Method for preparing solid lipid nanoparticles of water-soluble anti-tumor medicine

A solid lipid nanotechnology and anti-tumor drug technology, which is applied in the field of pharmacy, can solve the problems of unstable encapsulation rate, achieve the effect of improving encapsulation rate, reducing drug burst release, and low requirements for preparation materials

Inactive Publication Date: 2011-05-11
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this preparation method uses unconventional materials: n-decyl salicylic phosphate or hexadecyl phosphate, and its encapsulation efficiency depends on the combination of covalent bonds, and the encapsulation efficiency is unstable.

Method used

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  • Method for preparing solid lipid nanoparticles of water-soluble anti-tumor medicine
  • Method for preparing solid lipid nanoparticles of water-soluble anti-tumor medicine
  • Method for preparing solid lipid nanoparticles of water-soluble anti-tumor medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The preparation method of the present invention is as follows: 0.2 g of stearic acid is weighed, dissolved in 6 mL of acetone-ethanol mixed solution with a volume ratio of acetone and ethanol of 1:2, and then mixed with 2 mL of lecithin ethanol solution, wherein the lecithin ethanol solution is composed of 100 mg Lecithin was prepared by dissolving 2 mL of ethanol, and 1 mL of anti-tumor drug solution was added dropwise with stirring in a water bath at 73 °C. The anti-tumor drug solution was prepared by dissolving 3 mg of doxorubicin and 13 mg of pluronic F87 in 1 mL of water, and continued stirring for 10 min. , slowly inject 400 μL of 2.5% sodium alginate, quickly adjust the temperature of the water bath to 68 °C, continue stirring for 15 minutes, ultrasonicate the probe in the water bath, work for 2 s, intermittently for 3 s, and sonicate at 400 W for 50 times, then quickly pour into 40 mL of 4 °C pre-cooled water , continue to stir for 30 minutes, disperse to form so...

Embodiment 2

[0026] The pluronic F87 was changed to pluronic F88, and the others were the same as in Example 1, to obtain the pluronic F88 doxorubicin solid lipid nanoparticles with an encapsulation efficiency of 81.9±1.8% and a particle size of 357±90 nm.

Embodiment 3

[0028] The pluronic F87 was changed to pluronic P85, and the others were the same as in Example 1, to obtain pluronic P85 doxorubicin solid lipid nanoparticles with an encapsulation efficiency of 78.6±1.7% and a particle size of 365±78 nm.

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Abstract

The invention belongs to the field of pharmaceutics and particularly relates to a method for preparing solid lipid nanoparticles of water-soluble anti-tumor medicine. The method comprises the steps of: dissolving stearic acid and lecithin in organic solvent, mixing, dropping anti-tumor medicine under the condition of water bath, stirring and slowly injecting polyanion sodium alginate so as to packing the medicine and the sodium alginate in nanoparticles, and acquiring the solid lipid nanoparticles by temperature changing, probe ultrasonic treatment and filter membrane treatment. In the invention, the adriamycin capsulated in the solid lipid nanoparticles is combined with the alginate so as to greatly increase the encapsulation rate, lower the absorption of surface medicine, and delay the release of the anti-tumor medicine for achieving the purpose of reducing the suddenly releasing of the medicine. Compared with the other method for increasing the encapsulation rate, the method provided by the invention is simple to operate, is low in preparing material demand and high in encapsulation rate, and can efficiently overcome multidrug resistance.

Description

technical field [0001] The invention belongs to the field of pharmacy, in particular to a preparation method of a water-soluble antitumor drug solid lipid nanoparticle. Background technique [0002] Solid lipid nanoparticles are a new type of drug delivery carrier that has developed rapidly in recent years. The particle size of solid lipid nanoparticles is about 50-1000 nm, which is solid at room temperature and has the following characteristics: (1) good biocompatibility; (2) can effectively control the release of drugs, and can effectively avoid the degradation of drugs and leakage; (3) suitable for a variety of administration routes; (4) good stability, can improve the stability of unstable drugs. Solid lipid nanoparticles have these advantages and are considered to be a promising new drug delivery system carrier. In addition, solid lipid nanoparticles have also shown special advantages in many diseases, especially in cancer treatment. [0003] At present, for the prep...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/14A61K45/00A61K31/704A61K47/36A61J3/00A61P35/00
Inventor 许东航
Owner ZHEJIANG UNIV
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