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Pretreatment and quantitative detection method for detecting tobacco flavor components

A quantitative detection method and a technology of tobacco flavors, which are applied in the pre-treatment of tobacco flavor components and the field of quantitative detection of flavor components, can solve the problems of poor reproducibility of detection, low detection efficiency, detection interference of flavor substances, etc. , to achieve the effect of quality monitoring, sensitive detection and good reproducibility

Active Publication Date: 2011-08-24
CHINA TOBACCO GUANGDONG IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many pretreatment methods for tobacco flavor samples. Through the detection experiment of tobacco flavor, it is found that simultaneous distillation extraction (SDE), steam distillation (WSD) and supercritical fluid extraction (SCF) are relatively complicated. The detection efficiency is low; direct sampling analysis of flavors will produce obvious peak information, which will interfere with the detection of fragrance substances; the use of solvent dilution method will reduce the detection sensitivity of a large number of small peak substances; headspace analysis (HS) will greatly increase the amount of sample information Reduce; the internal standard substance selection difficulty of headspace solid-phase microextraction method (SPME), the reproducibility of detection is poor; And liquid-liquid extraction method is relatively simple, can eliminate the influence of bag peak material, reproducibility is good, so the present invention selects for use Analysis of Tobacco Essence Samples by Liquid-Liquid Extraction
[0004] Now when the liquid-liquid extraction method is used for the pretreatment of the tobacco essence, dichloromethane etc. are generally used as the extractant. Less, not sensitive enough

Method used

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  • Pretreatment and quantitative detection method for detecting tobacco flavor components
  • Pretreatment and quantitative detection method for detecting tobacco flavor components
  • Pretreatment and quantitative detection method for detecting tobacco flavor components

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Take 1 mL of tobacco flavor, which is a product of Huafang Tobacco Flavor Co., Ltd., wherein the mass content of propylene glycol, glycerin and sugar is ≤1%.

[0035] Add 1 mL of ethyl acetate to the flavoring for tobacco, oscillate and shake well, then let it stand still, the mixed liquid is layered, and the organic layer is light yellow, and the liquid in the organic layer is sampled for quantitative detection by GC-FID.

[0036] Agilent (Agilent) 6890GC-FID was used, the chromatographic column was HP-5MS, 30m×0.25μm×0.25mm; the temperature of the injection port was 280°C; the carrier gas was helium, and the flow rate was 1mL / min; the injection was split injection, The split ratio is 20:1; the temperature is programmed, the initial furnace temperature is 40°C, and the temperature starts to rise after keeping for 5 minutes. After the temperature is raised to 150°C at a heating rate of 5°C / min, the temperature is raised to 280°C at a heating rate of 8°C / min. Hold for 15...

Embodiment 2

[0039] Take 1 mL of the same tobacco flavor as in Example 1, add 1.5 mL of ethyl acetate to the tobacco flavor, oscillate and shake well and let it stand, the mixed liquid is layered, wherein the organic layer appears light yellow, and the organic layer liquid is taken into Samples were quantified using GC-FID.

[0040] The detection conditions are the same as in Example 1, please refer to the obtained GC-FID figure figure 2 , figure 2 It is the GC-FID diagram of the tobacco flavor with the volume ratio of tobacco flavor and ethyl acetate in the pretreatment as 1:1.5.

Embodiment 3

[0042]Take 1mL of the same tobacco flavor as in Example 1, add 2mL of ethyl acetate to the tobacco flavor, oscillate and shake well, then let it stand, and the mixed liquid is layered, wherein the organic layer is light yellow, and the organic layer liquid is taken as a sample , using GC-FID for quantitative detection.

[0043] The detection conditions are the same as in Example 1, please refer to the obtained GC-FID figure image 3 , image 3 It is the GC-FID diagram of the tobacco flavor with the volume ratio of tobacco flavor and ethyl acetate in the pretreatment as 1:2.

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Abstract

The invention provides a pretreatment method for detecting tobacco flavor components, which comprises the following steps: adding ethyl acetate to a tobacco flavor to be detected, and then shaking up; taking out an organic-layer liquid, with the volume ratio of the tobacco flavor and the ethyl acetate of 1:1 to 1:2, and the mass content of propylene glycol, glycerin and sugar in the tobacco flavor of less than or equal to 1 percent. The pretreatment method and quantitative detection method provided by the invention can eliminate the impact of peak envelope substance in the tobacco flavor and distinguish among more flavor components, and has more peaks of fragrant substance, sensitive detection and good reproducibility, thereby effectively monitoring the quality of the flavor.

Description

technical field [0001] The invention relates to a detection technology of flavor components for tobacco, in particular to a pretreatment method for detection of flavor components for tobacco and a quantitative detection method for flavor components. Background technique [0002] Tobacco flavors are mostly in liquid state, with obvious peak information, propylene glycol, glycerin and sugar content ≤ 1%, and other flavor substances with relatively large peak information. The most important thing in the detection of various indicators of tobacco flavors is aroma quality assessment. If the evaluation of aroma quality is done by human sensory evaluation, there are relatively large differences in the results due to the subjectivity of human sensory evaluation and uncertain factors. [0003] The use of efficient and sensitive modern analytical separation technologies such as mass spectrometry and chromatography enables people to separate more complex mixtures and obtain a large am...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N30/02G01N30/30G01N30/68
Inventor 孔浩辉陈翠玲周瑢
Owner CHINA TOBACCO GUANGDONG IND