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Preparation of clathrate compound containing isoflavone and cyclodextrin of chickpea

A technology of cyclodextrin inclusion compound and isoflavone, which is applied in the field of preparation of natural herbal drug inclusion compound, can solve the problems of poor water solubility, achieve high water solubility, improve stability, and have good application prospects

Inactive Publication Date: 2010-06-30
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its water solubility is poor and cannot be well absorbed by the human body.

Method used

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  • Preparation of clathrate compound containing isoflavone and cyclodextrin of chickpea
  • Preparation of clathrate compound containing isoflavone and cyclodextrin of chickpea
  • Preparation of clathrate compound containing isoflavone and cyclodextrin of chickpea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Preparation of chickpea isoflavone samples

[0028] Grind the chickpeas into powder and dry them. Put 50g of soybean powder into a 500ml three-necked bottle, add 400ml of petroleum ether, and place in a 70°C water bath to reflux for 1.5 hours. After the reflux is completed, the product is suction-filtered, the obtained defatted soybean powder is dried in an oven, and the filtrate is distilled in a rotary evaporator to recover petroleum ether. Put the dried defatted soybean powder into a 500ml three-neck bottle, add 300ml of ethanol with a mass fraction of 65%, place it in a water bath at 85°C and reflux for 1.5 hours, and reflux twice. After the reflux was completed, the product was subjected to suction filtration, and the two reflux filtrates were combined, and placed in a rotary evaporator for distillation to recover ethanol. Add 50ml of distilled water to the crude isoflavones, pour it into a macroporous adsorption column, adjust the flow rate to 1ml / min, and co...

Embodiment 2

[0035] (1) Preparation of chickpea isoflavone samples

[0036] Grind the chickpeas into powder and dry them. Put 50g of soybean powder into a 500ml three-necked bottle, add 400ml of petroleum ether, and place in a 70°C water bath to reflux for 1.5 hours. After the reflux is completed, the product is suction-filtered, the obtained defatted soybean powder is dried in an oven, and the filtrate is distilled in a rotary evaporator to recover petroleum ether. Put the dried defatted soybean powder into a 500ml three-neck bottle, add 300ml of ethanol with a mass fraction of 65%, place it in a water bath at 85°C and reflux for 1.5 hours, and reflux twice. After the reflux was completed, the product was subjected to suction filtration, and the two reflux filtrates were combined, and placed in a rotary evaporator for distillation to recover ethanol. Add 50ml of distilled water to the crude isoflavones, pour it into a macroporous adsorption column, adjust the flow rate to 1ml / min, and co...

Embodiment 3

[0043] (1) Preparation of chickpea isoflavone samples

[0044]Grind the chickpeas into powder and dry them. Put 50g of soybean powder into a 500ml three-necked bottle, add 400ml of petroleum ether, and place in a 70°C water bath to reflux for 1.5 hours. After the reflux is completed, the product is suction-filtered, the obtained defatted soybean powder is dried in an oven, and the filtrate is distilled in a rotary evaporator to recover petroleum ether. Put the dried defatted soybean powder into a 500ml three-neck bottle, add 300ml of ethanol with a mass fraction of 65%, place it in a water bath at 85°C and reflux for 1.5 hours, and reflux twice. After the reflux was completed, the product was subjected to suction filtration, and the two reflux filtrates were combined, and placed in a rotary evaporator for distillation to recover ethanol. Add 50ml of distilled water to the crude isoflavones, pour it into a macroporous adsorption column, adjust the flow rate to 1ml / min, and con...

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Abstract

The invention relates to preparation of clathrate compound containing isoflavone and cyclodextrin of chickpea, comprising the following steps: grinding the chickpea into powder, drying, adding petroleum ether, backflowing for 1.5 hours in water bath with the temperature of 70 DEG C, filtering and drying; adding 65% alcohol, putting the obtained mixture in water bath with the temperature of 85 DEG C, backflowing for 1.5 hours, carrying out backflowing for two times, and filtering; adding distilled water, pouring the distilled water into a macroporous absorbing column, adjusting the flow rate to be 1ml / min, using a beaker to receive samples passing the column; adding 70% alcohol, adjusting the flow rate to be 0.5ml / min, collecting flow liquid, and distilling to obtain pure flavone; charging cyclodextrin and pure isoflavone with the mass ratio of 4-6:1, adding 2-4 times of water under normal temperature, grinding the mixture to be pasty, vacuum-drying, cleaning with alcohol, and drying to obtain the clathrate compound. The preparation has simple method, low cost, little toxicity of selected microcapsule wall materials, can increase the solubility of botanical drug after clathrate, improve the bioavailability of isoflavone and has good application prospect.

Description

technical field [0001] The invention belongs to the field of preparation of inclusion compounds of natural herbal medicines, in particular to the preparation of inclusion compounds of chickpea isoflavones and cyclodextrins. Background technique [0002] Chickpea is one of the edible legumes with the largest planting area in the world, the second most consumed legume in the world, and one of the main foods in barren and arid regions. [0003] Chickpeas are rich in isoflavones such as biochanin A and formononetin, genistein, calycosin, formononetin and Indian dalcoside, which can reduce the main force of the brain and peripheral blood vessels, lower blood pressure, improve Cerebral circulation and coronary circulation, promoting collateral circulation of ischemic myocardium, etc., can also reduce the production of lactic acid in ischemic myocardium, reduce myocardial ischemic damage, and have estrogen-like effects, and can be used to treat breast cancer and uterine cancer. Ho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/48A61K31/352A61P9/10A61P9/12A61P35/00A61K47/69
Inventor 赵曙辉李晶周建军
Owner DONGHUA UNIV
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