Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for culturing directly differentiated tissues of cotton true leaf and special culture medium

A technology of culture medium and germination medium, which is applied in the field of plant tissue culture, can solve the problems of low regeneration frequency, lack of difficulty, poor leaf and stem segments, etc., and achieve the effect of easy material selection and shortening the culture period

Inactive Publication Date: 2012-11-21
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The hypocotyl is the easiest, followed by the mesocotyl and epicotyl, the cotyledons are poor, and the leaves and stems are the worst. In recent years, hairy roots can be regenerated, but there is no comparison of the degree of difficulty (Jiao Gaili et al., 2002); Zhang Hai et al. (2002) reported the in vitro culture and plant regeneration of cotton cotyledons, but the regeneration frequency was not high.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for culturing directly differentiated tissues of cotton true leaf and special culture medium
  • Method for culturing directly differentiated tissues of cotton true leaf and special culture medium
  • Method for culturing directly differentiated tissues of cotton true leaf and special culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1, acquisition of cotton regenerated seedlings and statistical observation thereof

[0032] 1. Preparation of embryogenic callus

[0033] 1) Pretreatment of explants

[0034] The true leaves of field cotton plants were taken as explants. After taking back the cotton leaves, prepare 8 high-temperature sterilized (121°C, 14min) petri dishes, add appropriate amount of sterilized water (121°C, 14min) to No. 1, 6, 7, and 8 petri dishes, and 2, 3, 4 add HgCl with a mass percentage concentration of 0.1% in the petri dish 2 solution.

[0035] Among them, there are 3 varieties of cotton, which are:

[0036] China Cotton Research Institute 24, sold by the Technology Trading Company of the Cotton Research Institute of the Chinese Academy of Agricultural Sciences;

[0037] Jihe 321, sold by the Technology Trading Company of the Cotton Research Institute of the Chinese Academy of Agricultural Sciences;

[0038]China Cotton Research Institute 27, sold by the Technolo...

Embodiment 2

[0058] Embodiment 2, the acquisition of cotton regenerated seedlings and statistical observation thereof

[0059] The difference between this embodiment and embodiment 1 lies in the following three points, and all the other steps are identical:

[0060] 1. The raw material used is only China Cotton Research Institute 24, which is cultivated by the early-maturing breeding research group of the Cotton Research Institute of the Chinese Academy of Agricultural Sciences and sold by the Science and Technology Trading Company of the Cotton Research Institute of the Chinese Academy of Agricultural Sciences.

[0061] 2. EC1 medium: Add kinetin (KT), 6-BA, 2,4-D, IAA, glucose, Gel rite on the basis of MSB1 basic medium; the final concentration of KT is 0.005mg / L , The final concentration of 6-BA is 0.005mg / L, the final concentration of 2,4-D is 0.0001mg / L, the final concentration of IAA is 0.001mg / L, the final concentration of glucose is 25g / L, the final concentration of Gel rite The c...

Embodiment 3

[0066] Embodiment 3, the acquisition of cotton regenerated seedlings and statistical observation thereof

[0067] The difference between this embodiment and embodiment 1 lies in the following three points, and all the other steps are identical:

[0068] 1. The raw material used is only China Cotton Research Institute 24, which is cultivated by the early-maturing breeding research group of the Cotton Research Institute of the Chinese Academy of Agricultural Sciences and sold by the Science and Technology Trading Company of the Cotton Research Institute of the Chinese Academy of Agricultural Sciences. ;

[0069] 2. EC1 medium: Add kinetin (KT), 6-BA, 2,4-D, IAA, glucose, Gel rite on the basis of MSB1 basic culture medium; among them, the final concentration of KT is 0.15mg / L , The final concentration of 6-BA is 0.15mg / L, the final concentration of 2,4-D is 0.001mg / L, the final concentration of IAA is 0.02mg / L, the final concentration of glucose is 35g / L, the final concentration...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for culturing directly differentiated tissues of a cotton true leaf and a special culture medium. The special culture medium provided by the invention is a culture medium which is used when embryogenic callus is directly differentiated from a cotton leaf; and the culture medium is obtained by adding KT, 6-BA, 2,4-D, indole acetic acid (IAA), glucose and gel into marek's disease 1 (MSB1) basic culture solution, wherein the final concentration of the KT, the 6-BA, the 2,4-D, the IAA, the glucose and the Gel rite is 0.005 to 0.15mg / L, 0.005 to 0.15mg / L, 0.0001 to0.001mg / L, 0.001 to 0.02mg / L, 25 to 35g / L and 1.8 to 2.5g / L respectively. By the method, a tissue culture system is established by taking leaves of field cotton plants as explants; and finally, a stable and high-efficiency tissue culture system of the directly induced embryogenic callus of the leaf is established.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a special culture medium for a field cotton plant true leaf tissue culture method. Background technique [0002] In cotton tissue culture, somatic embryogenesis and plant regeneration can be divided into four steps: explant dedifferentiation induces callus, callus redifferentiates into embryogenic callus, embryogenic callus cultures somatic cells Embryos and somatic embryos germinated to obtain plant regeneration. It is relatively easy to grow callus from explants by dedifferentiation, and there is no report of genotype restriction. The induction and differentiation of embryogenic callus from callus is the bottleneck of cotton tissue culture, and the reports limited by genotype focus on the induction stage of embryogenic callus. [0003] Genotype restriction is firstly manifested in the different somatic embryogenesis and plant regeneration abilities of different cotton spec...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 张朝军李付广王晔王玉芬武芝侠李凤莲
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com