Porphyrin detection method based on self-assembly monomolecular film

A technology of self-assembly of single molecules and detection methods, which is applied in measurement devices, analysis materials, material analysis by electromagnetic means, etc. Low cost, high sensitivity, simple operation effect

Inactive Publication Date: 2011-01-05
NORTHWEST NORMAL UNIVERSITY
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Problems solved by technology

The traditional detection methods of porphyrin are: spectrophotometry, fluorescence method, high performance liquid chromatography, mass spectrometry, chromatography-mass spectrometry, thin layer chromatography, capillary electrophoresis separation-fluorescence detec...

Method used

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  • Porphyrin detection method based on self-assembly monomolecular film
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  • Porphyrin detection method based on self-assembly monomolecular film

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Embodiment Construction

[0031] The water used in the experiment process was double distilled water, and the reagents used in the experiment were all analytically pure. The experiments were all carried out at room temperature.

[0032] Instruments and reagents used:

[0033] A multi-channel electrochemical workstation (VMP2, Princeton Instruments, USA) was used for AC impedance experiments; a quartz tube heated automatic double pure water still (1810B, Shanghai Asia Pacific Technology Glass Co., Ltd.) was used for distilling twice distilled water; an electronic balance (Beijing Sai Doris Instrument Co., Ltd.) is used to weigh medicine; UV-1102 ultraviolet-visible spectrophotometer (Shanghai Tianmei Scientific Instrument Co., Ltd.) is used to measure the ultraviolet absorption spectrum of porphyrin solution; ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd. ); Aluminum oxide grinding powder (0.30 μm, 0.05 μm, Shanghai Chenhua Instrument Reagent Co., Ltd.) was used to process the gold electro...

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Abstract

The invention relates to a porphyrin detection method based on a self-assembly monomolecular film. The method comprises the following steps of: firstly, dipping a phosphate modified electrode into a dichloromethane methylene solution of porphyrin with different concentrations for reaction and observing the change of porphyrin solution colors instantly; and eluting the electrode after each reaction, and then carrying out electrochemistry impedance scanning in a potassium ferricyanide probe molecule-containing potassium chloride electrolyte solution by using a three-electrode system, wherein the charge transfer impedance value (Rct) of the monomolecular self-assembly film and a logarithm (lg C) of the tetraphenyl porphyrin concentration are in a favorable linear relation. In the invention, the porphyrin is detected by combining a colorimetry and an AC impedance method, the porphyrin detection method has convenience and speediness, high sensitivity, simple operation and low cost.

Description

technical field [0001] The invention relates to a porphyrin detection method, in particular to a porphyrin detection method based on a self-assembled monomolecular film. Background technique [0002] Porphyrin and its metal complexes are favored by researchers due to their excellent photoelectric properties and stability, and are a current research hotspot. There are many kinds of porphyrins with different structures, which widely exist in nature, such as myoglobin, hemoglobin, cytochrome P-450, iron porphyrin in peroxidase, magnesium porphyrin in chlorophyll, vitamin B 12 The cobalt porphyrins in them are closely related to life activities and play an important role in the electron transfer process of living organisms. With the synthesis and in-depth study of macrocyclic compounds such as porphyrins and metalloporphyrins, they play an increasingly important role in the research of biomimetic membranes, catalysis, solar energy utilization, medicine and analytical chemistry....

Claims

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Application Information

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IPC IPC(8): G01N27/416
Inventor 卢小泉赵东霞张翠忠王文婷忤博万陕多亮史海材
Owner NORTHWEST NORMAL UNIVERSITY
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