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Mesenchymal stem cell cryopreserving liquid directly applied to veins

A technique for mesenchymal stem cells and cryopreservation solution, applied in the field of mesenchymal stem cell cryopreservation solution and its preparation, can solve the problems of potential safety hazards, high price, differences in cell preparation conditions and characteristics, etc.

Active Publication Date: 2010-12-22
青岛奥克生物开发有限公司
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AI Technical Summary

Problems solved by technology

At present, mesenchymal stem cells have been widely used in clinical research and treatment at home and abroad. A course of treatment for most diseases will last for 1-2 months, and cells will be infused more than once in each course of treatment. The mesenchymal stem cells are infused once every time. Therefore, if the mesenchymal stem cells are re-prepared before each application, there will inevitably be some differences in the preparation conditions and characteristics of the cells used each time.
[0010] In addition, the active ingredients in the cryopreservation solution in the prior art are cell culture medium and fetal bovine serum, etc., which, in addition to potential safety hazards when applied to the human body, are also expensive, and the quality of reagents from different manufacturers and different production batches varies, so there are It is necessary to study a cheaper, more convenient and safer mesenchymal stem cell cryopreservation medium

Method used

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  • Mesenchymal stem cell cryopreserving liquid directly applied to veins
  • Mesenchymal stem cell cryopreserving liquid directly applied to veins

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0023] 1. Preparation of human AB (type) plasma for cryopreservation

[0024] (1) Human AB blood (purchased from Qingdao Central Blood Center) containing 2.5% sodium citrate by mass (may not be included, does not affect the technical effect of the present invention) is placed in a sterile centrifuge tube with a capacity of 50 ml, Centrifuge at 1000rpm for 15 minutes;

[0025] (2) Aspirate the supernatant, transfer it into a new centrifuge tube, and centrifuge at 4000g for 20 minutes to remove platelets. The supernatant is human AB plasma for cryopreservation, which is used in the following 2 steps.

[0026] 2. Preparation of mesenchymal stem cell cryopreservation medium

[0027] Solution I: DMSO (dimethyl sulfoxide, purchased from SIGMA), human AB plasma for cryopreservation, and Bomaili A injection (purchased from Shanghai Baite) were mixed uniformly at a volume ratio of 1:3:6, and placed at 4°C spare;

[0028] Solution II: DMSO, fetal bovine serum (purchased from GIBCO), ...

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Abstract

The invention relates to a mesenchymal stem cell cryopreserving liquid directly applied to veins and a preparation method. The cryopreserving liquid does not contain fetal calf serum, thereby not only having low price, but also being directly applied to intravenous injection, and having safety in clinical practice. The cryopreserving liquid of the invention can maintain more than 90% of mesenchymal stem cells to survive for more than two months and meet the demand that most of mesenchymal stem cells are required to be cured in a course of the treatment.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a mesenchymal stem cell cryopreservation solution which can be directly used intravenously and a preparation method thereof. technical background [0002] Mesenchymal stem cells (MSCs) are a group of mesoderm-derived pluripotent stem cells with self-renewal and multi-directional differentiation potential, which can be found in bone marrow, placenta, umbilical cord, amniotic membrane, muscle, fetal liver, cord blood and other tissues widely exist. Mesenchymal stem cells not only have the ability to differentiate in multiple directions, but also have the functions of supporting hematopoiesis and regulating immunity. Because of their advantages such as wide sources, convenient material collection, simple and rapid in vitro expansion, and unchanged differentiation ability after expansion and culture, they are widely used in clinical practice. It has broad application...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 魏斯溧高宏王丽胡建霞张学峰张美荣
Owner 青岛奥克生物开发有限公司
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