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Antibodies that bind human dendritic and epithelial cell 205 (DEC-205)

A technology of DEC-205 and epithelial cells, applied in the field of human antibodies, can solve the problems of few dendritic cell reagents and few specific receptors of dendritic cells

Inactive Publication Date: 2010-11-17
CELLDEX THERAPEUTICS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Despite recent advances in the characterization of dendritic cells, little is known about dendritic cell-specific receptors such as DEC-205, and few dendritic cell-specific reagents are available

Method used

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  • Antibodies that bind human dendritic and epithelial cell 205 (DEC-205)
  • Antibodies that bind human dendritic and epithelial cell 205 (DEC-205)
  • Antibodies that bind human dendritic and epithelial cell 205 (DEC-205)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0325] Generation of DEC-205-specific human monoclonal antibody (HuMab)

[0326] by immunization with soluble human DEC-205 antigen A transgenic mouse ("HuMab" is a trademark of Medarex, Inc., Princeton, New Jersey) HC2 / KCo7 strain produced human anti-DEC-205 monoclonal antibodies. HC2 / KCo7 HuMAb mice were generated as described in US Patent Nos. 5,770,429 and 5,545,806, the entire disclosures of which are incorporated herein by reference.

[0327] Antigen and Immunization: Antigen is a soluble fusion protein comprising the extracellular domain of DEC-205 (containing all 10 lectin binding domains) fused to the Fc domain of an antibody. The nucleic acid and amino acid sequences of human DEC-205 are provided in PCT Patent Publication WO 96023882 (Steinman). Antigen was mixed with complete Freund's adjuvant (Sigma) for the first immunization. Afterwards, the antigen was mixed with incomplete Freund's adjuvant (Sigma). Additional mice were immunized with soluble DEC-205 prote...

Embodiment 2

[0332] Determination of HuMab affinity and rate constants by surface plasmon resonance (SPR)

[0333] Use Biacore TM 2000SPR instrument (Biacore AB, Uppsala, Sweden), according to the manufacturer's instructions, by Biacore TM Surface plasmon resonance (SPR) analysis examined the binding affinities and binding kinetics of various human anti-DEC-205 antibodies from Example 1.

[0334] Using the amine coupling kit provided by Biacore, according to the manufacturer's instructions (BIAcoreProduct No. BR-1000-50, including coupling reagents N-hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethyl Aminopropyl) carbodiimide hydrochloride (EDC)), the purified recombinant human DEC-205 fusion (or control) protein was covalently attached to Biacore using standard amine coupling chemistry TM On a CM5 sensor chip (carboxymethylated dextran covalently attached to a gold surface; Biacore Product No. BR-1000-14). Low levels of ligand are immobilized to limit any effect of analyte mass trans...

Embodiment 3

[0341] Binding of HuMab to cells expressing human DEC-205

[0342] The ability of anti-DEC-205 HuMabs to bind to DEC-205 on CHO-S cells expressing human DEC-205 on their surface was studied by flow cytometry as described below.

[0343] Antibody binding was detected to CHO-S cells expressing human DEC-205 on their surface. Protein A purified HuMabs 3D6-2F4, 3D6-4C8, 3G9-2D2, 5A8-1F1, 2D3-1F5-2A9, 3C7-3A3, 5D12-5G1, 1G6-1G6, and 3A4-1C10 with CHO expressing human DEC-205 -S cells and CHO-S control cells were incubated at 4°C. All antibodies were used at saturating concentrations. After 1 hr, with 0.1% BSA and 0.05% NaN 3 Cells were washed with PBS (PBA) and bound antibody was detected by incubating cells with a PE-labeled goat anti-human IgG Fc-specific probe at 4°C. Wash excess probe from cells with PBA, following manufacturer's instructions by using LSR TM Instrumental (BD Biosciences, NJ, USA) analysis determines cell-bound fluorescence. The results are shown in figur...

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Abstract

Isolated monoclonal antibodies which bind to human DEC-205 and related antibody-based compositions and molecules are disclosed. Also disclosed are pharmaceutical compositions comprising the antibodies, as well as therapeutic and diagnostic methods for using the antibodies.

Description

[0001] related application [0002] This application claims priority to US Provisional Application 61 / 002253, filed November 7, 2007, and US Provisional Application 61 / 191551, filed September 10, 2008, the contents of which are incorporated herein by reference. Background technique [0003] Dendritic cells (DCs) are specialized cells of the immune system. DCs have the unique ability to initiate primary and secondary T and B lymphocyte responses by presenting antigens in the form of peptides bound to cell surface major histocompatibility complex (MHC) molecules. The antigen-presenting function of dendritic cells has been associated with high-level expression of the human dendritic and epithelial cell 205 receptor (DEC-205) (Jiang et al. (1995) Nature 375(11) 151). [0004] DEC-205 is an endocytic receptor found primarily on dendritic cells, but also on B cells, brain capillaries, bone marrow stroma, intestinal villi and the epithelium of the lung airways, as well as the ectopl...

Claims

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Application Information

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IPC IPC(8): A61K39/395A61P35/00C07K16/28
CPCA61K47/48561C07K2319/00C07K16/2851C07K2317/92C07K2317/21C07K2317/77A61K2039/505A61P1/04A61P1/16A61P1/18A61P11/00A61P13/08A61P13/10A61P13/12A61P15/00A61P17/00A61P17/02A61P17/06A61P19/02A61P21/00A61P21/04A61P25/00A61P29/00A61P31/00A61P31/04A61P31/10A61P31/12A61P31/14A61P31/18A61P31/20A61P33/00A61P35/00A61P35/02A61P37/00A61P37/02A61P37/04A61P37/06A61P37/08A61P43/00A61P5/00A61P5/14A61P5/40A61P7/06A61P9/08A61P3/10
Inventor T·科勒L·何V·雷玛克里斯纳L·A·维塔勒
Owner CELLDEX THERAPEUTICS INC
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