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Method for preparing saponin by extracting asparagus officinalis L

A technology for asparagus and saponins, which is applied in the field of extraction and preparation of asparagus saponins, can solve problems such as being unsuitable for industrial production, improperly controlling conditions, easily causing accidents and the like

Active Publication Date: 2012-01-18
北京化药科创医药科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research on the extraction and purification of asparagus saponins in my country is still in its infancy. Chinese patent CN100365011C discloses a production method of asparagus saponins. Diethyl ether and petroleum ether are volatile and flammable substances. Improper control of conditions during industrialization can easily cause accidents and cause irreparable losses, so they are not suitable for industrial production

Method used

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  • Method for preparing saponin by extracting asparagus officinalis L
  • Method for preparing saponin by extracting asparagus officinalis L
  • Method for preparing saponin by extracting asparagus officinalis L

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Get 2 kg of fresh asparagus, mash it, add 95% ethanol, adjust the concentration of extraction solvent ethanol in the asparagus to be 60%, extract at 80°C for 1 hour, filter, and extract the filter residue twice with 60% ethanol, each time After 0.5 hours, filter and combine the filtrates, concentrate the filtrate under reduced pressure to a relative density of 1.10, and extract the concentrated solution with n-butanol saturated with water (the volume ratio of the medicinal solution to n-butanol is 1:3), until the color of the n-butanol layer Lighten, combine the n-butanol extraction part, wash the n-butanol extraction part twice with n-butanol-saturated water, discard the water wash, concentrate the n-butanol part under reduced pressure, evaporate the organic solvent n-butanol liquid, to the concentrated solution If there is no n-butanol in the medium, add the concentrated solution to the AB-8 macroporous adsorption resin column, first elute with distilled water for 4 co...

Embodiment 2

[0017] Get 1000g of dried asparagus, pulverize, add 6 times the amount of 65% ethanol to extract at 80°C for 2 hours, filter, and then extract the filter residue twice with 65% ethanol, each time for 1 hour, filter, combine the filtrate, and the filtrate reduces Concentrate under pressure to a relative density of 1.05, extract the concentrated solution with n-butanol saturated with water (volume ratio of medicinal solution to n-butanol is 1:3), until the color of the n-butanol layer becomes lighter, combine the n-butanol extractions, Wash the n-butanol extraction part twice with water saturated with n-butanol, discard the washing liquid, concentrate the n-butanol part under reduced pressure, evaporate the organic solvent n-butanol liquid, until there is no n-butanol in the concentrated solution, add the concentrated solution to On the NKA-9 macroporous adsorption resin column, first elute with distilled water for 4 column volumes, discard the washing solution; use 0.5% NaHCO in...

Embodiment 3

[0019] Assay

[0020] The preparation of need testing solution: get each 20mg of the asparagus saponin extract that makes under embodiment 1, embodiment 2, CN100365011C method, the asparagus extract that prepares under CN101474350A method, put in 100ml volumetric flask, add methanol to dissolve, set Dissolve to the mark and set aside;

[0021] Preparation of reference substance solution: Accurately weigh an appropriate amount of diosgenin reference substance dried under reduced pressure at 60°C to constant weight, add methanol to prepare a solution containing 0.531mg per ml;

[0022] Selection of measurement wavelength: Accurately draw 0.1ml of the reference solution and 1ml of the test solution into a 10ml test tube, evaporate to dryness in a water bath, add 5ml of perchloric acid, shake well, heat in a 70°C water bath for 15min, take it out, and immediately place it in an ice water bath Cool in medium for 2-3min, shake well, use the reagent as blank, and scan in the wavelen...

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Abstract

The invention relates to a method for preparing saponin by extracting asparagus officinalis L. After the asparagus officinalis L medicinal material is concentrated with an ethanol extract, the asparagus offcinalis L is separated with a macroporous adsorption resin to obtain total saponin of the asparagus officinalis L. The method has the advantages of high safety, suitability for industrial production, convenient operation, and high content of the prepared total saponin of the asparagus officinalis L.

Description

technical field [0001] The invention relates to an extraction and preparation method of asparagus saponin, which belongs to the field of preparation of natural medicinal components. Background technique [0002] Asparagus (Asparagus officinalis linn.), scientific name Shi Diaobo, also known as asparagus, asparagus, asparagus, etc., is a perennial herbaceous plant of the genus Asparagus in the family Liliaceae. Asparagus has been cultivated for more than 2,000 years. It originated in the Mediterranean coast and Asia Minor. It was introduced to my country from Europe in the 20th century. Now it is cultivated in vegetable producing areas all over the country. Asparagus is a health-care product with excellent texture and rich nutrition. Vegetables contain essential amino acids, minerals and other nutritional elements, as well as active ingredients such as saponins and flavonoids. Their nutritional value is higher than that of ordinary vegetables and fruits, and they are regarded ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7048A61K125/00
Inventor 关屹闫冬
Owner 北京化药科创医药科技发展有限公司
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