66 results about "ELECTROPHORESIS INSTRUMENTATION" patented technology

The invention discloses a PCR (Polymerase Chain Reaction) detection primer and method of pseudomonas putida. The primer is that the nucleotide sequence includes a sequence 1 and a sequence 2. The detection method comprises the following steps of: 1) mixing the primer as disclosed in claim 1, a DNA template of bacterium solution to be detected, an Mg<2+> containing PCR buffer solution for PCR amplification, dNTP (diethyl-Nitrophenyl Thiophosphate) and Taq DNA polymerase to obtain a PCR reaction system; 2) transferring the PCR reaction system to a PCR instrument, and then amplifying according to the preset amplification condition; and 3) detecting an amplified segment through electrophoresis after the amplification is done so as to determine the structure. According to the detection method, the PCR method is carried out for detecting the gene segment of pseudomonas putida, thus the accuracy of the detection result is improved; the detection method is sensitive and only needs common PCR instrument and electrophoresis apparatus to reach the purpose of detection; and the detection result can be issued within 2 to 3 hours only.

A method for analyzing a protein and a peptide, includes: providing a capillary for isoelectric focusing; providing a capillary device for separation and analysis having the capillary and a solid-phase extraction column being unified as a single tube-like structure; providing an electrophoresis instrument having the capillary device and the mechanism regulating the pressure difference at both ends of the capillary device; introducing a sample containing a target protein or peptide into the solid-phase extraction column to let the target protein or peptide be adsorbed on the column, and filling the capillary device with a carrier ampholyte solution; starting separation by isoelectric focusing after eluting the target protein or peptide by filling the solid-phase extraction column with electrode solution or acid or base solution, or after firstly eluting the target protein or peptide with an eluting solution containing carrier ampholyte and secondly filling the solid-phase extraction column with electrode solution or acid or base solution; and focusing the eluted target protein or peptide in the capillary for isoelectric focusing.

The invention provides a vertical electrophoresis tank and an electrophoresis apparatus. The vertical electrophoresis tank comprises a slide assembly, a gel frame assembly and a groove body assembly,wherein the slide assembly comprises a large slide and a small slide used for forming a gel preparing cavity, and a lateral opening groove communicated with the bottom of the gel preparing cavity is formed in the large slide; the gel frame assembly comprises a gel frame, a concave sealing member, a first negative electrode conduction member and a first positive electrode conduction member; the groove body assembly comprises a groove body, a pressing mechanism, a second negative electrode conduction member and a second positive electrode conduction member. The conduction of an electrode and thepressing of the slide assembly are synchronously completed by the pressing mechanism to eliminate the single operation of locking and inserting positive and negative electrodes sequentially in the slide assembly of traditional electrophoresis equipment to prevent the leakage of electricity and electric shocks; gel preparing and electrophoresis are completed in the vertical electrophoresis tank, additional gel preparing auxiliary devices are not needed, occupied space of electrophoresis testing equipment is saved, the operation is easier, and externally dripping gel liquid in the gel preparingprocess is accumulated in the groove body, so that the cleanliness of a testing tabletop is improved.