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Method for improving yield of wuxistatin

A high-yield, statin technology, applied in the field of statin production in Wuxi, can solve the problem of low conversion rate of substrates, achieve the effect of shortening fermentation time and broad application prospects

Inactive Publication Date: 2010-12-08
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The conversion conditions were kept constant throughout, and the final substrate conversion was not high

Method used

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  • Method for improving yield of wuxistatin
  • Method for improving yield of wuxistatin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Amycolatopsi sp.CGMCC NO.1149 was transferred to the seed medium (50 mL in a 250 mL Erlenmeyer flask), and cultured statically at 28°C for 48 hours. The seed liquid is filtered through two layers of lens-cleaning paper to remove the mycelium, and the collected filtrate is the spore seed liquid. Inoculate the transformation medium according to 10% of the inoculum amount, and culture on a rotary shaker at 150 r / min at 30° C. for 48 hours to prepare the transformation medium. The pH of the transformation culture medium was adjusted to 7.5, and lovastatin solution was added to the transformation culture medium at a concentration of 0.8 g / L. After 20 hours of transformation, the fermentation broth was centrifuged at 8000 rpm at 4° C., and the supernatant was taken.

[0023] After fully soaking the macroporous adsorption resin with an appropriate amount of 95% ethanol for 24 hours, rinse with 95% ethanol until the effluent is not turbid after adding water (1:5), and then wash...

Embodiment 2

[0033] Phase 1 pH is 7.0, press, add lovastatin solution at a concentration of 1.5g / L.

[0034] The pH of stage two is 5.5, and the purified product I is added at a concentration of 0.6 g / L.

[0035] Other conditions are the same as in Example 1. After the fermentation is finished, the content of Wuxi statin is measured, and the yield is 15% higher than that of the comparative technical method.

Embodiment 3

[0037] Phase 1 pH is 7.5, and lovastatin solution is added at a concentration of 1.2 g / L.

[0038] The pH of stage two is 6.0, and the purified product I is added at a concentration of 0.6 g / L.

[0039] Other conditions are the same as in Example 1. After the fermentation is finished, the content of Wuxi statin is measured, and the yield is 13% higher than that of the comparative technical method.

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Abstract

The invention discloses a method for improving yield of wuxistatin and belongs to the field of fermentation engineering. By using Amycolatopsis sp. with the collection number of CGMCC NO.1149) as a production strain, and optimizing the fermentation condition through two-stage pH control, the method improves the yield of the wuxistatin by 16 percent, shortens the fermentation time simultaneously, and has wide application prospect.

Description

Technical field: [0001] The invention relates to a method for producing Wuxi statin, in particular to a method for increasing the yield of Wuxi statin by staged pH control, and belongs to the technical field of fermentation engineering. Background technique: [0002] With the improvement of living standards and the adjustment of dietary structure, the number of patients with hyperlipidemia and coronary heart disease is increasing. Cardiovascular disease is a kind of disease that seriously endangers human health. Its morbidity and mortality rate remain high worldwide, accounting for nearly 1 / 4 of the total death of the world's population. It is the number one killer of human health. [0003] Statins belong to hydroxymethylglutaryl coenzyme A (HMG-CoA) reductase inhibitors, which are the first choice of lipid-lowering drugs and one of the hot research topics at home and abroad. This experiment discloses a method for producing Wuxi statin in the patent No.: 200410044893.6, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/06C12R1/01
Inventor 诸葛斌牟琳方慧英诸葛健
Owner JIANGNAN UNIV