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Application of Na+/H+ reverse transport protein and protein-coding gene thereof in culturing disease-resistant transgenic plant

An antiporter, encoding gene technology, applied in the introduction of foreign genetic material using a vector, recombinant DNA technology, etc., can solve the problem that few studies focus on the NHX1 gene, etc.

Inactive Publication Date: 2012-05-30
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although many reports have demonstrated that NHX1 has a salt-tolerance function in plants, few studies have focused on other functions of the NHX1 gene, especially the role of this gene in biotic stress

Method used

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  • Application of Na+/H+ reverse transport protein and protein-coding gene thereof in culturing disease-resistant transgenic plant
  • Application of Na+/H+ reverse transport protein and protein-coding gene thereof in culturing disease-resistant transgenic plant
  • Application of Na+/H+ reverse transport protein and protein-coding gene thereof in culturing disease-resistant transgenic plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1. Resistance experiment of transgenic tobacco

[0029] The acquisition of plant material

[0030] 1. Obtain wild-type tobacco, empty vector tobacco and transgenic tobacco

[0031] plant material:

[0032] The wild-type tobacco variety 'Wisconsin 38' (Nicotiana tabacum cv 'Wisconsin 38') (purchased from the Tobacco Seed Resources Bank of the Tobacco Research Institute of the Chinese Academy of Agricultural Sciences) is represented by WT.

[0033] Transgenic tobacco converts Na + / H + Antiporter protein coding gene SeNHX1 transformed wild-type tobacco 'Wisconsin 38' ("W38") obtained (Zhou SF, Chen XY, Zhang XG, Li YX (2008) Improved salt tolerance in tobacco plants by co-transformation of a betaine synthesis gene BADH and a vacuolar Na + / H + antiporter gene SeNHX1. Biotechnol Lett 30:369-376). Wherein, the SeNHX1 gene uses the following primers 1 and 2 as primers, uses the first-strand cDNA synthesized from the RNA of Salicornia as a template, and PCR am...

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Abstract

The invention discloses application of Na+ / H+ reverse transport protein and a protein-coding gene thereof in culturing a disease-resistant transgenic plant, wherein an amino acid sequence of the Na+ / H+ reverse transport protein is as shown in Genbank No. AAN08157; and a nucleotide sequence of the protein-coding gene is as shown in Genbank No. AY131235. The Na+ / H+ reverse transport protein and theprotein-coding gene thereof can be used for improving disease resistance, salt tolerance, oxidative stress resistance and systemic acquired resistance of the plant.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to Na + / H + Application of antiporter and its coding gene in breeding disease-resistant transgenic plants. Background technique [0002] Due to the immobile nature of plants, various mechanisms have been developed in the course of evolution to adapt and respond to various environments and stresses with their own biochemical and physiological adjustments. Among them, no matter in halophytes or sweet soil plants, there is a conserved ability of vacuoles to compartmentalize sodium ions; this process includes: through the Na on the tonoplast membrane + / H + Antiporter, which partitions the sodium ions in the cytoplasm into the vacuole, thereby reducing the toxicity of toxic sodium ions to various enzymes in the cytoplasm (Blumwald E(2000) Sodium transport and salt tolerance implants.Curr Opin Cell Biol. 12:431-434). [0003] To date, researchers have cloned Na on the tonoplast membran...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82
Inventor 李银心陈显扬
Owner INST OF BOTANY CHINESE ACAD OF SCI
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