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Method for producing phloretin by enzymatic hydrolysis method

A technology of phloretin and enzymatic hydrolysis is applied in the field of enzymatic hydrolysis to produce phloretin, which can solve problems such as residues and organic residues, and achieve the effects of less impurities, easy control and simple production process.

Active Publication Date: 2010-05-05
天津市尖峰天然产物研究开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional method has the deficiency of organic matter residue or acid and alkali residue

Method used

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  • Method for producing phloretin by enzymatic hydrolysis method
  • Method for producing phloretin by enzymatic hydrolysis method
  • Method for producing phloretin by enzymatic hydrolysis method

Examples

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Embodiment 1

[0025] A method for producing phloretin by enzymatic hydrolysis, comprising the steps of:

[0026] (1) dissolving the phlorizin raw material with a mass content of phlorizin of 10% in deionized water at a mass ratio of 1:30;

[0027] (2) Adding sino-β hydrolase activated at pH = 4.0 at a temperature of 40°C for 15min, the mass ratio of the phlorizin raw material to the sino-β hydrolase is 10:1, at 40°C, stirring Next, enzymatic hydrolysis reaction for 72 hours;

[0028] (3) Rapidly heat up to 80°C for 20 minutes to inactivate;

[0029] (4) Separation of solid and liquid, and drying of the solid to obtain phloretin. The enzymolysis rate of phlorizin can reach 70%.

Embodiment 2

[0031] A method for producing phloretin by enzymatic hydrolysis, comprising the steps of:

[0032] (1) dissolving the phlorizin raw material with a mass content of 50% phlorizin in deionized water at 80° C. at a mass ratio of 1:10;

[0033] (2) Adding sino-β hydrolase activated at pH=5.0 and temperature 40°C for 15min, the mass ratio of the phlorizin raw material to the sino-β hydrolase is 20:1, at 60°C, stirring Next, enzymatic hydrolysis reaction for 48 hours;

[0034] (3) Rapidly heat up to 90°C for 15 minutes to inactivate;

[0035] (4) Separation of solid and liquid, and drying of the solid to obtain phloretin. The enzymolysis rate of phlorizin can reach 80%.

Embodiment 3

[0037] A method for producing phloretin by enzymatic hydrolysis, comprising the steps of:

[0038] (1) According to the ratio of 1:50 by mass ratio, the phlorizin raw material with a mass content of phloridzin of 80% is dissolved in 88°C deionized water;

[0039] (2) Adding sino-β hydrolase activated at pH = 4.0 at a temperature of 45°C for 15 minutes, the mass ratio of the phlorizin raw material to the sino-β hydrolase is 4:1, at 40°C, stirring Next, enzymatic hydrolysis reaction for 10 hours;

[0040] (3) Rapidly heat up to 90°C to inactivate for 20 minutes;

[0041] (4) Separation of solid and liquid, and drying of the solid to obtain phloretin. The enzymolysis rate of phlorizin can reach 100%.

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Abstract

The invention discloses a method for producing a phloretin by an enzymatic hydrolysis method, which comprises the following steps of: dissolving phlorizin raw materials with the phlorizin quality content of 10 percent to 80 percent in deionized water; adding activated sino-beta hydrolytic enzyme, stirring and carrying out enzymatic reaction for 5-72 hours at 20-90 DEG C, wherein the weight ratio of the phlorizin raw materials to the sino-beta hydrolytic enzyme is 1-50:1; rapidly raising temperature to 60-100 DEG C for inactivating for 15-25 min; solid-liquid separating, drying the solid to obtain the phloretin. The phlorizin in plants is taken as raw material, and the method has the advantages of easy raw materials acquisition, low price, simple production process, and easy control. In the invention, an aglycon configuration obtained by enzymolysis is not damaged and has stronger biological activity; the enzymatic hydrolysis has strong specifity, and the products obtained have little impurities; because the phloretin obtained by the method is white, the application field of the phloretin is broadened.

Description

technical field [0001] The invention relates to the preparation of phloretin, in particular to a method for producing phloretin by enzymatic hydrolysis. Background technique [0002] Phloretin is a new type of natural skin whitening agent newly researched and developed abroad. It is mainly distributed in the peels and root barks of juicy fruits such as apples and pears. Its moisturizing effect is very strong, and it can absorb 4 to 5 times its own weight in water. It can promote the absorption and utilization of other functional factors in the formula, so that it can exert better efficacy. It has strong anti-oxidation function and can remove free radicals in the skin. The anti-oxidation concentration of oil is (10~30)×10 -6 between. It can prevent sugar components from entering epidermal cells, thereby inhibiting the excessive secretion of skin glands, and treat acne with exuberant secretion; it can inhibit the activity of melanocytes, and has a lightening effect on vario...

Claims

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Application Information

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IPC IPC(8): C12P7/26
Inventor 於洪建刘丹孙华庚陈国华
Owner 天津市尖峰天然产物研究开发有限公司
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