Method for rapid determination of tannin content in Slvia Miltiorrhiza Bunge extracting solution

A technology for rapid determination and extraction of liquids, which can be used in the measurement of color/spectral properties, medical preparations containing active ingredients, pharmaceutical formulations, etc. problems, to achieve the effect of simple operation, convenient and easy-to-obtain instruments and equipment, and fast measurement speed
CN101791331AInactive Publication Date: 2010-08-04ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2010-08-04
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a method for the rapid determination of tannin content in a Slvia Miltiorrhiza Bunge extracting solution, comprising the following steps: firstly, collecting Slvia Miltiorrhiza Bunge extracting solutions with different decocting times and different extract processes as calibration set samples; and then utilizing a phosphomolybdium tungstic acid / casein method to measure the tannin content, and taking the measuring result as a reference value; simultaneously, adopting an ultraviolet spectrophotometer to measure the spectroscopic data of the diluents of the calibration set samples within the range of 200-480nm; applying the stoichiometry technology to build a quantitative calibration model for the ultraviolet spectrum of the Slvia Miltiorrhiza Bunge extracting solution and the tannin content thereof; meauring the ultraviolet spectrum data of the Slvia Miltiorrhiza Bunge extracting solution sample the tannin content of which is unknown according to the same method; and utilizing the established quantitative calibration model to rapidly figure out the content value of the tannin. The method of the invention has the characteristics of simple instruments, simple and convenient operation and rapid measurement speed, saves a great amount of labor power and material resources, can be used in rapid measurement of the tannin content during the process of producing Slvia Miltiorrhiza Bunge injecta.
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Description

technical field

[0001] The invention relates to a method for monitoring the production quality of traditional Chinese medicines, in particular to an ultraviolet spectrum detection method for quickly measuring the tannin content in the salvia miltiorrhiza extract. Background technique

[0002] Tannins are a class of water-soluble polyphenolic compounds widely present in Chinese herbal medicines, which have the function of precipitating proteins. The compound is a plant polyphenol composed of glucose (and other polyol) ester of gallic acid (or its polymer), a polymer of flavanol and its derivatives, and a mixture of the two. Tannins in traditional Chinese medicine injections will not only affect the stability and clarity of the preparation, but may also cause a series of serious physiological reactions. For example, some tannins injected into the body will cause a series of clinical symptoms such as jaundice and liver necrosis. Therefore, tannins are often removed as impuriti...

Claims

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