Gene for coding nitrilase of alcaligenes and method for preparing single enantiomorph of mandelic acid using same

A technology of nitrilase and mandelic acid, which is applied in the field of base sequences encoding nitrilase, can solve problems such as poor stability, low catalytic efficiency, and low enzyme yield, and achieve excellent results
CN101701222BInactive Publication Date: 2011-11-16EAST CHINA UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
EAST CHINA UNIV OF SCI & TECH
Publication Date
2011-11-16
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a base sequence for coding nitrilase of alcaligenes, relating to a carrier comprising the base sequence and a transformant comprising the base sequence or the carrier, wherein the carrier comprises the base sequence, or a composite unit connecting the base sequence with a corresponding signal sequence. The invention also relates to an amino acid sequence coded by the basesequence, and further relates to a technical method for preparing single enantiomorph of mandelic acid and analogue thereof from corresponding mandelonitrile by utilizing the recombinant expression nitrilase as catalyst.
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Description

technical field

[0001] The present invention relates to a base sequence encoding nitrilase, a plasmid containing the base sequence, and a transformant transformed from the plasmid. The present invention also relates to a genetically engineered recombinant nitrilase that can catalyze the efficient and enantioselective hydrolysis of mandelonitrile into (R)-(-)-mandelic acid, and use this enzyme to prepare (R) from the corresponding mandelonitrile -Technical methods of (-)-mandelic acid and its analogues. The present invention also relates to a method for preparing (R)-(-)-mandelic acid and its analogs using the transformant. The present invention also relates to the preparation of corresponding (R)-(-)-mandelic acid single enantiomers and their analogs by utilizing nitrilase transformants, cultures thereof or processed products thereof to catalyze the asymmetric hydrolysis of mandelonitrile Methods. technical background

[0002] Mandelic acid, or α-hydroxyphenylacetic acid,...

Claims

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