Method for improving reproduction coefficient of Phalaenopsis hybrid
A technology of reproduction coefficient and phalaenopsis, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of unsatisfactory reproduction coefficient, easy to produce deformed seedlings, and difficulty in satisfying production, so as to reduce manpower, financial resources and quantity Reduced, reproducible effects
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Embodiment 2
[0043] Embodiment 2 implementation material kind is " super group fire bird " (Dtps.Sogo Beach)
[0044] 1. Get the protocorm induced by the pedicel as the explant;
[0045] 2. Separate the produced protocorms into small pieces with one bud as the unit, and inoculate them in the culture medium at an inoculation density of 25 small pieces with one bud as the unit / 75cm 2 Culture substrate; the culture substrate is sterilized peat moss (Sphagnum palustre) by high temperature and high pressure (121°C, 1.0-1.2 atmospheric pressure, 15min);
[0046] 3. Cultivate according to the following conditions after inoculation: the culture temperature is 25°C±2°C, the pH value of the medium is 5.6-5.8, the light time is 12-16 hours, and the light intensity is 2000-2500lux; Filter-sterilized MS culture solution, the dosage is 20mL / time / 75cm 2; The injection cycle is 1 time / week; it needs to be sterilized by high temperature and high pressure (121°C, 1.0-1.2 atmospheres, 20min) and then added...
Embodiment 4
[0054] Embodiment 4 implementation material kind is " super group fire bird " (Dtps.Sogo Beach)
[0055] 1. Get the protocorm induced by the pedicel as the explant;
[0056] 2. Separate the produced protocorms into small pieces with one bud as the unit and inoculate them in the culture medium at an inoculation density of 23 small pieces with one bud as the unit / 75cm 2 Culture substrate; the culture substrate is sterilized peat moss (Sphagnum palustre) by high temperature and high pressure (121°C, 1.0-1.2 atmospheric pressure, 15min);
[0057] 3. Cultivate according to the following conditions after inoculation: the culture temperature is 25°C±2°C, the pH value of the medium is 5.6-5.8, the light time is 12-16 hours, and the light intensity is 2000-2500lux; Filter-sterilized MS culture solution, the dosage is 20mL / time / 75cm 2 ; The injection cycle is 1 time / week; it needs to be sterilized by high temperature and high pressure (121°C, 1.0-1.2 atmospheres, 20min) and then added...
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