L-arginine producing strain corynebacterium glutamicum and preparation method and application

Abstract

The invention discloses L-arginine producing strain corynebacterium glutamicum ZD2009, CCTCC M209260 and a preparation method and application. The preparation method comprises the following steps of: A, performing ultraviolet mutagenesis, namely culturing the corynebacterium glutamicum, centrifuging, removing supernatant, washing, and scattering the strain by using glass beads to obtain cell suspension; B, performing 1-methyl-3-nitro-1-nitrosoguanidine mutagenesis, namely culturing the corynebacterium glutamicum, centrifuging, removing the supernatant, washing, scattering the strain by using the glass beads, washing with phosphate buffer after washing, adding 1-methyl-3-nitro-1-nitrosoguanidine for mutagenesis, washing with the phosphate buffer to remove the 1-methyl-3-nitro-1-nitrosoguanidine and stopping the mutagenesis; C, determining shake flask fermentation conditions; D, determining a fermentation medium and inoculation amount; E, determining pH value; and F, determining liquid loading amount. The acid producing amount of the L-arginine producing strain corynebacterium glutamicum reaches 44g / L; and the strain produces the acid stably, has high genetic stability, reduces production cost and improves efficiency.

Description

technical field [0001] The present invention relates to the field of biotechnology, more specifically to an L-arginine-producing bacterium Corynebacterium glutamicum, to a preparation method of the L-arginine-producing bacterium Corynebacterium glutamicum, and to L-arginine Use of the producing bacterium Corynebacterium glutamicum in animal feed. Background technique [0002] L-arginine is the final product of the arginine biosynthesis pathway, a semi-essential basic amino acid for humans and animals, and an essential amino acid for young animals. L-arginine has a wide range of uses. In the pharmaceutical industry, L-arginine is an important intermediate metabolite in the urea cycle of organisms. It is also one of the main components of compound amino acid infusion, or it is used to prepare nutrition or special treatment supplies. It is widely used as an antidote for ammonia poisoning hepatic coma and a promoter of liver function. It has a remarkable effect on viral hepatit...

AI Technical Summary

Problems solved by technology

At present, the domestic research on the production of L-arginine by fermentation is limited to the laboratory level, which is far behind the international technology. Therefore, it is of great significance to breed high-yielding strains of L-arginine and optimize its fermentation process.

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