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Application of microRNA-139

1. Microrna-139, a transferable technology, applied in the field of biomedical materials, can solve the problems of restricting promotion and application, producing drug resistance, etc., and achieve the effect of overcoming drug resistance, releasing inhibition, and inhibiting migration and invasion

Inactive Publication Date: 2011-04-06
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Description
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Problems solved by technology

In clinical studies, it was found that some patients receiving Herceptin treatment developed obvious cardiotoxicity, and most of the patients who received effective treatment developed drug resistance within one year, which limited its further promotion and application

Method used

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  • Application of microRNA-139
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  • Application of microRNA-139

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Embodiment Construction

[0044] 1. The expression of CXCR4 is upregulated after miR-139 is inhibited by HER2 in tumor cells.

[0045]The epidermal growth factor family receptor molecule HER2 expressed on the surface of tumor cells is closely related to tumor metastasis, while the chemokine receptor CXCR4 is recognized as an important molecule that mediates tumor cell migration and invasion. Whether HER2 signaling upregulates CXCR4 expression through mechanisms including miRNAs is unclear. We used the metastatic HER2-positive gastric cancer SGC-7901 cell line as the research object. After inhibiting the expression of HER2, we applied miRNA chip technology to screen and obtain multiple miRNAs with up-regulated expression. In addition to the miR-146a that has been reported to inhibit the expression of CXCR4, we used miRNA Viewer bioinformatics software to predict that human miR-139 (Gene ID: 406931; MI0000261, microrna.sanger.ac.uk) may also be a regulator of CXCR4 miRNA (such as figure 1 shown in a)....

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Abstract

The invention discloses an application of microRNA-139 (miR-139 for short) in preparing a therapeutic drug and diagnostic reagent for metastatic tutors. The miR-139 acts on the chemokine receptor CXCR4 of a target gene to restrain the metastasis and intrusion of tumor cells. In the tumor cells, a human epidermal growth factor family molecule HER2 interacts with CD44 so as to regulate down the expression of the miR-139, therefore, by means of detecting the expressions of the miR-139 as well as the correlative HER2, CD44 and CXCR4, early diagnosis can be carried out on the tumors, and the metastasis and prognosis of the tumors can be evaluated; and by means of expressing the miR-139 in HER2 positive tumors or interfering the upstream signal of the miR-139 to recover the expression of endogenous miR-139, the metastasis of the tumors can be restrained.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and relates to the use of a small molecule RNA-microRNA-139 (hereinafter referred to as miR-139) in the preparation of therapeutic drugs and diagnostic reagents for metastatic tumors. Background technique [0002] 1. The role of microRNA (miRNA) in the malignant phenotype of tumor cells and its expression regulation mechanism [0003] miRNA, as a non-coding small nucleic acid that researchers have gradually paid attention to in recent years, inhibits the translation of specific target molecules by binding to the 3'UTR of protein-coding gene transcription products, thereby inhibiting gene expression at the post-transcriptional level, and then affecting its downstream Regulatory molecules carry out normal biological functions. Tumor-associated miRNAs can be roughly divided into two categories, tumor miRNAs (Oncomir) and tumor suppressor-associated miRNAs, which are defined mainly by t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61K31/7105G01N33/574
Inventor 杨安钢贾林涛鲍炜付海京谢桥生张瑞王磊孟艳玲
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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