeps constant as the temperature rises. The material can carry positive charges and is a novel multi-sensitivity polycation genonic vector that has small cytotoxicity and a wide application prospect.

Description

technical field [0001] The invention relates to a degradable material with multiple sensitive properties, a preparation method and an application, a network based on oligoethylenimine, N-isopropylacrylamide and N, N'-bisacryloylcystamine Cross-linked, degradable and multi-sensitive polymer with pH, ​​temperature and redox, its preparation method and its application in gene delivery. Background technique [0002] In recent years, the study of smart materials has aroused great interest of researchers (1-3). Smart materials with multiple responsiveness have always been a hot spot in this field. At present, more researches are on smart polymer materials with dual responsiveness, such as pH-temperature-sensitive polymer materials, temperature-redox-sensitive polymers, electric field- Magnetic field-sensitive polymers, etc., while the research on multiple sensitive materials with more than double responsiveness is less, the synthesis is generally more complicated, and most of the...

Claims

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Application Information

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IPC IPC(8): C08G73/02C12N15/85
Inventor 陈学思李非凡田华雨王献红景遐斌
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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