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Active constituent extracted from plant of altingia yunnanensis and application thereof

An active ingredient and plant technology, applied in the directions of plant/algae/fungus/moss ingredients, medical preparations containing active ingredients, plant raw materials, etc., can solve the problem of no anti-Bacillus cereus activity, etc., and achieve a strong inhibitory effect Effect

Inactive Publication Date: 2011-04-06
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main use of Mengzi mushroom tree (A.yunnanensis) is as a local wood species, and there is no public report about the plant's anti-Bacillus cereus activity

Method used

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  • Active constituent extracted from plant of altingia yunnanensis and application thereof
  • Active constituent extracted from plant of altingia yunnanensis and application thereof
  • Active constituent extracted from plant of altingia yunnanensis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The branches of the Mengzi mushroom tree were washed with water, put into an oven, and baked at 60°C for 12 hours. 10 grams of dried branches were chopped, put into a conical flask, soaked in 100ml of 100% methanol, and extracted on a water bath at 40°C. The time for soaking and extracting for the first time is 2 hours, and the time for soaking and extracting for the second time and the second time is 4 hours and 8 hours respectively. The extracts from the three extractions were combined and concentrated at 60°C to obtain the extract. The extract was dissolved in methanol and prepared to a concentration of 10 mg / ml for activity determination. At the same time as a comparison, the results are shown in Table 1.

[0021] Table 1 The antibacterial effect of the extract of Mushroom branch on Bacillus cereus

[0022]

[0023] - No zone of inhibition appears

[0024] The result shows: the active ingredient that the present invention extracts has obvious antibacterial ac...

Embodiment 2

[0026] Basically the same as embodiment one, the difference is that the material used is the leaves of the Mengzi mushroom tree. The results are shown in Table 2.

[0027] Table 2 The antibacterial effect of the extract of Mushroom leaves on Bacillus cereus

[0028]

[0029] - No zone of inhibition appears

[0030] Compared with branch extracts, leaf extracts have relatively stronger antibacterial activity. When the extract is at 1mg, the size of the bacteriostatic zone is 1.4cm, and when 0.1mg, the antibacterial activity also occurs, the activity is relatively weak, and the size of the bacteriostatic zone is 0.9cm.

Embodiment 3

[0032] Basically the same as Example 2, the difference is that the extracted extract is heated in boiling water at 100°C for 30 minutes, then dissolved in methanol, and prepared at a concentration of 10 mg / ml for activity. At the same time as a comparison, the results are shown in Table 3.

[0033] Table 3 The antibacterial effect of the extract of Mushroom leaves on Bacillus cereus after high temperature treatment

[0034]

[0035] - No zone of inhibition appears

[0036] The results showed that the samples after high temperature treatment had the same antibacterial activity as the samples without high temperature treatment, indicating that the active ingredient was stable to heat.

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PUM

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Abstract

The invention relates to an active constituent extracted from a plant of altingia yunnanensis and application thereof, belonging to the technical field of biological medicine. The active constituent extracted from the plant of the altingia yunnanensis is obtained through the following steps of: a. washing and cleaning branches or leaves of the altingia yunnanensis with water and baking the cleaned branches or leaves in a baking oven for 12 hours at 60 DEG C; b. cutting the baked branches or leaves of the altingia yunnanensis into pieces, putting in a triangular flask, soaking the cut branches or leaves with 100 percent methanol and extracting the cut branches or leaves on a water bath kettle at 40 DEG C, wherein the soaking extraction of the first time lasts for 2 hours, and the soaking extraction of the second time lasts for 4 hours and the soaking extraction of the third time lasts for 8 hours; c. merging extracting liquid obtained by extracting three times and concentrating the extracting liquid at 60 DEG C to obtain extractum; and d. dissolving the extractum with methanol, metering the volume to be 10mg / ml and placing in a refrigerator at 4 DEG C, and storing for later use. The extracted active constituent has stronger inhibiting effect on human pathogenic bacteria of bacillus cereus, can be used for preparing preparations for inhibiting the human pathogenic bacteria of the bacillus cereus and can be developed into novel medical antibiotics.

Description

Technical field: [0001] The invention relates to an active ingredient extracted from the plant Mengzi mushroom and its application, belonging to the technical field of biomedicine. Background technique: [0002] Bacillus cereus belongs to the Bacillus family, the genus Bacillus. This bacterium is widely distributed in nature. Studies have shown that this bacterium can produce a variety of toxic substances, such as enterotoxins, hemolytic toxins, etc. The food poisoning caused by it involves many types of food, and the potential risk of food poisoning is high. Human diseases caused by ubiquitous bacteria are of concern. The antibiotics discovered so far are mainly derived from plants and microorganisms, many of which have been widely used to treat various microbial infectious diseases. In recent years, due to the drug resistance of pathogenic bacteria, the original medical antibiotics are declining, so the continuous development of new antibiotics has been paid attention to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P31/04A61K127/00A61K135/00
Inventor 张克勤李国红周薇
Owner YUNNAN UNIV
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