Porphyrin detection method based on self-assembly monomolecular film

Abstract

The invention relates to a porphyrin detection method based on a self-assembly monomolecular film. The method comprises the following steps of: firstly, dipping a phosphate modified electrode into a dichloromethane methylene solution of porphyrin with different concentrations for reaction and observing the change of porphyrin solution colors instantly; and eluting the electrode after each reaction, and then carrying out electrochemistry impedance scanning in a potassium ferricyanide probe molecule-containing potassium chloride electrolyte solution by using a three-electrode system, wherein the charge transfer impedance value (Rct) of the monomolecular self-assembly film and a logarithm (lg C) of the tetraphenyl porphyrin concentration are in a favorable linear relation. In the invention, the porphyrin is detected by combining a colorimetry and an AC impedance method, the porphyrin detection method has convenience and speediness, high sensitivity, simple operation and low cost.

Description

technical field [0001] The invention relates to a porphyrin detection method, in particular to a porphyrin detection method based on a self-assembled monomolecular film. Background technique [0002] Porphyrin and its metal complexes are favored by researchers due to their excellent photoelectric properties and stability, and are a current research hotspot. There are many kinds of porphyrins with different structures, which widely exist in nature, such as myoglobin, hemoglobin, cytochrome P-450, iron porphyrin in peroxidase, magnesium porphyrin in chlorophyll, vitamin B 12 The cobalt porphyrins in them are closely related to life activities and play an important role in the electron transfer process of living organisms. With the synthesis and in-depth study of macrocyclic compounds such as porphyrins and metalloporphyrins, they play an increasingly important role in the research of biomimetic membranes, catalysis, solar energy utilization, medicine and analytical chemistry....

AI Technical Summary

Problems solved by technology

The traditional detection methods of porphyrin are: spectrophotometry, fluorescence method, high performance liquid chromatography, mass spectrometry, chromatography-mass spectrometry, thin layer chromatography, capillary electrophoresis separation-fluorescence detection method, fluorescence narrow line method, magnetic circle Two-color method and other methods, but these methods are not fast and simple enough, and the operation and maintenance of the instrument system are more complicated, and the cost of testing is relatively high.

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