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Method for establishing snake poison fingerprint maps and fingerprint maps thereof

A technique for fingerprinting and establishing methods, which is applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of unseen popularization and application, limited small molecules, etc., and achieve the effects of easy mastery, high precision, and simple methods

Inactive Publication Date: 2009-09-23
SHANGHAI INST OF PHARMA IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the objects separated by capillary electrophoresis in China are mostly limited to small molecules. Zhang Xuan of Tsinghua University has tried to use microemulsion capillary electrodynamic chromatography to conduct a preliminary discussion on the separation conditions of proteins, but it has not been widely used.

Method used

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  • Method for establishing snake poison fingerprint maps and fingerprint maps thereof
  • Method for establishing snake poison fingerprint maps and fingerprint maps thereof
  • Method for establishing snake poison fingerprint maps and fingerprint maps thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] 1 Preparation of test solution and reference solution

[0043] Take 11 batches of Agkistrodon Agkistrodon venom, mix them uniformly, dissolve them in 10ml of distilled water, mix them by ultrasonic vibration, centrifuge at a high speed (10000rpm, 10min), and take the supernatant to obtain the test solution. Ready to use.

[0044] Accurately weigh 10 mg of the plasmin reference substance, dissolve it in 1 ml of distilled water as the reference substance solution;

[0045] 2 Electrophoresis conditions

[0046] The uncoated capillary is 75μm×35cm, the effective length is 25cm (Hebei Yongnian Optical Fiber Factory); the ultraviolet detection wavelength is 195nm; the operating voltage is 10kV; the capillary temperature is 18°C; the sampling method is 0.5psi, 5s. Before using a new capillary column, wash the capillary column with methanol, 1mol / L HCl, 1mol / L NaOH, and pure water for 10 minutes each. The capillary column was rinsed with 0.1mol / L NaOH and purified water for ...

Embodiment 2

[0056] 1 Preparation of the test solution

[0057] Take 11 batches of Agkistrodon Agkistrodon venom, mix them uniformly, dissolve them in 10ml of distilled water, mix them by ultrasonic vibration, centrifuge at a high speed (10000rpm, 10min), and take the supernatant to obtain the test solution. Ready to use.

[0058] Accurately weigh 10 mg of the plasmin reference substance, dissolve it in 1 ml of distilled water as the reference substance solution;

[0059] 2 Electrophoresis conditions

[0060] The uncoated capillary is 75μm×35cm, the effective length is 25cm (Hebei Yongnian Optical Fiber Factory); the ultraviolet detection wavelength is 195nm; the operating voltage is 10kV; the capillary temperature is 18°C; the sampling method is 0.5psi, 5s. Before using a new capillary column, wash the capillary column with methanol, 1mol / L HCl, 1mol / L NaOH, and pure water for 10 minutes each. The capillary column was rinsed with 0.1mol / L NaOH and purified water for 10 min before and a...

Embodiment 3

[0071] 1 Preparation of the test solution

[0072] Take 11 batches of Agkistrodon Agkistrodon venom, mix them uniformly, dissolve them in 10ml of distilled water, mix them by ultrasonic vibration, centrifuge at a high speed (10000rpm, 10min), and take the supernatant to obtain the test solution. Ready to use.

[0073] Accurately weigh 10 mg of the plasmin reference substance, dissolve it in 1 ml of distilled water as the reference substance solution;

[0074] 2 Electrophoresis conditions

[0075] The uncoated capillary is 75μm×35cm, the effective length is 25cm (Hebei Yongnian Optical Fiber Factory); the ultraviolet detection wavelength is 195nm; the operating voltage is 10kV; the capillary temperature is 18°C; the sampling method is 0.5psi, 5s. Before using a new capillary column, wash the capillary column with methanol, 1mol / L HCl, 1mol / L NaOH, and pure water for 10 minutes each. The capillary column was rinsed with 0.1mol / L NaOH and purified water for 10 min before and a...

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Abstract

The invention provides a method for establishing snake poison fingerprint maps, which is characterized by preparing snake poison freeze-dry powder into an aqueous solution with certain concentration, separating and detecting the aqueous solution by a capillary electrophoresis apparatus under certain condition to obtain the fingerprint maps of various components in the snake poison. The invention also provides a standard map obtained by the method, and the standard map can provide a reliable basis for identifying the snake poison and controlling the inherent quality.

Description

technical field [0001] The invention relates to a method for establishing snake venom fingerprints and a standard spectrum obtained by the method. Background technique [0002] Snake venom is a kind of substance secreted from the venom glands of various poisonous snakes. It is used as a weapon for poisonous snakes to protect themselves and hunt for food. It is one of the most poisonous animal toxins in the world. The main components of snake venom are proteins and polypeptides, accounting for about 90-95% of dry weight, including a variety of enzymes, such as phosphatase, arginine esterase, proteolytic enzyme, phospholipase A2, kallikrein-like, 5-nucleotidase, L-amino acid oxidase and acetylcholinesterase, etc. 3-12 kinds of non-enzymatically active proteins or polypeptides such as: neurotoxins, membrane active peptides, bradykinin enhancing peptides, bleeding factors, etc. Many of these active ingredients have pharmacological effects and medicinal value, such as thrombin-...

Claims

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Application Information

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IPC IPC(8): G01N33/00
Inventor 苏恒赵文杰冯军魏晓东
Owner SHANGHAI INST OF PHARMA IND
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