Lactobacillus plantarum strain and application thereof

A technology of Lactobacillus plantarum and strains, applied in the directions of bacteria, microorganisms, microorganism-based methods, etc., can solve problems such as difficulty in large-scale production and difficulty in culturing

Inactive Publication Date: 2010-12-15
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, rumen bacteria are strictly anaerobic bacteria, difficult to cultivate, and difficult to achieve large-scale production

Method used

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  • Lactobacillus plantarum strain and application thereof
  • Lactobacillus plantarum strain and application thereof
  • Lactobacillus plantarum strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0014] Isolation and purification of strains

[0015] Separation and screening of strains were carried out from pickles purchased from different supermarkets in Hangzhou city. The specific operation was to wash the pickles with sterile water, and the washing liquid was diluted with 0.8% sterile saline gradient (10% by weight) -5 ), and then coated on the MRS plate, all the plates were divided into 8 groups, placed at different temperatures (30°C or 37°C) and different oxygen levels (anaerobic or microaerobic) and cultured for different time (24 or 48 hours) , Pick a single colony and insert it into the MRS liquid medium, culture it under the same conditions as the isolation conditions, and store it in the MRS slant medium.

[0016] After activation, the strains isolated above were inoculated in MRS liquid medium containing 0.2mg / ml LA, cultured at 180rpm for 24h, and CLA was extracted with twice the volume of n-hexane, and then the synthetic amount of CLA in the medium was mea...

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Abstract

The invention discloses a high-yielding CLA (conjugated linoleic acid) lactobacillus plantarum strain, which is named as 'Lactobacillus plantarum' lp15-2-1 and collected in China General Microbiological Culture Collection Center (CGMCC) (collection date: April 27th, 2010; collection NO.: CGMCC NO. 3782). The strain of the invention can convert linoleic acid into conjugated linoleic acid, wherein the maximum yield of the conjugated linoleic acid in resting cells is 1,023.55 mu g/ml, and the conversion rate of the linoleic acid by direct shaking culture reaches 21.32 percent; therefore, the strain of the invention can be applied to the industrial production of acidophilous milk, pickles, fruit drinks and the like.

Description

technical field [0001] The invention belongs to the technical field of food microbial fermentation, and in particular relates to a strain of Lactobacillus plantarum and its application. Background technique [0002] Conjugated linoleic acid (CLA) is octadecadienoic acid containing cis- or trans-conjugated double bonds. It is a group of positions and Generic term for conformational isomers. Among them, the two isomers of CLA, cis 9, anti 11-CLA (c9, t11-CLA) and anti 10, cis 12-CLA (t10, c12-CLA) have important physiological functions, such as anticancer (colon cancer, Gastric cancer, breast cancer, prostate cancer), improve cellular immunity, reduce body fat content, prevent the occurrence of diabetes and inhibit atherosclerosis, etc. [0003] In nature, CLA is mainly derived from rumen animal meat and dairy products, and the content of CLA varies with animal species, feeding methods and feeding conditions; the content of CLA in human milk is 0.29% to 0.71%, and that in ho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/64C12R1/25
Inventor 何国庆陈启和张强阮晖傅明亮
Owner ZHEJIANG UNIV
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