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High-efficient induction method of tetraploid cynoglossus semilaevis fish fries

A semi-smooth tongue sole and tetraploid technology, which is applied to the field of efficient induction of tetraploid semi-smooth tongue sole fry, biotechnology methods to induce semi-smooth tongue sole tetraploid, can solve problems such as no breakthroughs, and achieve neat ploidy, simple operation, and high induction efficiency Effect

Inactive Publication Date: 2011-06-15
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

However, so far, there have been no reports at home and abroad on the mass induction of tetraploid fry of semi-smooth tongue sole, and the mass production of triploid sterile fry through tetraploid and diploid mating.
In particular, there have been no breakthroughs in key technologies such as how long to carry out hydrostatic pressure treatment after fertilization, how much pressure to use, and how long to process to obtain tetraploid fry.

Method used

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  • High-efficient induction method of tetraploid cynoglossus semilaevis fish fries

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Embodiment Construction

[0026] The invention adopts the means of modern biotechnology, establishes the tetraploid induction technology of semi-smooth tongue sole, and develops the tetraploid fry.

[0027] Semi-smooth tongue sole eggs were fertilized with homologous sperm, and the fertilized eggs were subjected to hydrostatic shock treatment for a certain period of time after insemination to inhibit the first cleavage and double the chromosomes, which can successfully induce the production of tetraploid fry of tongue-sole semi-smooth. The establishment of high-efficiency induction technology for tetraploid fry of tongue sole has important practical significance and great significance for sex control of tongue sole, production of sterile triploid seedlings, breeding of sterile seedlings, and improvement of breeding yield, product quality and economic benefits of tongue sole. promotion potential.

[0028] The technical content of the present invention is described in detail below:

[0029] The technica...

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Abstract

The invention relates to a high-efficient induction method of tetraploid cynoglossus semilaevis fish fries, and the method comprises the steps of induction of the tetraploid cynoglossus semilaevis fish fries and identification of the tetraploid cynoglossus semilaevis fish fries, and is characterized by performing pressure shock treatment for 3.5-6 minutes under hydrostatic pressure of 38-44MPa within 19-28 minutes after fertilization; and transferring fertilized eggs into seawater at the temperature of 19-23 DEG C for culture and incubation after completing the shock treatment. According to the invention, a technical method which can be used for effectively inducing the fertilized eggs of cynoglossus semilaevis to produce tetraploid fish fries in a doubled manner is established, and a technical parameter for inhibiting the first cleavage of the eggs of the cynoglossus semilaevis is firstly determined. The method is simple to operate, strong in practicality, easy to implement, safe and reliable; and the induction efficiency of the tetraploid fish fries is high. By adopting the method, a new technical approach is provided for the sex control and polyploid breeding of the cynoglossus semilaevis.

Description

technical field [0001] The invention belongs to the technical field of aquatic genetics and breeding, and in particular relates to a method for efficiently inducing tetraploid tongue sole fry, that is, adopting a biotechnology method to induce the tetraploid of tongue sole. Background technique [0002] Semi-smooth tongue sole (Cynoglossus semiliaevis) belongs to Pleuronectiformes, family Cynoglossidae, and genus Cynoglossus. Because of its delicious taste, tender meat and rich nutrition, it is very popular among consumers, and its market value is extremely high. Half-smooth tongue sole farming has been widely carried out in the coastal areas of my country, and its annual output value of the farming industry reaches 1.5-2 billion yuan. However, due to the large size difference between the male and female half-smooth tongue sole, the slow growth rate of male individuals reduces the aquaculture output, and the large-scale female fish has excessively developed gonads and obvio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 陈松林季相山李文龙谢明树徐营
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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