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Method for culturing inonotus obliquus by fermentation

A technology of Inonotus obliquus and a culture solution, which is applied in the field of fermentation and cultivation of Inonotus obliquus, can solve the problems such as the decline of the pharmacological activity of Inonotus obliquus, and achieves wide practical value and economic value, low cost, accumulation and anti-oxidation. activity-enhancing effect

Inactive Publication Date: 2010-06-23
XUZHOU NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the absence of bacterial and mold invasion and insect phagocytosis pressure in the artificial culture environment, the accumulation of phenolic compounds in Inonotus obliquus is minimal, and the pharmacological activity of the artificially cultured Inonotus obliquus is greatly reduced (Zheng W, Zhao Y, Zhang M , Yin Z, Chen C, Wei Z. Phenolic compounds from Inonotus obliquus and their immune-stimulating effects. Mycosystema 2008, 27: 39-47.)

Method used

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  • Method for culturing inonotus obliquus by fermentation
  • Method for culturing inonotus obliquus by fermentation
  • Method for culturing inonotus obliquus by fermentation

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Embodiment 1

[0013] Embodiment 1, the fermentation culture of Inonotus obliquus

[0014] 1) Preparation of Alternaria cell wall fragments

[0015] Alternaria cell wall fragments (ACW) were prepared as follows:

[0016] Alternaria was purchased from the China General Microorganism Culture Collection and Management Center (CGMCC 3.5373), Beijing Institute of Microbiology, Chinese Academy of Sciences, and inserted into a 500-milliliter triangular flask containing 200 milliliters of Alternaria culture medium, at 26 ° C, 140 rpm / min in a constant temperature shaker for 3-4 days. Alternaria medium: glucose 2g / 100ml, peptone 0.5g / 100ml, MgSO 4 0.01g / 100ml, KH 2 PO 4 0.001g / 100ml, pH 6.0. After 3-4 days, filter the Alternaria culture solution, wash the obtained mycelia three times with ultrapure water, and then use 250w ultrasonic wave in ultrapure water for 30 minutes. Centrifuge the ultrasonically disrupted Neurospora mycelia under the condition of 4800×g gravity for 20 minutes, discard...

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Abstract

The invention discloses a method for culturing inonotus obliquus by fermentation, which comprises the step of adding bacteria-free cell wall fragments of Alternaria bacteria into culture solution of the inonotus obliquus, wherein the cell wall fragments of Alternaria bacteria are obtained by crushing and centrifuging the mycelium of the Alternaria bacteria. The inonotus obliquus is cultured by adding the cell wall fragments of Alternaria bacteria to fermentation liquor for fermentation culture of the inonotus obliquus; and the accumulation and the antioxidant activity of intracellular and extracellular phenol compounds of the obtained inonotus obliquus are obviously improved. The method for culturing the inonotus obliquus by fermentation has the advantages of low consumption of cell wall fragments of Alternaria bacteria, simple preparation method, low cost, and high practical value and economic value.

Description

technical field [0001] The invention relates to a method for fermenting and cultivating Inonotus obliquus. Background technique [0002] Inonotus obliquus is a medicinal fungus belonging to Basidiomycotina, Phytomycetes, Nonbryomycetes, Polyporaceae, and Phytophthora genus. It parasitizes on the trunk of birch trees and has small fruiting bodies. , black lumps, infertile. Collected in the wild are usually black nodules formed by mycelium, the medicinal part of the fungus. Inonotus obliquus is mainly distributed in northern Russia, Northern Europe, Japan (Hokkaido) and my country's Changbai Mountains and Daxing'an Mountains and other regions at 40°-50° north latitude (Yellow Years. Russia's mysterious folk medicinal fungus-Inonotus obliquus. Chinese food Bacteria, 2002, 21: 7.). Inonotus obliquus is widely used to treat malignant tumors in Russia, Northern Europe, Japan, the United States and Korea (Glowacki, Tomaszewski J. Attempted therapy of inoperable cancer of the fema...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/22C12R1/645
Inventor 郑维发赵艳霞缪康杰张梅梅魏志文
Owner XUZHOU NORMAL UNIVERSITY