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Device for exciting fluorescent sample by utilizing visible light

A technology for exciting fluorescence and visible light, which is applied in the fields of fluorescence/phosphorescence, material excitation analysis, etc., and can solve the problems of limited application range of blue light transillumination box

Active Publication Date: 2010-07-21
陈翰民 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, when using a blue light transillumination box, weak fluorescent signals in protein gels are sometimes difficult to detect by direct observation, thus limiting the scope of application of the blue light transillumination box

Method used

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  • Device for exciting fluorescent sample by utilizing visible light
  • Device for exciting fluorescent sample by utilizing visible light
  • Device for exciting fluorescent sample by utilizing visible light

Examples

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Embodiment Construction

[0023] Materials and Methods - Preliminary Procedures

[0024] A commercial standard protein mix was obtained (GE Healthcare, Piscataway, NJ, USA) containing rabbit muscle glycogen phosphorylase b (GP), bovine serum albumin (BSA), egg ovalbumin (OVA) , bovine erythrocyte carbonic anhydrase (CA), soybean trypsin inhibitor (TI) and bovine lactalbumin (LAC). The protein mixture was serially diluted two-fold to reduce the total protein content from 4,000 ng to 7.8 ng, and then the protein mixture was separated by 15% polyacrylamide gel electrophoresis (SDS-PAGE). All electrophoresis procedures were performed according to standard protocols with minor modifications. Then use any of SYPRORuby, SYPRO Tangerine, SYPRO Orange (Invitrogen, Grand Island, New York, USA) and Deep Purple (GE Healthcare, Piscataway, New Jersey, USA) according to the manufacturer's instructions Electrophoresis protein gel.

[0025] Gel imaging and gel image analysis

[0026] The backlit blue light panel i...

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PUM

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Abstract

The invention relates to a device for exciting a fluorescent sample by utilizing visible light, in particular to a device for lightening a fluorescent signal of polyacrylamide protein gel or other similar objects by a backlight-type blue light plate so as to improve a signal-to-noise ratio in the exciting process. The embodiment of the invention shows that the device is suitable for observing a plurality of protein gels dyed by fluorescer, and can ensure that certain weak fluorescent signals can be inspected by naked eyes and a filter lens is not needed. The device for straightly observing the fluorescent signal of the protein gel by utilizing the backlight-type blue light plate comprises a transparent plate for guiding light and at least one light source, wherein the at least one light source is arranged beside the transparent plate so that the fluorescent sample placed on the transparent plate of the device can be excited by light refracted or reflected by the transparent plate according to the Snell's law; and the signal-to-noise ratio in the exciting process is improved through the light refraction effect of the device.

Description

technical field [0001] The present invention relates to devices for exciting fluorescent samples using visible light. Specifically, the present invention relates to a device for exciting a biological sample stained with a fluorescent agent with visible light using Snell's Law to improve the signal-to-noise ratio during the excitation process. Background technique [0002] Proteomics may be one of the most attractive disciplines in the current biological science research field. With great progress in high-throughput separation and identification technologies, proteomics can be used to identify proteins that have differential expression or have been modified in different ways between the experimental group and the control group or between pathological samples and healthy samples. Protein is the terminal expression molecule in the signal transmission process, which can more directly respond to the physiological response of cells than its nucleotide precursors, so it has attrac...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 陈翰民陈立明
Owner 陈翰民
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