Hepatitis B virus affinity adsorption column and preparation method thereof

A hepatitis B virus and adsorption column technology, applied in the direction of selective adsorption, ion exchange regeneration, ion exchange, etc., can solve the problems of easy mutation of hepatitis B virus, inability to achieve therapeutic effect, affecting therapeutic effect, etc., and achieve significant therapeutic effect. , significant curative effect, good blood compatibility

Inactive Publication Date: 2011-02-02
WUHAN ZHENFU PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The current antiviral therapy for the treatment of hepatitis B has high cost, long course of treatment, and large side effects, and cannot achieve a good therapeutic effect. Economic pressure and psychological burden

Method used

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  • Hepatitis B virus affinity adsorption column and preparation method thereof
  • Hepatitis B virus affinity adsorption column and preparation method thereof
  • Hepatitis B virus affinity adsorption column and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0036] Below in conjunction with accompanying drawing and embodiment describe in detail:

[0037] One, a kind of hepatitis B virus affinity adsorption column (abbreviation adsorption column)

[0038] 1. Overall

[0039] Such as figure 1 , the adsorption column includes an affinity microsphere 1, a polyester column 2 and a separation membrane;

[0040] Affinity microspheres 1 are loaded into the polyester column 2, and the two ends of the polyester column 2 are closed by separation membranes 3.

[0041] 2. Function block

[0042] 1) Affinity Microsphere 1

[0043] Using chitosan with a molecular weight of 50,000 to 100,000 to make dark brown spherical particles with a diameter of 150 to 5000 μm to obtain an adsorption microsphere;

[0044] Adding an activator to the adsorption microspheres to obtain activated microspheres;

[0045] Adding hepatitis B human immunoglobulin to the activated microspheres for antibody immobilization to obtain affinity microspheres.

[0046] A...

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PUM

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Abstract

The invention discloses a hepatitis B virus affinity adsorption column and a preparation method thereof, and relates to a blood perfusion technology. The adsorption column comprises affinity microspheres (1), a polyester column (2) and separation membranes (3); and the microspheres (1) are arranged in the polyester column (2), and two ends of the polyester column (2) are closed through the separation membranes (3). The method comprises the following steps: 1) preparing adsorption microspheres; 2) activating the adsorption microspheres and obtaining the activated microspheres; 3) fixing antibodies and obtaining the affinity microspheres; 4) closing unreacted aldehyde and epoxy; 5) filling the column; and 6) sealing with the membranes. The hepatitis B virus affinity adsorption column can assist or replace the anti-virus treatment of hepatitis B, realize significant effects, be applicable to removing hepatitis B virus in blood of hepatitis B virus carriers, chronic hepatitis B patients, patients with severe hepatitis B and the patients with liver cancer and achieve the purposes of relieving, treating and saving lives of the patients. The antibodies against other virus are fixed on the affinity microspheres (1), thereby having good treatment effect for a variety of diseases, such as AIDS and the like.

Description

technical field [0001] The invention relates to a hemoperfusion technology, in particular to a hepatitis B virus affinity adsorption column and a preparation method thereof. Background technique [0002] Blood purification techniques include hemodialysis, hemofiltration, hemodiafiltration, hemoperfusion, plasma separation and therapeutic separation, and blood component adsorption therapy. Since 1979, Terman first reported the use of DNA immunoadsorption column and hemoperfusion technology to treat systemic lupus erythematosus (SLE), which has entered a new stage of immunoadsorption therapy for immune diseases. In recent years, blood purification technology has attracted more and more attention in the in vitro treatment of diseases, and people continue to open up new fields of blood purification technology in the treatment of difficult diseases. [0003] Hepatitis B is a common viral infectious disease that plagues people at present, and it is very popular in my country. Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D15/08A61M1/38
Inventor 王业富李慧
Owner WUHAN ZHENFU PHARMA CO LTD
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