Total sesquiterpene lactone extract of centipeda minima, preparation method and application thereof

A technology of sesquiterpene lactones and goose-buying herbivores, applied in the field of goose-buying total sesquiterpene lactones and its preparation, can solve the problem of goose-buying without new active sesquiterpene lactones The patents and literatures of the anti-tumor effective parts of grasses have not been found, etc., to achieve the effect of inhibiting tumor cell proliferation and high yield

Inactive Publication Date: 2010-06-16
JINAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Although many studies have shown that sesquiterpene lactones are the anti-tumor active ingredients of geese that do not eat herbivores, no extraction or separation of goose The process research on the total sesquiterpene lactone compounds in the herbivore as its anti-tumor effective part, and there are no patents and literatures on the anti-tumor effective part of the goose not herb containing new active sesquiterpene lactones

Method used

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  • Total sesquiterpene lactone extract of centipeda minima, preparation method and application thereof
  • Total sesquiterpene lactone extract of centipeda minima, preparation method and application thereof
  • Total sesquiterpene lactone extract of centipeda minima, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Preparation of volatile oil of Goose Bubivora: 300g dried whole grass of Goose Bubivora (collected from Guangdong, identified by Mai Zhenqiu, senior engineer of Guangzhou Medicinal Materials Company) was crushed, passed through a 60-mesh sieve, and placed in supercritical CO 2 In the extractor, the extraction pressure was 20 MPa, the extraction temperature was 40° C., the flow rate was 20 liters / hour, and the extraction time was 2 hours, and 15.2 g of volatile oil of Goose Bubivora was obtained.

Embodiment 2

[0035] Preparation of volatile oil of goose herbivore: crush 300g of dried whole grass of goose not herbivore, pass through a 60 mesh sieve, and place in supercritical CO 2 In the extractor, the extraction pressure is 25 MPa, the extraction temperature is 38°C, the flow rate is 20 liters / hour, the concentration of 100mL mass percentage is 95% ethanol as the carrier agent, and the extraction time is 2 hours to obtain 16.7g of goose non-grass volatile oil .

Embodiment 3

[0037] Preparation of the total sesquiterpene lactone extract of goose herbivore: Weigh 150g of D101 macroporous adsorption resin, add 800mL of distilled water, stir evenly, remove the resin monomer or fragments by water flotation, and then place it at room temperature for 24h. After fully swelling, drain the resin; then use ethanol to wet the resin into the column (inner diameter × length = 6 × 100cm, after the column is loaded, the retention volume of the column is 550mL), and flow wash the column with ethanol until the effluent Mix with water (the volume ratio of effluent to water is 1:5) without white turbidity, then fully rinse the column with distilled water until the effluent has no smell of ethanol, and place the column overnight to equilibrate to obtain a macroporous resin column.

[0038] Take by weighing 3.8g of the volatile oil of goose not herbivorous obtained in Example 1, dissolve it in 30mL of 95% ethanol by mass percentage, after ultrasonic degassing, add in th...

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Abstract

The invention provides a total sesquiterpene lactone extract of centipeda minima, a preparation method and application thereof. The preparation method comprises the following steps: crushing dried total centipeda minima, and extracting centipeda minima volatile oil by a supercritical carbon dioxide extracting method; and enriching the centipeda minima volatile oil through macroporous adsorption resin column chromatography or silica gel column chromatography to obtain the total sesquiterpene lactone extract of the centipeda minima. According to the method, the total sesquiterpene lactone with effect of inhibiting tumor proliferation is extracted from Chinese medicament centipeda minima and is enriched for the first time; the extract is rich in short-leaf geraniin; the extract contains at least nine sesquiterpene lactone compounds, wherein the angelic acid centipeda minima and the isobutyric acid centipeda minima are new active sesquiterpene lactone compounds; by the preparation method, the yield is high; the method is suitable for industrialized production; and the extract has the effect of inhibiting tumor cell proliferation, and can be used for preparing various medicaments or health-care products with anti-tumor effect.

Description

technical field [0001] The invention belongs to the field of preparation of active ingredients of traditional Chinese medicines, and in particular relates to an extract of total sesquiterpene lactones of goosebubivorous and its preparation method and application. Background technique [0002] Goose does not eat grass is the whole plant with flowers of Centipeda minima (L.) A.Br.et.Aschers, a plant of Compositae. Goose does not eat grass and is used as medicine. There are also records. In my country, geese do not eat grass and are mainly produced in Zhejiang, Hubei, Jiangsu, Guangdong and other places, and are also distributed in Guangxi, Henan, Jiangxi, Fujian, Anhui and other places. The herbal medicine was recorded in the 2000 and 2005 editions of "Chinese Pharmacopoeia" respectively. [0003] The chemical components of goose herbivore mainly include volatile oils, terpenes, flavonoids and sterols, among which flavonoids and sesquiterpenoids are considered to be the acti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28C07D493/10A61K31/365A61P35/00
CPCY02P20/54
Inventor 李药兰叶文才张晓琦王英吴鹏
Owner JINAN UNIVERSITY
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