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Method of producing sporophytes of plant belonging to the order laminariales and base material to be used therein

A production method and sporophyte technology, applied in botany equipment and methods, plant cultivation, application, etc., can solve problems such as low attachment rate of seedling substrates, inability to use gametophytes and sporophytes of Laminaria plants, and achieve high efficiency and stability The effect of quality and efficient production

Inactive Publication Date: 2010-09-08
HOKKAIDO UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the above-mentioned conventional culture method, the gametophytes and sporophytes of plants of the order Laminaria cannot be used as seedlings because the adhesion rate to the seedling substrate is low.

Method used

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  • Method of producing sporophytes of plant belonging to the order laminariales and base material to be used therein
  • Method of producing sporophytes of plant belonging to the order laminariales and base material to be used therein
  • Method of producing sporophytes of plant belonging to the order laminariales and base material to be used therein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] In Example 1, it is shown that gametophytes (seedlings) of wrinkled kelp (Laminaria (Saccharina) religiosa) are attached to three substrates of the present invention (granular bodies of sodium polyacrylate gel, granular bodies of polyacrylamide gel, seaweed After being on the granular body of sodium acid gel), the results obtained by observing the gametocyte attachment status and survival status of each base material.

[0081] 1. Modulation of Gametocytes

[0082] Zoospores were isolated from mature sporophytes (Laminaria laminaria) collected naturally. The isolated zoospores were cultured aseptically and stored at the gametophyte stage (ASP 12NTA(-Fe)) culture solution, 14°C, 14 hours light-10 hours dark cycle). Before starting the experiment, the aseptically preserved female gametophytes and male gametophytes were finely cut using a commercially available razor, and screened through a nylon mesh (pore: 100 μm) to prepare subdivided female gametophytes and male gameto...

Embodiment 2

[0100] In Example 2, it shows that the gametocytes (seedlings) of wrinkled kelp are attached to four kinds of substrates of the present invention (granular body of sodium polyacrylate gel, granular body of polyacrylamide gel, surface grafted polyacrylamide gel Glue granules, sodium alginate gel granules), after measuring the number of attached gametophytes and the number of germinated sporophytes of each substrate.

[0101] 1. Modulation of Gametocytes

[0102] By the same procedure as in Example 1, subdivided female gametophytes and male gametophytes were prepared.

[0103] 2. Modulation of Substrate

[0104] (granular body of sodium polyacrylate gel)

[0105] By the same procedure as in Example 1, granular bodies (blocks) of sodium polyacrylate gel were prepared.

[0106] (granular body of polyacrylamide gel)

[0107] By the same procedure as in Example 1, granular bodies (blocks) of polyacrylamide gel were prepared.

[0108] (granular body of surface grafted polyacryla...

Embodiment 3

[0124] In Example 3, after the gametocytes (seedlings) of wrinkled kelp were attached to two substrates of the present invention (seedling ropes coated with sodium polyacrylate gel, and seedling ropes coated with sodium alginate gel) , the results obtained by observing the number of germinated sporophytes of each substrate.

[0125] 1. Modulation of Gametocytes

[0126] By the same procedure as in Example 1, subdivided female gametophytes and male gametophytes were prepared.

[0127] 2. Modulation of Substrate

[0128] (seedling rope coated with sodium polyacrylate gel)

[0129] 4.7 g of sodium acrylate (monomer), 0.3 g of methylenebisacrylamide (crosslinking agent), and 0.13 g of potassium persulfate (initiator) were dissolved in 50 ml of pure water to prepare a sodium acrylate aqueous solution.

[0130] In the above-mentioned sodium acrylate aqueous solution, a "cremona" string cut to a length of 15 cm and having a diameter of 2 mm was soaked for 3 minutes. Then, after t...

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Abstract

A method of producing sporophytes of a plant belonging to the order Laminariales wherein gametophytes or young sporophytes of the plant belonging to the order Laminariales can be used as seedlings. A seedling line coated with a polymer gel or a granular material having the polymer gel on the surface thereof is employed as a seedling base material. As the polymer gel, it is preferable to employ one which allows the adhesion of gametophytes and young sporophytes of the plant belonging to the order Laminariales and on which the gametophytes and sporophytes of the plant belonging to the order Laminariales can grow.It is also preferable that the polymer gel contains nutritional salt(s) capable of promoting the growth and maturation of the gametophytes and sporophytes of the plant belonging to the order Laminariales.

Description

technical field [0001] The invention relates to a method for producing sporophytes of plants of the order Laminaria and a substrate used therein. Background technique [0002] Plants of the order Laminariales include various edible seaweeds such as Laminaria (Saccharina) japonica and Undaria pinnatifida. These Laminariae reproduce by alternating generations of asexual macroscopic sporophytic generations (diploid: 2n) and sexual microscopic gametophytic generations (haploid: n). That is, when diploid sporophytes such as " kelp " and " wakame " that people are familiar with mature, release the zoospore (spore) of haploid (n), and the zoospore (spore) of releasing is attached to the rock etc. on the seabed to form female gametophyte ( female gametophyte) or male gametophyte (male gametophyte). When the female gametophytes mature, they release eggs, and when the male gametophytes mature, they release sperm. The fertilized egg formed by the union of the sperm and the egg grows...

Claims

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Application Information

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IPC IPC(8): A01G33/00
CPCA01G33/00Y02P60/60
Inventor 四仓典滋附柴裕之胜山吉德森冈三果
Owner HOKKAIDO UNIVERSITY