Sugarcane tissue-culture-seedling bare-seedling field-transplanting method
A technology for tissue culture seedlings and sugarcane, which is applied in the field of reproduction and production of sugarcane seedlings, which can solve the problems of occupying a large seedling area, long cultivation time, and high production costs, and achieve the effects of easy seedling raising, convenient transportation, and saving transportation costs
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[0016] The present invention will be further described in detail through the following specific examples, but the field transplanting time and content of the naked seedlings of the present invention are not limited thereto.
[0017] 1. Cultivate strong rooted seedlings
[0018] Using Xintaitang No. 22 as a donor, the shoot tip was detoxified from July to August, and the detoxified tissue culture seedlings were bred. The proliferation medium was MS+6-BA1.0-1.5mg / L+NAA0.1mg / L, Proliferate under the conditions of natural scattered light and temperature of 28°C. When the tissue culture seedlings proliferate to the 5th to 6th generation, they are transferred to hormone-free MS medium and cultured for about 30 days (1 to 2nd generation time), to reduce the proliferation speed, so that the nutrition of the medium can meet the existing conditions to the greatest extent. The tissue culture seedlings grew robustly. The tissue-cultured seedlings cultivated by strong seedlings took root...
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