Method for inducing regeneration plant of tetraena mongolica by somatic cell embryo

A technology of quadrangle wood and induction medium, applied in the biological field, can solve the problems of low seed setting rate and high embryo abortion rate

Inactive Publication Date: 2011-01-26
INST OF BOTANY CHINESE ACAD OF SCI
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  • Abstract
  • Description
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Problems solved by technology

Tetragalus mainly reproduces sexually, but only 11% of the plants can flower normally, the seed setting rate is low, and the embryo abortion rate is high
However, there is no report on a technical system suitable for large-scale and rapid propagation of Tetragalus, and the induction of regeneration of complete plants through the somatic embryo pathway.

Method used

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  • Method for inducing regeneration plant of tetraena mongolica by somatic cell embryo
  • Method for inducing regeneration plant of tetraena mongolica by somatic cell embryo
  • Method for inducing regeneration plant of tetraena mongolica by somatic cell embryo

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Embodiment 1

[0042]1. Callus induction and subculture

[0043] 1. Tetraena mongolica seeds (documents that record this material: Wang, G., Q.Q.Lin, et al. (2007). "Tetraena mongolica Maxim can accumulate large amounts of triacylglycerol in phloem cells and xylem parenchyma of stems." Phytochemistry 68(15): 2112-2117.; the public can obtain from the Institute of Botany, Chinese Academy of Sciences) Selection: Considering the high abortion rate of Tetraphyllum seeds, select seeds with embryos and plumpness, and use After soaking in sterile water with 1% Tween for 10 minutes, disinfect with 70% ethanol for 1 minute, soak in 10% sodium hypochlorite for 6-7 minutes, and rinse with sterile water for 4-5 times. Blot the water on the surface of the seeds with sterile filter paper, and inoculate them on 1 / 2MS medium without hormones. From the third day, the seeds will germinate. After about 20-30 days, sterile seedlings will be formed. Take the young stems Do the following experiment.

[0044] ...

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Abstract

The invention discloses a method for inducing a regeneration plant of tetraena mongolica by a somatic cell embryo. The method comprises the following steps of: 1) inducing the cut stem of the tetraena mongolica to form a callus; 2) inoculating the callus obtained in the step 1 into an embryonic callus induction medium to form an embryonic callus; 3) performing maturation and germination culture on the callus obtained in the step 2 to form a sprout; and 4) inoculating the sprout of the step 3 into an elongation and rooting medium to obtain a complete regeneration plant. The research induces the somatic cell embryo so as to form the regeneration plant by taking the callus of the tetraena mongolica as an explant, lays a foundation for establishing a high-efficiency and stable somatic embryogeny system, and has very import significance to the researches in the aspects such as rapid propagation, gene engineering, genetic transformation, genetic improvement and the like of the tetraena mongolica.

Description

technical field [0001] The invention relates to a method for inducing regenerated plants of Sihemu by somatic embryos, and belongs to the field of biotechnology. Background technique [0002] Tetraena mongolica (Tetraena mongolica Maxim), a small deciduous shrub and strong xerophyte, is a relic species of the ancient Mediterranean 140 million years ago. It is mainly distributed between Bayangol in the west of Inner Mongolia Autonomous Region and Shizuishan Mountain at the eastern foot of Helan Mountain in Ningxia Hui Autonomous Region. about 10,000 hectares. Tetragalus mainly reproduces sexually, but only 11% of the plants can flower normally, the seed setting rate is low, and the embryo abortion rate is high. And because its seeds have harsh requirements on germination conditions, only 1% of the seeds can mature and reproduce smoothly. The reproductive characteristics of Sihemu determine that its reproduction and renewal speed is very slow. In addition, in recent years,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 许亦农平文丽吴韩英李敏春
Owner INST OF BOTANY CHINESE ACAD OF SCI
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