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Method for rapid determination of tannin content in Slvia Miltiorrhiza Bunge extracting solution

A technology for rapid determination and extraction of liquids, which can be used in the measurement of color/spectral properties, medical preparations containing active ingredients, pharmaceutical formulations, etc. problems, to achieve the effect of simple operation, convenient and easy-to-obtain instruments and equipment, and fast measurement speed

Inactive Publication Date: 2010-08-04
ZHEJIANG UNIV
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The traditional tannin content determination method using casein as the adsorbent and phosphomolybdotungstic acid as the color reagent is cumbersome and time-consuming
Far from meeting the requirements for analysis and monitoring of tannin content in the production process of Danshen injection

Method used

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  • Method for rapid determination of tannin content in Slvia Miltiorrhiza Bunge extracting solution
  • Method for rapid determination of tannin content in Slvia Miltiorrhiza Bunge extracting solution
  • Method for rapid determination of tannin content in Slvia Miltiorrhiza Bunge extracting solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Method flow see figure 1 .

[0052] 1. Calibration set sample collection:

[0053] A total of 53 samples of Danshen extract were collected from 22 batches of 22 batches of the first extraction, 17 extractions of the second extraction, and 14 extractions of the third extraction, as the calibration set samples.

[0054] 2. Determination of the reference value of the calibration set sample:

[0055] The phosphomolybdotungstic acid / casein method in Appendix XB of the Chinese Pharmacopoeia of the 2005 edition was used to determine the tannin content of the calibration set samples, and the color reaction time was uniformly controlled to 30 minutes. The distribution range of tannin content in calibration set samples was 11.2-303.5 μg / ml.

[0056] 3. Calibration set sample UV spectrum data collection:

[0057] Take the calibration set sample, quantitatively dilute 100 times with water, mix, filter, take the filtrate, put it in a 0.5cm quartz cuvette, and use a UV-visible sp...

Embodiment 2

[0076] According to the method of Example 1, the difference is that the partial least squares regression (PLS) method is used to establish a tannin quantitative calibration model, and the leave-one-out method is used for full crossover for internal validation. Quantitative analysis using Unscrambler9.6 software.

[0077] 1. Select the same calibration set sample data as in Example 1.

[0078] 2. Use the Unscrambler9.6 software to select the band of the spectrum. It can be seen from Table 3 that the selection of the band has no significant impact on the performance of the model established by the PLS method. PLS regression selects the 250nm ~ 480nm band to establish the tannin content model tannin, and the model performance is relatively optimal, that is, the correlation coefficient R 2 The maximum is 0.937, and the minimum RMSECV is 18.6μg / ml. The 250-480nm band was selected to establish the quantitative regression model of tannin by PLS method. The correlation diagram betw...

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Abstract

The invention provides a method for the rapid determination of tannin content in a Slvia Miltiorrhiza Bunge extracting solution, comprising the following steps: firstly, collecting Slvia Miltiorrhiza Bunge extracting solutions with different decocting times and different extract processes as calibration set samples; and then utilizing a phosphomolybdium tungstic acid / casein method to measure the tannin content, and taking the measuring result as a reference value; simultaneously, adopting an ultraviolet spectrophotometer to measure the spectroscopic data of the diluents of the calibration set samples within the range of 200-480nm; applying the stoichiometry technology to build a quantitative calibration model for the ultraviolet spectrum of the Slvia Miltiorrhiza Bunge extracting solution and the tannin content thereof; meauring the ultraviolet spectrum data of the Slvia Miltiorrhiza Bunge extracting solution sample the tannin content of which is unknown according to the same method; and utilizing the established quantitative calibration model to rapidly figure out the content value of the tannin. The method of the invention has the characteristics of simple instruments, simple and convenient operation and rapid measurement speed, saves a great amount of labor power and material resources, can be used in rapid measurement of the tannin content during the process of producing Slvia Miltiorrhiza Bunge injecta.

Description

technical field [0001] The invention relates to a method for monitoring the production quality of traditional Chinese medicines, in particular to an ultraviolet spectrum detection method for quickly measuring the tannin content in the salvia miltiorrhiza extract. Background technique [0002] Tannins are a class of water-soluble polyphenolic compounds widely present in Chinese herbal medicines, which have the function of precipitating proteins. The compound is a plant polyphenol composed of glucose (and other polyol) ester of gallic acid (or its polymer), a polymer of flavanol and its derivatives, and a mixture of the two. Tannins in traditional Chinese medicine injections will not only affect the stability and clarity of the preparation, but may also cause a series of serious physiological reactions. For example, some tannins injected into the body will cause a series of clinical symptoms such as jaundice and liver necrosis. Therefore, tannins are often removed as impuriti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/537A61P9/10G01N21/33A61K125/00
Inventor 瞿海斌程翼宇黄红霞
Owner ZHEJIANG UNIV
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