66 results about "ELECTROPHORESIS INSTRUMENTATION" patented technology

A system for monitoring the state and performance of an analysis device, such as a capillary electrophoresis instrument. The system includes software for operating the analysis device and system management software for monitoring the device, generating a report on the state of the device and selecting an appropriate response based on this report. The response can include altering the function of one or more parts of the device, or signaling the need for a repair to be performed, for example.

The invention discloses a loop-mediated isothermal amplification detection primer group, a detection method and a kit of vibrio parahaemolyticus. The detection primer group is as follows: an upstream outer primer F3: 5'-GCAAAGAAACGCTTGGCG-3', a downstream outer primer B3: 5'-TGCATAGCAATGTTGTCGCT-3', an upstream inner primer FIP: 5'-TCTCTCGGGTGGTGGATGGGTTTCGTTACACTCCGTTCGC-3'; a downstream inner primer BIP: 5'-ATGGTTTGCTACTCTCGCACCCTCGGCTGACAAATGGCTCTA-3'. The detection method disclosed by the invention has the advantages of high sensitivity, high specificity, good accuracy rate and short detection time, only 12 hours are taken from sample treatment to result report, no PCR instrument or electrophoresis instrument is needed, the operation process is simple, and compared with other PCR techniques, the loop-mediated isothermal amplification detection primer group is relatively high in specificity and is applicable to detection of primary testing organizations and food companies.

The invention discloses a loop-mediated isothermal amplification (LAMP) detection primer pair of Pseudomonas aeruginosa, a detection method and a detection kit. Aiming at the ecfX gene of Pseudomonas aeruginosa, the invention designs and screens a set of specific detection primer pair, a detection kit containing the detection primer pair and an LAMP detection method utilizing the detection kit. The detection method is adopted to detect a sample to be detected, specially for drinking water to confirm whether the sample contains the special gene segment of Pseudomonas aeruginosa and further confirm whether the sample contains Pseudomonas aeruginosa. The detection kit and detection method disclosed by the invention have high sensitivity and specificity and short detection time, and the wholeprocess from sample processing to result reporting is only 24h; and the polymerase chain reaction (PCR) amplifier and electrophoresis meter are not adopted, the operation process is simple and the method and kit are especially suitable for the self-checking of grassroots detection institutions and drinking water processing enterprises.

The invention relates to a method for using a capillary electrophoresis instrument to detect the diffusion coefficients of a substance in a liquid phase. The invention discloses a plurality of key factors which affect the accuracy of the detection when using the capillary electrophoresis instrument to detect the diffusion coefficients of the substance, then further provides a fast detecting method which is used for the diffusion coefficients of the substance with a certain commonality and can detect the diffusion coefficients of the substance to be detected in water or an organic solvent. The method of the invention associates the diffusion coefficients of the substance with the appearance time of the elution peak of the substance as well as the width of a self-peak and can fast calculate and obtain the diffusion coefficient value of the substance.

The invention discloses loop-mediated isothermal amplification detection primer groups of Escherichia coli 0157, a detection method and a reagent kit. A set of specific detection primer groups, the detection reagent kit containing detection primer groups, the method using the detection reagent kit for detection through loop-mediated isothermal amplification and the detection method for determining whether the Escherichia coli 0157 exists in a sample to be tested are designed and screened for rfbE genes of the Escherichia coli 0157. The detection reagent kit and the detection method have the advantages that the sensitivity is high, the specificity is high, the accuracy rate reaches 99% and the detection time is short, only 8-10 hours are spent from sample treatment to result reporting, no polymerase chain reaction instrument or electrophoresis apparatus is required, the operating process is simple, the problems that traditional detection methods are long in consumed time, traditional cultural methods are low in sensitivity, cross reaction exists during serum agglutination and the like are solved, the detection reagent kit and the detection method are significant to timely and effective handling of food-borne sudden public health events, and the detection reagent kit and the detection method are particularly applicable to self-checking of basic level detection mechanisms and food processing enterprises.

An electrophoresis apparatus in which an electrophoretic channel formed in a planar plate made of a transparent member and having satisfactory flat and smooth surfaces is irradiated with an excitation beam through the bottom surface or the top surfaces of the channel in a direction orthogonal thereto, and fluorescence from a sample is detected through a side surface of the channel, or the channel is irradiated with the excitation beam through a side surface of the channel while fluorescence from the sample is detected through the bottom surface or the top surface of the channel. With this arrangement, background light and stray light can be reduced so as to enhance the accuracy of detection of the electrophoresis apparatus.

The invention is an improved multiplex capillary electrophoresis instrument or module with at least four and preferably six user-accessible vertically stacked drawers. An x-z stage moves samples from the user accessible drawers to the capillary array for analysis. An additional mechanical stage moves the array from side-to-side. The x-z stage, coupled with the additional array stage allows the system to sample all wells of a 384 well plate with a 96-capillary array. A computer program allows users to add capillary electrophoresis jobs to a queue corresponding to the analysis of rows or plates of samples without stopping or interrupting runs in progress.

A sample analysis method with improved separation accuracy is provided. The method relates to a method for analyzing a sample by electrophoresis using an electrophoresis apparatus provided with a channel and a sample reservoir formed in the channel. The method includes: placing the sample in the sample reservoir of the electrophoresis apparatus with the channel being filled with an electrophoresis running buffer; and performing electrophoresis by applying a voltage to both ends of the channel. The concentration of at least one of a) and b) is set to be approximately the same between the sample and the electrophoresis running buffer; wherein a) and b) are defined as follows:a) an ion that moves in the same direction as an analyte in the sample by the electrophoresis and has a smaller degree of mobility than the analyte, andb) an ion that moves in the opposite direction to the analyte.

The invention belongs to biomedical diagnosis, and discloses a method for detecting gonorrhea Neisseria by utilizing a loop-mediated isothermal amplification technology and a sequence of a primer used by the method. By combining the loop-mediated isothermal amplification technology with the fluorochrome development, the method can realize quick isothermal nucleic acid amplification of the specific DNA sequence of gonorrhea Neisseria and result detection on the premise of not using a PCR amplifier, an electrophoresis apparatus or an ultraviolet gel imaging system. The method is simple to operate, and can be used for detecting the gonorrhea Neisseria in urethral secretions of a clinic outpatient or serves as a method for self-check of patients.

The invention discloses a method for quantitatively detecting residual sulfonylurea herbicide trace amount in soil, which comprises the steps of adding soil to be detected into 0.1mol / L sodium bicarbonate water solution with pH7.8, oscillating for 1h, centrifuging for 5min at a speed of 12000r / min, obtaining sediments and primary liquid supernatant, taking the primary liquid supernatant to be adjusted to be in pH2.5 by 0.1M hydrochloric acid water solution, preparing a sample to be detected, and detecting the sample to be detected by a capillary electrophoresis apparatus; and obtaining contents of metsulfuron-methyl, chlorsulfuron and chlorimuron-ethyl in the sample to be detected according to standard curves of the metsulfuron-methyl, the chlorsulfuron and the chlorimuron-ethyl. The method is high in detecting sensitivity, and reaches detection limit with 10-3 microgram / kg grade, wherein the value of the limit of detection with 10-3g / kg grade is higher than that of the detection limit of other methods of an efficient liquid chromatogram and the like (not more than microgram / kg).

The invention relates to a DNA extraction kit for soil urine and a method. The method comprises the following steps: 1) DNA extraction: a urine-containing soil sample is subjected to splitting decomposition with a soil lysate and proteinase K, soil and a liquid supernatant are separated centrifugally, a binding solution is added to the liquid supernatant, a silicon-based DNA adsorbing material is added after the materials are mixed, the silicon-based DNA adsorbing material and a liquid are separated, the silicon-based DNA adsorbing material is rinsed, and DNA is eluted; 2) PCR amplification and electrophoresis: DNA purified in the step 1) is subjected to PCR amplification, and DNA is detected in electrophoresis apparatus. With adoption of the kit and the method, DNA adsorption by silicon dioxide sourced from sample soil is eliminated, so that unnecessary DNA loss is reduced, and high-quality and high-yield DNA from soil urine can be obtained.

The invention provides a method for establishing snake poison fingerprint maps, which is characterized by preparing snake poison freeze-dry powder into an aqueous solution with certain concentration, separating and detecting the aqueous solution by a capillary electrophoresis apparatus under certain condition to obtain the fingerprint maps of various components in the snake poison. The invention also provides a standard map obtained by the method, and the standard map can provide a reliable basis for identifying the snake poison and controlling the inherent quality.

A fully automated high-precision capillary electrophoresis instrument, comprising an electrophoresis system, a sample injection flow path, and an automatic sampling flow path; the sampling flow path comprising a shunt waste bottle, which is connected to a four-way connector, a four-way sample injection valve and a buffer syringe pump; the automatic sampling flow path comprises a sampling needle, a sample tray, a cleaning tank, reagent bottles, a buffer tube, a six-channel liquid dispenser, and a syringe pump. The described capillary electrophoresis instrument has a fast sample injection speed, high accuracy, good reproducibility, and can be widely used in automated analysis of different substances by capillary electrophoresis.

The invention discloses a DNA quantitative method based on rolling circle amplification. The method comprises the following steps: performing rolling circle amplification reaction, restriction enzyme digestion reaction and electrophoresis on DNS standard solutions with different concentrations, and quantitatively analyzing the brightness of an objective strap to obtain a brightness value of the objective strap, thereby acquiring a standard curve between the concentration of the DNA standard solution and the brightness value of the objective strap; and then substituting the brightness value of the objective strap corresponding to the to-be-quantified DNA solution into a standard curve formula, thereby finishing the quantification of the to be quantified DNA. Through the adoption of the method disclosed by the invention, the sensitivity is close to the PicoGreen fluorescent dye quantitative method, an expensive device is unnecessary, and only a water bath pot, an electrophoresis apparatus and a plastic blooming instrument are required. The method disclosed by the invention can be used for quantitatively testing chain-typed DNA in a pg level.

The invention relates to an intelligent electrophoresis temperature control tank and a time temperature-gel gradient electrophoresis system comprising the same. The intelligent electrophoresis temperature control tank comprises a tank body, a seal cap and an intelligent temperature controller, wherein a heating device, an electrode connector and a buffer solution circuiting pipe are arranged in the tank body; the temperature control tank can control a heater to heat the buffer solution from initial temperature to target temperature at a constant speed in a set temperature rise duration when the buffer solution reaches the initial temperature, and after the buffer solution reaches the target temperature, constant temperature is maintained for the buffer solution. The time temperature-gradient gel electrophoresis system comprises the intelligent electrophoresis temperature control tank, an electrophoresis bracket and a program-controlled electrophoresis device. The intelligent electrophoresis temperature control tank and the electrophoresis system can save electrophoresis time greatly, reduce the uncertainty of denaturant diffusion, and improve the resolution ratio of gel and the repeatability of the result when used for performing time temperature-gradient gel electrophoresis.

The invention discloses a loop mediated isothermal amplification detection primer group, a detection method and a kit for enterococcus faecalis. The detection primer group is as follows: an upstream outer primer is F3:5'-GCCATTCCTCATGGAACAG-3'; a downstream outer primer is B 3:5'-CCACGTTATCCATATCACTACA-3'; an upstream inner primer is FIP:5'-CGGTGCCAAAATTAACACCCTTTAGTGAAAAAATCAGGAATCTGTG-3'; a downstream inner primer is BIP:5'-TGCTACCGTATTATTTGGGATTGCAAAGTGCAATTTGTTGGACTA-3'. The detection method has the advantages of high flexibility, strong specificity, high accuracy, short detection time. From sample treatment to report only takes 12 hours, a PCR (polymerase chain reaction) and an electrophoresis apparatus do not need, the operation process is simple, compared with other PCR technology, the detection method has higher specificity, is applicable to self-detection of basic level detection organizations and drinking water processors.

The invention discloses a gel electrophoresis detection method and kit for the lactoferrin content. According to the method, DNA which can be combined with lactoferrin is used as a probe molecule, andthe content of lactoferrin is detected according to different migration rates of a DNA-lactoferrin conjugate and the DNA which is not combined with lactoferrin in agarose gel electrophoresis and thegray value of a band. The method and the kit do not need sample pretreatment, are suitable for direct detection of lactoferrin in milk powder, raw milk and the like, and have strong specificity and high sensitivity. In addition, according to the method, rapid, low-cost and high-throughput detection can be carried out on lactoferrin by using an electrophoresis apparatus without using large instruments such as chromatography / mass spectrometry / spectrum instruments.

The invention discloses a method for quickly distinguishing octodonta nipae from brontispa longissima through a PCR (Polymerase Chain Reaction) detection technology. The method comprises the following steps: enabling specific primers of COI (Cytochrome Oxidase Subunit I) and ITS (Internal Transcribed Spacer) gene segments of the octodonta nipae to be subjected to PCR reaction with DNA (Deoxyribose Nucleic Acid) templates of the octodonta nipae and the brontispa longissima, placing reaction products on an electrophoresis apparatus, and performing agarose gel electrophoresis, thus distinguishing the octodonta nipae from the brontispa longissima through electrophoretic bands. The method is simple, quick and high in accuracy, and plays a significant role in intensive study of the octodonta nipae and the brontispa longissima.

The invention discloses a PCR (Polymerase Chain Reaction) detection primer and method of pseudomonas putida. The primer is that the nucleotide sequence includes a sequence 1 and a sequence 2. The detection method comprises the following steps of: 1) mixing the primer as disclosed in claim 1, a DNA template of bacterium solution to be detected, an Mg<2+> containing PCR buffer solution for PCR amplification, dNTP (diethyl-Nitrophenyl Thiophosphate) and Taq DNA polymerase to obtain a PCR reaction system; 2) transferring the PCR reaction system to a PCR instrument, and then amplifying according to the preset amplification condition; and 3) detecting an amplified segment through electrophoresis after the amplification is done so as to determine the structure. According to the detection method, the PCR method is carried out for detecting the gene segment of pseudomonas putida, thus the accuracy of the detection result is improved; the detection method is sensitive and only needs common PCR instrument and electrophoresis apparatus to reach the purpose of detection; and the detection result can be issued within 2 to 3 hours only.

The invention relates to the field of medical diagnosis, in particular to a capillary electrophoresis-based multi-dye-set nucleic acid analysis method and application thereof. The method comprises thesteps of before electrophoresis is carried out, performing spectral correction respectively on a plurality of fluorescent dye sets to be used so as to obtain spectrum correction matrixes of all the dye sets, and setting a binning method of an image acquisition device according to the fluorescent dye sets adopted by all channel products; segmenting nucleic acid to be detected into fragment compositions containing a plurality of nucleic acid fragments, and labeling the fragment compositions with the plurality of fluorescent dye sets simultaneously or separately, and using a multi-channel capillary array electrophoresis apparatus to perform multi-channel electrophoresis detection on the labeled fragment compositions; obtaining the electrophoresis data of all channels by means of the image acquisition device, and carrying out spectrum unfolding on the electrophoresis data collected by all the channels by using the spectral correction matrixes. The capillary electrophoresis-based multi-dye-set nucleic acid analysis method can enable gene fragment analysis experiments to be more flexible, and reduces the time and cost of the gene fragment experiments.

The invention provides an integrated type electrophoresis apparatus. The integrated type electrophoresis apparatus comprises a power supply unit, a base, at least one electrophoresis tank, an electrophoresis tank tray and an upper cover which are integrated to form a whole body. The electrophoresis tank is directly arranged on the electrophoresis tank tray and a power supply can be switched on; the upper cover covers and then an electrophoresis experiment can be carried out, so that the integrated type electrophoresis apparatus is safe and convenient; the plurality of electrophoresis tanks can be arranged on the electrophoresis tank tray and are used for carrying out experiments simultaneously, so that instrument equipment and various connection circuits are reduced, a space is saved and a whole laboratory is more tidy; the integrated type electrophoresis apparatus further comprises a demisting and cooling circulating system; cooling air flow circulation is formed at front and rear parts in an electrophoresis apparatus box body, so that the inner temperature of the electrophoresis apparatus is kept in a reasonable range; the stability of an experiment result is improved and a condition that mist is formed on the upper cover, caused by the fact that heat is generated by electrophoresis, can be prevented, so that experiment personnel can observe conveniently.

The invention provides a capillary electrophoresis apparatus which can improve the operability and measuring speed. According to the invention, a sensor for identifying the type of sample containers is fixed at the position away from a capillary anode electrode. The sensor is made to be closer to the sample containers by moving a moving stage so that the sample containers disposed on the moving stage can be identified by the sensor. A fixing apparatus for fixing at least a pair of sample containers is provided on the moving stage.

The invention provides a capillary electrophoresis apparatus. The apparatus comprises a sealed cabin outer cylinder and a sealed cabin plug, and the sealed cabin plug comprises a plug cover and a plug body. According to the capillary electrophoresis apparatus provided by the invention, when the plug body is completely inserted into the sealed cabin outer cylinder, the sealed cabin outer cylinder and the sealed cabin plug form a sealed structure, the sample seat is pushed to lift by an electric push rod, so that the capillary and an electrode extend into the sample liquid in a sample cup, and meanwhile, compressed air with preset pressure is introduced into the sealed structure through an air pump, so to make the sample liquid in the sample cup flow into a capillary tube, so that a positive-pressure sample introduction mode is realized, the pressure intensity provided by positive pressure can be higher, the sample introduction efficiency can be effectively improved, and the efficiency of cleaning the inner wall of the capillary tube and filling a buffer solution can be improved; in addition, the pressure intensity of the positive pressure is easier to control, so the sample injection amount can be controlled by controlling the pressure intensity of the positive pressure and the pressurization time according to different requirements, and micro sample injection is realized.

The invention discloses an LAMP primer set for quickly detecting pseudomonas fluorescens producing thermostable lipase in raw milk and a method and belongs to the field of food safety inspection, andbelongs to the field of food safety detection. By taking a pseudomonas fluorescens lipase gene as a target gene, a set of specific detection primer set and an LAMP detection system and a reaction condition which are suitable for the primer set are designed and screened. A conventional detection method is long in time and consumes both manpower and physical materials. The adopted method is high insensitivity, strong in specificity and short in time; the cost is low, the operation process is simple, and a PCR instrument or an electrophoresis apparatus is not needed in the reaction process; andany instrument is not needed in the result judgment aspect. The shortcomings of the conventional method are overcome to a certain degree. The LAMP primer set and the method have significance on quickly detecting the quality of the raw milk. The adopted method is suitable for bed-layer on-line monitoring and field detection.

The invention relates to a method for using a capillary electrophoresis instrument to detect the diffusion coefficients of a substance in a liquid phase. The invention discloses a plurality of key factors which affect the accuracy of the detection when using the capillary electrophoresis instrument to detect the diffusion coefficients of the substance, then further provides a fast detecting methodwhich is used for the diffusion coefficients of the substance with a certain commonality and can detect the diffusion coefficients of the substance to be detected in water or an organic solvent. Themethod of the invention associates the diffusion coefficients of the substance with the appearance time of the elution peak of the substance as well as the width of a self-peak and can fast calculateand obtain the diffusion coefficient value of the substance.

The invention discloses an electrophoresis apparatus convenient for dropwise adding a test solution, which comprises an electrolysis box, a strip-shaped plate, a plastic bottle, a corrugated cylinder,a round box, a threading hole, a conductive wire, an electric plug, a strip-shaped hole, a column deep groove, an internal thread thin pipe, a thin screw, a motor, a sandwich plate, a sealing slidingstrip block, a connecting block, a U-shaped strip sliding seat, a conical dripper, a column hole block, a winding frame and a torsion spring. The electrophoresis apparatus is reasonable in design, can quickly perform electrolysis operation by only changing the variety of sample solutions and conveniently dropwise adding various sample solutions under the condition of ensuring sufficient other auxiliary sample solutions, achieves the effect of conveniently dropwise adding the sample solutions in a time-saving manner, is compact in structure, has the function of automatically loading and unloading the sandwich structure for electrolysis test, solves the problem that a traditional electrophoresis apparatus is complex in installation of a sandwich structure used for electrolytic testing, facilitates automatic winding of the detached conductive wire under the action of a torsion spring after operation is completed, and saves the arrangement time.

The invention relates to a nucleic acid metabolic enzyme activity detection method which comprises the following steps: S1, artificially synthesizing a single-stranded nucleic acid fragment and a primer complementary from the 3'end, and forming a template through annealing reaction; s2, reacting a template, a reaction buffer solution, a fluorescence modified nucleotide derivative or a fluorescence modified nucleotide derivative analogue and a nucleic acid metabolic enzyme to be measured, and purifying to obtain a primary purified product; s3, the primary purification product, a Taq reaction buffer solution, dNTP and Taq DNA polymerase are subjected to a reaction and purification, and a secondary purification product is obtained; s4, analyzing a secondary purification product by using a capillary electrophoresis apparatus; and S5, making a standard curve, and substituting the result in the step S4 into the standard curve to obtain a detection result. The method is quick to operate, accurate in activity detection result and high in sensitivity, and realizes high-throughput detection.

The invention discloses a capillary electrophoresis apparatus. The capillary electrophoresis apparatus comprises a shell unit; a containing cavity is formed in the shell unit; a bracket unit is arranged in the containing cavity; a clamping unit is arranged on the bracket unit; a clamping door is arranged at the position, corresponding to the clamping unit, of the shell unit; the clamping unit canbe replaced through the clamping door; a capillary assembly is arranged in the clamping unit; a sample loading unit is arranged at the position, corresponding to the cathode end of the capillary assembly, of the bracket unit; a sample loading door is arranged at the position, corresponding to the sample loading unit, of the shell unit; and the sample loading unit can replace samples through the sample loading door. According to the capillary electrophoresis system, the clamping unit, the sample loading unit, an optical detection unit and other components are integrated together, and therefore,the system is high in automation degree and can meet increasingly high requirements for the miniaturization, integration and convenience of detection equipment.

The invention provides a vertical electrophoresis tank and an electrophoresis apparatus. The vertical electrophoresis tank comprises a slide assembly, a gel frame assembly and a groove body assembly,wherein the slide assembly comprises a large slide and a small slide used for forming a gel preparing cavity, and a lateral opening groove communicated with the bottom of the gel preparing cavity is formed in the large slide; the gel frame assembly comprises a gel frame, a concave sealing member, a first negative electrode conduction member and a first positive electrode conduction member; the groove body assembly comprises a groove body, a pressing mechanism, a second negative electrode conduction member and a second positive electrode conduction member. The conduction of an electrode and thepressing of the slide assembly are synchronously completed by the pressing mechanism to eliminate the single operation of locking and inserting positive and negative electrodes sequentially in the slide assembly of traditional electrophoresis equipment to prevent the leakage of electricity and electric shocks; gel preparing and electrophoresis are completed in the vertical electrophoresis tank, additional gel preparing auxiliary devices are not needed, occupied space of electrophoresis testing equipment is saved, the operation is easier, and externally dripping gel liquid in the gel preparingprocess is accumulated in the groove body, so that the cleanliness of a testing tabletop is improved.