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Method for extracting effective ingredient of tobacco leaf from waste tobacco

A technology of waste tobacco leaves and tobacco leaves, which is applied in the fields of tobacco, tobacco preparation, application, etc., can solve the problem of low extraction rate of active ingredients, and achieve the effects of rich active ingredients, reduced water consumption, and saving raw materials

Active Publication Date: 2012-09-19
CHINA TOBACCO GUANGDONG IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the problem that the extraction rate of effective components in waste tobacco leaves is not high, the present invention provides a method for extracting effective components of tobacco leaves from waste tobacco leaves, which greatly improves the extraction efficiency of volatile components in waste tobacco leaves

Method used

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  • Method for extracting effective ingredient of tobacco leaf from waste tobacco
  • Method for extracting effective ingredient of tobacco leaf from waste tobacco
  • Method for extracting effective ingredient of tobacco leaf from waste tobacco

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 extracts active ingredient in waste tobacco leaves with the method provided by the invention

[0029] The raw material waste tobacco leaves were hand-cut into shreds, dried in an oven at 40°C for 5 hours, crushed, left overnight, and stored in sealed bags for later use.

[0030] Take 30g of shredded tobacco leaves and place in a 500mL conical flask, add 300mL of citric acid-sodium citrate buffer solution with a pH value of 5.4. Then add 0.04g compound plant hydrolase, 0.08g cellulase, and 0.04g neutral protease. The above three enzymes were all purchased from Novozymes (China) Biotechnology Co., Ltd. Shake and react on an air-bath shaker at 45°C for 5 hours, filter, collect the filtrate, add 100mL of water to the filter residue, shake and extract at room temperature for 20min to obtain the extract, combine the extract and the extract, concentrate to 25mL by rotary evaporation under reduced pressure, and obtain waste tobacco leaves Concentrate the extract. ...

Embodiment 2

[0031] Embodiment 2 extracts effective constituents in waste tobacco leaves with the method provided by the invention

[0032] The raw material waste tobacco leaves were shredded manually, dried in an oven at 40°C for 5 hours, crushed, left overnight, and stored in a sealed bag for later use.

[0033]Take 40g of shredded tobacco leaves and place in a 500mL conical flask, add 200mL of disodium hydrogen phosphate-potassium dihydrogen phosphate buffer solution with a pH value of 4.6. Then add 0.02g compound plant hydrolase, 0.04g cellulase, and 0.02g neutral protease. The above three enzymes were all purchased from Novozymes (China) Biotechnology Co., Ltd. Shake and react on an air bath shaker at 50°C for 7 hours, filter, collect the filtrate, add 80mL of water to the filter residue, shake and extract at room temperature for 40min to obtain the extract, combine the extract and the extract, concentrate to 25mL by rotary evaporation under reduced pressure, and obtain waste tobacco ...

Embodiment 3

[0034] Embodiment 3 extracts active ingredient in waste tobacco leaf with the method provided by the invention

[0035] The raw material waste tobacco leaves were hand-cut into shreds, dried in an oven at 40°C for 5 hours, crushed, left overnight, and stored in sealed bags for later use.

[0036] Take 40g of shredded tobacco leaves and place in a 500mL conical flask, add 160mL of acetic acid-sodium acetate buffer solution with a pH value of 4.8. Then add 0.04g compound plant hydrolase, 0.08g cellulase, and 0.04g neutral protease. The above three enzymes were all purchased from Novozymes (China) Biotechnology Co., Ltd. Shake and react on an air bath shaker at 30°C for 8 hours, filter, collect the filtrate, add 60mL of water to the filter residue, shake and extract at room temperature for 60min to obtain the extract, combine the extract and the extract, concentrate to 25mL by rotary evaporation under reduced pressure, and obtain waste tobacco leaves Concentrate the extract.

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Abstract

The invention relates to the tobacco processing field and discloses a method of extracting active components from waste tobacco. The method comprises the steps of reacting waste tobacco with composite plant hydrolase accounting for 0.05-3 wt% of waste tobacco, 0.05-3 wt% of cellulase and 0.05-3 wt% of neutral proteinase in a buffer solution with the pH value of 4.5-6.0 for 4-8 hours, wherein the volume-to-mass ratio by mL / g of buffer solution to waste tobacco is 5-10:1; filtering after the reaction, taking the filter residues, and adding water for reextraction, wherein the volume-to-mass ratio by mL / g of water to waste tobacco is 2-4:1; and mixing the extracts. When the extraction yield and extract purity in the method and in the prior extraction method are measured, the result indicates that the method enhances the extraction efficiency by 25.2%. Meanwhile, the method increases the content of volatile components by 74.2% as compared with the conventional method and increases the content of main fragrance component neophytadiene by 6 times. The method has the advantages of low water consumption, low reaction temperature and simple operation and lowers the production cost.

Description

technical field [0001] The invention relates to the field of tobacco processing, in particular to a method for extracting effective components of tobacco leaves from waste tobacco leaves. Background technique [0002] The planting area of ​​tobacco plants in my country ranks first in the world, and the tobacco planting and product processing industry plays an important role in the development of the national economy. At present, tobacco grown at home and abroad is mainly used to make cigarette products. As a big tobacco country, in the process of growing and purchasing tobacco for cigarettes, there are about 200,000 tons of waste tobacco leaves in the country every year, accounting for about 13% of the total tobacco output in the country. These waste leaves are treated as waste, causing waste and pollution. , the foreign tobacco industry is facing the same situation. [0003] The disorderly flow of unused waste tobacco leaves has brought hidden dangers to the normal tobacc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A24B3/14
Inventor 周瑢孔浩辉胡静李峰韩冰
Owner CHINA TOBACCO GUANGDONG IND