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Surface plasmon resonance sensing element and manufacturing method thereof

A surface plasmon and sensing element technology, applied in the field of sensors for biological system detection, can solve the problems of low sensitivity, easy inactivation of biomolecules, long film forming time, etc., and achieve a wide range of applications

Inactive Publication Date: 2012-02-08
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The sensitive film formed in this way is stable and orderly, but the film formation time is long, the sensitivity is low, and the immobilized biomolecules are easily inactivated

Method used

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  • Surface plasmon resonance sensing element and manufacturing method thereof
  • Surface plasmon resonance sensing element and manufacturing method thereof
  • Surface plasmon resonance sensing element and manufacturing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 The preparation of the surface-formylated magnetic nanoparticles of the present invention.

[0031] Surface aldylated Fe 3 o 4 / SiO 2 The specific preparation process of magnetic nanoparticles is: take 2.6g FeCl 3 ,6H 2 O, 1.0 g FeCl 2 4H 2 O and 0.425mL HCl (12mol L -1 ) was fully dissolved in 15 mL of deionized water, and transferred to the dropping funnel. 125mL concentration 1.5mol L -1 The NaOH solution was added into the three-necked flask, and the dropping funnel was opened under the condition of 80°C, and the mixed solution was added dropwise while fully stirring, and refluxed for 3h. The whole process requires nitrogen protection. After the reaction is completed, the Fe is separated by the action of a magnet. 3 o 4 Particles were thoroughly washed four times with deionized water. Disperse the precipitate into 100 mL of deionized water to obtain Fe 3 o 4 Magnetic Nanoparticle Solution. Take 0.724mL Fe 3 o 4 Add the magnetic nanoparticl...

Embodiment 2

[0037] The characterization of the magnetic nanoparticle of embodiment 2 surface aldylation

[0038] Fe 3 o 4 The magnetic nanoparticles are made of Fe 2+ and Fe 3+ The salt solution is prepared by co-precipitation under alkaline conditions. Its surface morphology can be characterized by transmission electron microscopy. Its spectral properties are detected by infrared spectroscopy and ultraviolet-visible absorption spectroscopy.

[0039] image 3 , Figure 4 , Figure 5 Respectively Fe 3 o 4 Magnetic nanoparticles, Fe 3 o 4 / SiO 2 Magnetic nanoparticles, Fe 3 o 4 / Ag / SiO 2 Electron microscope image of magnetic nanoparticles. Figure 6 is the infrared spectrum of magnetic nanoparticles, (a) Fe 3o 4 Nanoparticles, (b)Fe 3 o 4 / SiO 2 Nanoparticles, (c)Fe 3 o 4 / Ag / SiO 2 Nanoparticles. From Figure 6 It can be seen that Fe 3 o 4 Nanoparticles at 590cm -1 There is an obvious absorption peak at , which is because of the Fe-O structure it contains. whil...

Embodiment 3

[0040] Embodiment 3 Fabrication of the sensing element of the present invention.

[0041] figure 2 is a schematic diagram of the sensing element based on magnetic nanoparticles of the present invention.

[0042] Two novel sensing elements based on magnetic nanoparticle modification assembled in the present invention. The specific implementation method is that the bottom surface of the glass prism coated with the metal film is placed above, then the flow cell is fixed above the glass prism, and the magnet is fixed below the glass prism. Inject PBS buffer solution into the flow cell as a baseline solution. Then inject surface aldehyde-modified Fe respectively 3 o 4 / SiO 2 Magnetic nanoparticles, Fe 3 o 4 / Ag / SiO 2 The magnetic nanoparticle solution was monitored in real time for 5 hours to observe the shift of its resonance wavelength. Due to the action of the magnet, the magnetic nanoparticles are easily fixed on the gold film, which leads to the change of the refracti...

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Abstract

The invention provides a surface plasmon resonance sensing element and a manufacturing method thereof, belonging to the technical field of a sensor for biological system detection. The sensing element comprises a metallic membrane (2), a sensitive membrane and a magnet (3), wherein, the metallic membrane with a chromium membrane and a gold membrane as the sensing elements is plated on the bottom surface of a glass prism (1), the sensitive membrane composed of magnetic nanoparticles (4) subject to surface hydroformylation and an antibody (5) linked with an aldehyde group through a covalent bond is made on the metallic membrane, and the magnet is arranged under the glass prism. The manufacturing method of the sensing element comprises the following steps: preparation of the metallic membrane, preparation of Fe3O4 magnetic nanoparticles, preparation of ammoniated SiO2-coated magnetic nanoparticles, preparation of magnetic nanoparticles subject to surface hydroformylation and preparation of the sensitive membrane. The invention has the advantages of simple manufacturing process, high sensitivity, good biocompatibility and good selectivity and can be used in all antibody and antigen detection and protein and drug measurement.

Description

technical field [0001] The invention belongs to the technical field of sensors used for biological system detection, and in particular relates to the preparation and application of surface plasmon resonance sensors based on magnetic nanoparticle modification. Background technique [0002] Surface plasmon resonance (SPR) is a physical optical phenomenon. Using the evanescent wave when light undergoes total internal reflection at the two-phase interface, the free electrons on the metal surface are induced to generate surface plasmons. At a certain wavelength or incident angle, the frequency of the surface plasmon and the evanescent wave are equal, and the two will resonate, the incident light is absorbed, and the energy of the reflected light drops sharply, so that a resonance peak appears on the reflection spectrum. When the refractive index of the medium on the surface of the metal film is different, the position of the resonance peak will be different. Due to the direct, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/55G01N33/553G01N33/577C01G49/06C01B33/12G01N21/552
Inventor 宋大千孙颖王丽英王键汪静曹彦波
Owner JILIN UNIV
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