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Gel electrophoresis detection method and kit for lactoferrin content

A technology for detecting kits and lactoferrin, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of high cost and complicated sample processing, and achieve the effect of low detection cost, simple and fast operation

Active Publication Date: 2019-12-10
SHAANXI UNIV OF SCI & TECH
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  • Claims
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Problems solved by technology

[0006] The present invention aims at the disadvantages of high cost and complicated sample processing in existing methods for detecting lactoferrin content, and provides a high-accuracy, convenient and fast gel electrophoresis detection method and kit for lactoferrin content

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  • Gel electrophoresis detection method and kit for lactoferrin content
  • Gel electrophoresis detection method and kit for lactoferrin content
  • Gel electrophoresis detection method and kit for lactoferrin content

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Embodiment Construction

[0033] The present invention will be further described in detail below with reference to the accompanying drawings and embodiments. The following embodiments are used to explain the present invention, rather than limiting the protection scope of the present invention.

[0034] (1) Lactoferrin detection based on non-fluorescent labeled DNA probe and agarose gel electrophoresis

[0035] (1) Feasibility and specificity

[0036] 1.1.1. Prepare solution

[0037] a. Lactoferrin binds DNA: Take a tube of unopened DNA probe and centrifuge at 3000-4000 rpm for 1 min to prevent the dry DNA probe powder from being lost when the lid is opened. Add TE (1×) buffer to the centrifuged tube on a clean bench. Shake for 5 minutes to aid dissolution, centrifuge to collect the solution to the bottom of the tube. At this time, the concentration is 100μM. In order to prevent repeated freezing and thawing and degradation of the DNA probe when the concentration is too low, aliquot into a stock solution and s...

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Abstract

The invention discloses a gel electrophoresis detection method and kit for the lactoferrin content. According to the method, DNA which can be combined with lactoferrin is used as a probe molecule, andthe content of lactoferrin is detected according to different migration rates of a DNA-lactoferrin conjugate and the DNA which is not combined with lactoferrin in agarose gel electrophoresis and thegray value of a band. The method and the kit do not need sample pretreatment, are suitable for direct detection of lactoferrin in milk powder, raw milk and the like, and have strong specificity and high sensitivity. In addition, according to the method, rapid, low-cost and high-throughput detection can be carried out on lactoferrin by using an electrophoresis apparatus without using large instruments such as chromatography / mass spectrometry / spectrum instruments.

Description

Technical field [0001] The invention belongs to the field of food detection, and relates to a method for detecting lactoferrin content, in particular to an agarose gel electrophoresis detection method and kit for lactoferrin content in milk and dairy products. Background technique [0002] Lactoferrin (LF) is a non-heme iron-binding glycoprotein with a molecular weight of 70-80kD. It is widely present in exocrine or neutrophils such as milk, saliva, and tears. In 1961, Blanche et al. isolated from human milk and named it lactoferrin. Many other mammalian secretions also contain lactoferrin. [0003] Lactoferrin can enhance the body's antibacterial and antiviral abilities, regulate the body's immunity, anti-cancer, anti-oxidation, and increase the absorption of iron, reduce the use of effective iron, increase the bioavailability of iron in intestinal cells, and reduce iron intake Many effects such as biological activity. In the GB 14880-2012 "Standard for the Use of Food Nutritio...

Claims

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Application Information

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IPC IPC(8): G01N27/447G01N33/58G01N33/68
CPCG01N27/447G01N27/44726G01N33/582G01N33/68G01N2333/47
Inventor 徐秦峰姚璐刘金虎孙娜娜徐丹
Owner SHAANXI UNIV OF SCI & TECH
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