Intelligent electrophoresis temperature control tank and time temperature-gel gradient electrophoresis system

Abstract

The invention relates to an intelligent electrophoresis temperature control tank and a time temperature-gel gradient electrophoresis system comprising the same. The intelligent electrophoresis temperature control tank comprises a tank body, a seal cap and an intelligent temperature controller, wherein a heating device, an electrode connector and a buffer solution circuiting pipe are arranged in the tank body; the temperature control tank can control a heater to heat the buffer solution from initial temperature to target temperature at a constant speed in a set temperature rise duration when the buffer solution reaches the initial temperature, and after the buffer solution reaches the target temperature, constant temperature is maintained for the buffer solution. The time temperature-gradient gel electrophoresis system comprises the intelligent electrophoresis temperature control tank, an electrophoresis bracket and a program-controlled electrophoresis device. The intelligent electrophoresis temperature control tank and the electrophoresis system can save electrophoresis time greatly, reduce the uncertainty of denaturant diffusion, and improve the resolution ratio of gel and the repeatability of the result when used for performing time temperature-gradient gel electrophoresis.

Description

technical field [0001] The invention relates to the field of nucleic acid electrophoresis, in particular to a time-temperature-gel gradient electrophoresis (TTGG, Temporal temperature gel gradient electrophoresis) temperature control tank and an electrophoresis system comprising the temperature control tank. Background technique [0002] Based on existing literature, DGGE (Denatured gradient gel electrophoresis, denaturing gradient gel electrophoresis), TGGE (Temperature gradient gel electrophoresis, temperature gradient gel electrophoresis), TTGE (Temporal temperature gradient gel electrophoresis, time temperature gradient gel electrophoresis) and other electrophoresis techniques can be used to detect DNA point mutations and gene diversity. Among them, the DGGE electrophoresis system appeared the earliest and was widely used. Denaturing gradient gel electrophoresis (DGGE) is an electrophoretic technique first proposed by Fischer et al. (1979) and used to detect DNA mutatio...

AI Technical Summary

Problems solved by technology

[0017] Conventional DGGE electrophoresis systems cannot be used for TTGG electrophoresis

This is because different samples require different heating rates during TTGG electrophoresis. The function of the temperature control tank of the existing DDGE electrophoresis system is to heat the buffer to a preset temperature and maintain a constant temperature. The heating rate cannot be adjusted as needed.

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