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Oligonucleotide for inhibiting blood-mediated inflammatory reaction in pancreatic islet transplantation

A technology of oligonucleotide and islet transplantation, applied in the field of oligonucleotide, can solve problems such as inability to control blood sugar stably and prevent complications of diabetes

Inactive Publication Date: 2010-11-17
王维 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Diabetes is a serious metabolic disease with a global incidence rate that is gradually increasing. Exogenous insulin therapy cannot stably control blood sugar, and cannot prevent the occurrence and progression of diabetic complications

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  • Oligonucleotide for inhibiting blood-mediated inflammatory reaction in pancreatic islet transplantation
  • Oligonucleotide for inhibiting blood-mediated inflammatory reaction in pancreatic islet transplantation
  • Oligonucleotide for inhibiting blood-mediated inflammatory reaction in pancreatic islet transplantation

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Embodiment Construction

[0030] The present invention is described in further detail below through examples, but the content of the invention is not limited in any way. The antisense oligonucleotide sequences listed in Table 1 are representative molecules, and any other antisense oligonucleotide sequences that can inhibit TF expression Sense oligonucleotides can be used in the pharmaceutical composition of the present invention.

[0031] 1. Design, synthesis and modification of TF antisense oligonucleotides

[0032] Methods for designing antisense oligonucleotides include: (1) selecting an oligonucleotide that is adjacent to or overlapping the target region, (2) measuring the Gibbs free energy level of binding to the target gene, (3) analyzing the hybridization melting temperature (Tm), (4) Perform sequence library analysis to determine the region where the target site is located, for example, the 5' untranslated region, the translation initiation region, the 3' untranslated region and the translation...

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Abstract

The invention discloses an oligonucleotide for inhibiting a blood-mediated inflammatory reaction in pancreatic islet transplantation. The oligonucleotide comprises 7 to 75 nucleotides, at least comprises 3 three continuous achiral 5' to 3' nucleoside-triphosphate bonds, comprises a 5'-end untranslated region, a translation initiation region, a 3'-end untranslated region and / or a translation termination region which are complemented for a TF gene, and has the melting temperature of between 75 DEG C and 115 DEG C under the concentration of 1mmol. The oligonucleotide can be a ribose oligonucleotide or a deoxyribose oligonucleotide, can be modified chemically and can be used for preparing medicinal compositions for resisting the blood-mediated inflammatory reaction in the islet transplantation.

Description

technical field [0001] The invention relates to a class of oligonucleotides for inhibiting blood-mediated inflammatory response in pancreatic islet transplantation, in particular to the application of a class of antisense oligonucleotides for inhibiting the expression of tissue factor TF in the process of pancreatic islet transplantation. Background technique [0002] Diabetes is a serious metabolic disease with a global incidence rate that is gradually increasing. Exogenous insulin therapy cannot stably control blood sugar, and cannot prevent the occurrence and progression of diabetic complications. Establishing an endogenous insulin secretion system is a hotspot that people have been studying. Pancreatic islet transplantation brings hope of cure for patients with type 1 diabetes [Ridgway DM, White SA, Nicholson ML. Pancreatic islet cell transplantation: progress in the clinical setting treatments in endocrinology . 2003, 2(3): 173-189; White SA, James RF, Swift SM. Human i...

Claims

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Application Information

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IPC IPC(8): C12N15/113A61K48/00A61P29/00
Inventor 王维易受南孙仑泉
Owner 王维
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