Multiple PCR detection primer of enterococcus and method thereof

Abstract

The invention discloses a multiple PCR detection primer of enterococcus, comprising a sequence of the enterococcus primer, a sequence of enterococcus faecalis strain primer and a sequence of enterococcus faecium strain. The invention establishes a multiple PCR method for identifying the enterococcus fast and accurately, greatly simplifies the checking program, and can be used in a number of basic laboratories. The method can identify the enterococcus and faecalis or faecium by one-time PCR, can detect 1ng genomic DNA at least, has high sensitivity and stronger specificity, is beneficial to identification and diagnosis of the enterococcus, has better sensitivity, repeatability and stability. The invention provides a new fast detection method, and also provides test basis and technical means for molecular epidemiology survey of enterococcal infection, study of enterococcus molecule pathogenic mechanism and early diagnosis, research and development of a diagnostic reagent kit and immunologic mechanism of drugs or vaccines.

Description

technical field [0001] The invention relates to a multiplex PCR detection primer and a method, in particular to a multiplex PCR detection primer and a method for Enterococcus. Background technique [0002] Enterococcus is a Gram-positive coccus that mainly exists in the gastrointestinal tract of humans or animals, and is also widely distributed in soil, water, and food. It is an important conditional pathogen. In recent years, due to the increasing number of enterococcal drug-resistant strains, the hospital infection rate of enterococcus continues to increase, causing urinary tract infection, bacteremia, wound infection, infective endocarditis and food poisoning, etc., has become a major cause of hospital infection. important pathogens. Enterococci can infect a variety of animals, and pathogenic enterococci have been isolated from pigs, silkworms, lambs, donkeys and other animals, and studies have shown that enterococci can be transmitted horizontally between humans and ani...

AI Technical Summary

Problems solved by technology

[0003] At present, the standard method for identifying enterococci mainly relies on biochemical tests for phenotypic identification, which requires a lot of time for the preparation of biochemical tubes and judgment of results

In addition, some commercial identification kits such as API Rapid ID 32Srep, BBL Crystal, and Vitek-32, etc., can identify Enterococcus to the species level, and the test results can be effectively interpreted after 24 hours, and are not suitable for rapid identification at the grassroots level At present, the popularity rate of PCR instruments is gradually increasing. Compared with single-plex PCR, multiplex PCR has the advantages of high efficiency, systematization, and economic simplicity. It can simultaneously detect multiple types of target genes in the same PCR reaction tube, which is suitable for adult Group pathogen detection, saving time, saving reagents, saving funds and expenses, can provide more and more accurate diagnostic information for clinics, and it is particularly necessary to establish a multiplex PCR method for rapid identification of species

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