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Anti-H3N8 subtype equine influenza virus monoclonal antibody and application thereof

A monoclonal antibody and influenza virus technology, applied in antiviral agents, antiviral immunoglobulins, antibodies, etc., can solve problems such as losses in horse breeding and horse racing industries

Inactive Publication Date: 2010-11-24
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Like other influenza viruses, equine influenza A virus spreads rapidly and often presents as an explosive epidemic, which has brought certain losses to both the horse breeding and racing industries

Method used

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  • Anti-H3N8 subtype equine influenza virus monoclonal antibody and application thereof
  • Anti-H3N8 subtype equine influenza virus monoclonal antibody and application thereof
  • Anti-H3N8 subtype equine influenza virus monoclonal antibody and application thereof

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Embodiment 1

[0012] The present invention will be further described below with reference to specific embodiments, and the advantages and characteristics of the present invention will become clearer with the description. However, these examples are only exemplary and do not constitute any limitation to the scope of the present invention. It should be understood by those skilled in the art that the details and forms of the technical solutions of the present invention can be modified or replaced without departing from the spirit and scope of the present invention, but these modifications and replacements all fall within the protection scope of the present invention. Example 1 Preparation of hybridoma cell lines secreting anti-H3N8 subtype equine influenza virus monoclonal antibodies

[0013] 1. Materials and methods

[0014] 1.1 Experimental materials

[0015] 1.1.1 Main reagents

[0016] HAT, HT selection medium, HRP-goat anti-mouse IgG, Freund's complete adjuvant, and Freund's incomplete...

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PUM

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Abstract

The invention discloses an anti-H3N8 subtype equine influenza virus monoclonal antibody and an application thereof. The anti-H3N8 subtype equine influenza virus monoclonal antibody is secreted by hybridoma cell strains with the microbial collection number of CGMCC No.3543. EL1SA, and Western-bolt methods prove that the monoclonal antibody of the invention has no cross reaction with equine infectious anemia virus, equine arteritis virus and normal chick embryo allantois fluid; and the abdominal dropsy ELISA valence of the H3N8 / 10 of the invention is 1:1.6*104. The anti-H3N8 subtype equine influenza virus monoclonal antibody of the invention can be applied to antigenicity analysis on A-type equine influenza virus, serodiagnosis, vaccine quality monitoring, epidemiological survey and other aspects.

Description

technical field [0001] The present invention relates to an equine influenza virus monoclonal antibody, in particular to an anti-H3N8 subtype equine influenza virus monoclonal antibody and a hybridoma cell strain secreting the monoclonal antibody. The use of the virus belongs to the field of equine influenza virus monoclonal antibodies. Background technique [0002] Equine influenza (EI), referred to as equine influenza, is an acute fulminant infection of equine animals caused by equine influenza A virus (EIV) of the genus Influenzavirus of the family Orthomyxoviridae (Orthomyxoviridae). sick. Internationally, according to the difference of H and N of influenza virus, H is divided into 15 types, and N has 8 subtypes. There are only two subtypes that infect horses: vest I and vest II, which belong to the H7N7 and H3N8 subtypes, respectively. [0003] Like other influenza viruses, equine influenza A virus spreads rapidly, often in an explosive fashion, which has brought cert...

Claims

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Application Information

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IPC IPC(8): C12N5/20C07K16/10A61K39/42A61P31/16G01N33/577C12R1/91
Inventor 郭巍相文华才琳赵立平闫妍黄文强
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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