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Method for identifying isatis root in Chinese medicinal preparations

A technology of traditional Chinese medicine preparation and Banlangen, which is applied in the direction of medical formula, antiviral agent, drug combination, etc. It can solve the problems of insufficient specificity, poor color rendering effect of thin layer, and unclear spots, etc.

Inactive Publication Date: 2010-12-08
北京九草堂药物研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of this method are: 1. Because amino acids are broad-spectrum components, almost all plant extracts contain amino acid components. Taking one of the amino acid components as a comparison, it is obvious that the specificity is not strong enough; especially in the identification of compound preparations, Components of other medicinal materials are likely to interfere with the identification results
2. In actual operation, it is found that when ninhydrin is used as the chromogenic agent, the color development effect of the thin layer is not good, the spots are not clear enough, and false negative results are prone to occur

Method used

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  • Method for identifying isatis root in Chinese medicinal preparations

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] TLC Identification of Example 1 Radix Radix Granules

[0016] Take 20g of Banlangen granules from a factory in Beijing, add 20ml of water to dissolve, extract 3 times with an equal volume of n-butanol, collect the n-butanol layer, evaporate to dryness, add 2ml of methanol to dissolve the residue to obtain the test solution. Take 2g of Radix Radix as reference medicinal material, add 50ml of water, decoct 3 times for 1 hour each time, filter, concentrate the filtrate to 20ml, extract 3 times with an equal volume of n-butanol, collect the n-butanol layer, evaporate to dryness, add 2ml of methanol to the residue Dissolved to obtain the reference medicinal material solution.

[0017] Thin-layer plate: silica gel G plate; developer: ethyl acetate-ethanol-water (7:2:1); developer: 1% p-dimethylaminobenzaldehyde in 30% sulfuric acid solution, heated to spots at 60°C Color development is clear.

[0018] Results: In the chromatogram of the test product, spots of the same color...

Embodiment 2

[0019] Example 2 TLC Identification of Radix Isatidis in Cangzhu Tongshen Oral Liquid

[0020] Preparation of the test solution: get three batches of this product (1002007, 1002008, 1002009) each 20ml, get this product 20ml, extract 3 times with an equal volume of water-saturated n-butanol, collect the n-butanol layer, evaporate to dryness, and the residue Add 5ml of methanol to dissolve to obtain the test solution.

[0021] Preparation of blank control solution: prepare negative Cangzhu Tongshen oral liquid sample without Radix Isatidis according to the prescription, take 20ml, and the preparation method is the same as that of the test solution to obtain the blank control solution.

[0022] Preparation of the reference medicinal material solution: take 2 g of the Radix Radix reference medicinal material, add 50 ml of water, decoct 3 times for 1 hour each time, filter, concentrate the filtrate to 20 ml, extract 3 times with an equal volume of water-saturated n-butanol, and col...

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Abstract

The invention discloses a method for identifying isatis root in Chinese medicinal preparations, belongs to a Chinese medicament detecting method, in particular to qualitative identification of the isatis root in the Chinese medicinal preparations. In the method, a thin layer chromatography is adopted, the sample is extracted with n-butyl alcohol and concentrated to fulfill the aim of component enrichment, an ethyl acetate-ethanol-water developing system is used for developing, and sulfuric acid solution of paradime thylaminobenzaldehyde is sprayed on the sample for developing so as to obtain a chromatogram which has clear and complete spots and is blank without interference. The method is accurate and effective, has high specificity, and can be used for qualitative identification of the isatis root in the Chinese medicinal preparations.

Description

technical field [0001] The invention belongs to a traditional Chinese medicine detection method, and in particular relates to the qualitative identification of Radix isatidis in traditional Chinese medicinal preparations. Background technique [0002] Radix Isatidis is the dried root of Isatis indigotica Forti. It was first recorded in "Shen Nong's Materia Medica" and listed as top grade. "Chinese Pharmacopoeia" has been included in the 1985 edition to the 2005 edition. It has the effects of clearing away heat and detoxification, cooling blood and relieving throat, and is widely used clinically in the treatment of various diseases such as influenza, mumps, Japanese encephalitis, hepatitis, sore throat, pink eye, and chickenpox. Because of its wide range of clinical uses, it is used in many preparations, such as Compound Banlangen Granules, Banlangen Tablets, Haolan Ganmao Granules, Qingkailing Oral Liquid, etc. In the 2005 edition of "Chinese Pharmacopoeia", the identific...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/315G01N30/90A61P31/16A61P31/14A61P31/20A61P1/16A61P11/04A61P27/02A61P17/00A61P31/22A61K125/00
Inventor 雷思琦许冠英白景玲陈丽平
Owner 北京九草堂药物研究院有限公司