Tissue culture method for anemone
A technique of tissue culture and lotus, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of small number of introduced anemone species and limited seedlings, so as to increase the speed of reproduction, improve the stability, and improve the growth rate of seedlings. The effect of uniformity
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Embodiment 1
[0028] (1) Obtaining sterile materials
[0029] Take the germinated young shoots, remove the wrapped leaves, wash them with tap water for 1 hour, put them on the ultra-clean workbench, soak them in ethanol with a mass concentration of 70% for 10 seconds, and then soak them in mercury with a volume concentration of 0.5‰ for 10 minutes. Rinse 4 times with sterile water, use sterile filter paper to blot the water on the surface, then cut into 0.5cm-long segments with axillary buds, and inoculate the segments with MS+6-BA1.0mg / L+NAA0.1mg / L bud induction medium;
[0030] (2) Differentiation and proliferation of buds
[0031] Two weeks after the segments were inoculated on the bud induction medium, the axillary buds began to expand, and yellow-green protrusions appeared. After 3 weeks, bud meristems could be seen. After culturing for another month, the adventitious buds could grow to 2cm, and the adventitious buds were cut off. Transferred to the proliferation medium comprising t...
Embodiment 2
[0040] (1) Obtaining sterile materials
[0041] Take the germinated young shoots, remove the wrapped leaves, wash them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in ethanol with a mass concentration of 75% for 30 seconds, and then soak them in mercury with a volume concentration of 1‰ for 15 minutes. Rinse with sterile water for 5 times, use sterile filter paper to blot the water on the surface, then cut into 1cm-long segments with axillary buds, and inoculate the segments with MS+6-BA3.0mg / L+NAA0.3mg / On the shoot induction medium of L;
[0042] (2) Differentiation and proliferation of buds
[0043] After the segments were inoculated on the bud induction medium for 3 weeks, the axillary buds began to expand, and yellow-green protrusions appeared. After 4 weeks, bud meristems could be seen. After one and a half months of culture, the adventitious buds could grow to 3cm, and the adventitious buds were cut off. Transfer to the proliferation me...
Embodiment 3
[0052] (1) Obtaining sterile materials
[0053] Take the germinated young shoots, remove the wrapped leaves, wash them with tap water for 3 hours, put them on the ultra-clean workbench, soak them in ethanol with a mass concentration of 75% for 50 seconds, and then soak them in mercury with a volume concentration of 2‰ for 30 minutes. Rinse with sterile water for 6 times, use sterile filter paper to blot the water on the surface, then cut into 2cm-long segments with axillary buds, and inoculate the segments with MS+6-BA5.0mg / L+NAA0.5mg / On the shoot induction medium of L;
[0054] (2) Differentiation and proliferation of buds
[0055]After the segments were inoculated on the bud induction medium for 4 weeks, the axillary buds began to expand, and yellow-green protrusions appeared. After 5 weeks, bud meristems could be seen. After 2 months of culture, the adventitious buds could grow to 2cm, and the adventitious buds were cut off. Transfer to the proliferation medium that comp...
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