Method for comprehensively using pig blood

A pig blood and plasma technology, applied in the field of separation and extraction of biologically active substances, can solve the problems of single product production, long time consumption, high energy consumption of the process, etc., achieve easy absorption and utilization, reduce production costs, and overcome the effect of single product

Inactive Publication Date: 2011-02-23
江苏省江大绿康生物工程技术研究有限公司
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AI Technical Summary

Problems solved by technology

From the direct consumption of pig blood tofu to the production of enzymatic pig blood meal and compound amino acid powder, great progress has been made, but it still cannot meet the needs of human beings.
The problem is that most of the processes are not perfect. For example, some processes consume a lot of energy and take a long time, some processes can only produce a single product, some processes have low yield, some processes use a large amount of organic solvents, and there are environmental protection defects, etc.

Method used

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  • Method for comprehensively using pig blood
  • Method for comprehensively using pig blood
  • Method for comprehensively using pig blood

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0017] Add 500 g of trisodium citrate dissolved in a small amount of deionized water to 50 L of quarantine-qualified fresh pig blood, stir well, and let stand overnight below 4°C. According to step 2 of the above-mentioned invention method, about 35 L of plasma and about 15 L of blood cells were obtained. The blood plasma is operated according to step 3 of the above-mentioned inventive method, diluted with 8 times normal saline and then adjusted to pH 5.2 with 2 mol / L acetic acid, left standing at low temperature overnight, and the precipitated prothrombin is collected. Precipitation of prothrombin was performed according to steps 4 and 5 of the above-mentioned inventive method without freeze-drying to obtain 1.8 L of refined thrombin solution.

[0018] Add an appropriate amount of normal saline to 20L blood cells frozen overnight to make the substrate concentration 8-10%, heat up to about 10°C, stir at high speed for 20 minutes, operate according to steps 7 and 8 of the above...

example 2

[0020] Thrombin activity detection, porcine blood hydrolyzate protein component analysis test:

[0021] 1) Materials: standard thrombin, fibrinogen, and bovine serum albumin (purchased from China Institute for the Control of Pharmaceutical and Biological Products); thrombin samples (made by the method in Example 1).

[0022] 2) Detection method:

[0023] ①Thrombin activity is detected according to the method shown in the Pharmacopoeia of the People's Republic of China (Part Two) (pages 1056-1057 of the 2000 edition);

[0024] ② Determination of protein content by Folin - phenol method.

[0025] ③ Hydrolyzed amino acid: high performance liquid chromatography.

[0026] 3) Results: Take 3 batches of 100ml each of the samples prepared by the above-mentioned inventive method continuously, and see Table 1 and Table 2 for the test results according to the above-mentioned detection method.

[0027] Table 1 sample detection data

[0028]

[0029] Note: The total activity refers ...

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Abstract

The invention belongs to the technical field of separation and extraction of bioactive substance and particularly relates to a method for preparing thrombin and pig blood protein hydrolysate respectively by taking blood plasma and blood corpuscles as a starting substrate after the anticoagulant treatment of fresh pig blood. The high-activity thrombin is prepared by the following steps of: freezing the blood plasma at low temperature; precipitating and collecting prothrombin; activating ions; purifying enzyme liquid and the like; and the high-activity pig blood protein hydrolysate is prepared by the following steps of: breaking the walls of the blood corpuscles; dissolving blood, performing segmental enzymolysis on complex enzyme; filtering and degerming and the like. The amino acid content of the pig blood protein hydrolysate prepared by the method accounts for over 80 percent of the protein, and the pig blood protein hydrolysate is easy to absorb and use. In the method, raw materialsare fully used and two high value-added products can be produced by feeding once. The whole production process saves high-toxicity organic solvents so as to overcome the defects of single product, solvent residues and the like and reduce production cost. By using the method, over 6,000U of thrombin competitive products and over 150g of high-activity pig blood protein hydrolysate powder can be prepared from1 L of pig blood.

Description

technical field [0001] The invention relates to a method for simultaneously extracting thrombin and pig blood hydrolyzed protein from pig blood, belonging to the technical field of separation and extraction of biologically active substances. Background technique [0002] Pig blood is a high-quality protein, and nutritionists call it liquid meat. Pig blood contains almost all the nutrients needed by the human body: protein, amino acids, vitamins, sugar and sodium, potassium, iron, calcium and other indispensable trace elements for the human body. Among them, 18.9% protein, 1.2% non-protein organisms, 0.9% minerals, and 79% water. In addition, its amino acid composition is balanced, and the total amount of 8 essential amino acids required by the human body is higher than that of human milk and whole eggs, especially lysine The amino acid content is as high as 9%, which is in line with the recommended mode of the United Nations Food and Agriculture Organization and the World H...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C12N9/74
Inventor 吴保承杨春霞
Owner 江苏省江大绿康生物工程技术研究有限公司
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