Biomarker for the estimation of acute renal disorder and prognosis of the disorder, and use of the biomarker

A technology of acute kidney injury and biomarkers, applied in the field of new biomarkers, can solve problems such as not getting good results

Inactive Publication Date: 2011-01-26
NAGOYA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, clinical trials of new therapeutic agents such as anti-apoptotic / anti-necrotic agents, anti-inflammatory agents, antiseptic agents, various growth factors, and vasodilators have been conducted for the treatment of AKI, but no good results have been obtained

Method used

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  • Biomarker for the estimation of acute renal disorder and prognosis of the disorder, and use of the biomarker
  • Biomarker for the estimation of acute renal disorder and prognosis of the disorder, and use of the biomarker
  • Biomarker for the estimation of acute renal disorder and prognosis of the disorder, and use of the biomarker

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0116] When the measured value (MK amount) is higher than the reference value, it is judged as "high possibility of onset", and when the measured value is lower than the reference value, it is judged as "low possibility of onset".

example 2

[0118] When reactivity (positive) was confirmed, it was judged as "high possibility of onset", and when reactivity was not confirmed (negative), it was judged as "low possibility of onset".

[0119] (Example of Quantitative Judgment)

[0120] The onset probability (%) was previously set for each range of the measured value as follows, and the onset probability (%) was determined from the measured value.

[0121] Measured values ​​a to b: less than 10% probability of onset

[0122] Measured value b~c: the probability of disease is 10%~30%

[0123] Measured value c~d: the possibility of disease is 30%~50%

[0124] Measured value d~e: the probability of disease is 50%~70%

[0125] Measured value e~f: the possibility of disease is 70%~90%

[0126] In addition to MK, other biomarkers (composed of more than one biomarker selected from urinary NGAL, urinary IL-18, urinary KIM-1, urinary NAG, and urinary L-FABP) are given below. As an example of the judgment method when one or mo...

Embodiment

[0188] 1. Concentration of urinary midkine (MK) in various renal diseases

[0189] (1) object

[0190] MK is a heparin-binding growth factor, and its expression is enhanced in proximal renal tubules during ischemic injury (Sato W. et al., J Immunol. 2001 Sep 15; 167(6): 3463-9.). The correlation between urinary MK concentration and AKI was studied in 583 cases. The details of the 583 cases were: 33 patients with acute tubular necrosis (ATN) which is the main cause of AKI, 517 patients with renal injury other than ATN, and 33 normal volunteers.

[0191] (2) Method

[0192] After obtaining written consent, the urine of each subject was collected to determine the concentration of MK in the urine. Specifically, using 0.01 ml of a urine sample, the concentration of MK in urine was measured by sandwich ELISA using two types of polyclonal anti-MK antibodies (chicken antibody and rabbit antibody).

[0193] (3) Results

[0194] The measurement result (measured value) of MK concent...

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Abstract

Disclosed is a novel biomarker which is useful for the prediction of early onset of acute renal disorder, the estimation of prognosis associated with a renal function, and the diagnosis of acute renal disorder. Also disclosed is use of the novel biomarker. A midkine is used as the biomarker. The determination of the possibility of the onset of acute renal disorder, the estimation of prognosis associated with a renal function or the diagnosis of acute renal disorder can be achieved based on the results of the detection of a midkine in urine.

Description

technical field [0001] The present invention relates to novel biomarkers useful for examination of acute kidney injury, prediction of prognosis related to renal function, and identification of acute kidney injury, and uses of the marker. Background technique [0002] Acute kidney injury (Acute kidney injury, hereinafter also referred to as "AKI") is diagnosed with urine output, serum creatinine (Cr) value, serum urea nitrogen, NAG in urine, β2-microglobulin value, etc., but any one None of the indicators has the diagnostic sensitivity to the degree that can acutely diagnose the pathological state in the acute stage, and the actual situation is to make a diagnosis comprehensively based on the examination results and clinical manifestations. Therefore, the status quo is that too much time is taken until the initiation of effective treatment, and the treatment effect is not good. [0003] Despite the rapid progress in diagnosis and treatment, the mortality rate of patients wit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53
CPCG01N2800/56G01N33/74G01N2333/515G01N2800/347G01N33/53G01N33/68
Inventor 门松健治汤泽由纪夫林宏树松尾清一池松真也
Owner NAGOYA UNIVERSITY
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