Method for obtaining refined extract from capparis spinosa and application of extract

A technology of capers and extracts, which is applied in the field of natural medicinal chemistry, can solve the problems that are not conducive to industrial production, the chemical composition of the active part is not clear, and the process is cumbersome, so as to achieve the effect of being beneficial to ecological balance, clear chemical composition and simple process

Inactive Publication Date: 2011-06-15
SHANGHAI INST OF PHARMA IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The process of this method is cumbersome, not conducive to industrial production, and the material basis such as the chemical composition of the active site is not clear

Method used

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  • Method for obtaining refined extract from capparis spinosa and application of extract
  • Method for obtaining refined extract from capparis spinosa and application of extract
  • Method for obtaining refined extract from capparis spinosa and application of extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Capers dried fruit 1kg, pulverized, extracted with 8 times the volume of water, extracted twice, each time for 2 hours, filtered, and combined the two filtrates. Concentrate under reduced pressure to a concentration of about 1.2 g / ml. 95% ethanol was added to make the final alcohol concentration 70%, and the alcohol precipitation was allowed to stand overnight. Filtrate to obtain the filtrate, concentrated under reduced pressure to no alcohol smell, this solution was passed through 860021 type macroporous adsorption resin, followed by water, 30%, 50%, 70%, 95% ethanol (both volume ratio) elution, to obtain water Part 131.5g, 30% ethanol part 14.70g, 50% ethanol part 3.328g, 70% ethanol part 1.304g, 95% ethanol part 0.66g.

Embodiment 2

[0084] Capers dried fruit 1kg, pulverized, extracted with 8 times the volume of water, extracted twice, each time for 2 hours, filtered, and combined the two filtrates. Concentrate under reduced pressure to a concentration of about 1.2 g / ml. 95% ethanol was added to make the final alcohol concentration 70%, and the alcohol precipitation was allowed to stand overnight. Filtrate was obtained by filtration, concentrated under reduced pressure to no alcohol smell, passed this solution through 860021 macroporous adsorption resin, and eluted with water, 30%, 70%, and 95% ethanol (both by volume ratio) successively to obtain 129.8 g of the water part , 30% ethanol part 13.6g, 70% ethanol part 4.2g, 95% ethanol part 0.59g.

Embodiment 3

[0086] Capers dried fruit 1kg, crushed, extracted with 8 times the volume of 75% ethanol, heated and refluxed twice, each time refluxed for 2 hours, heated and refluxed twice, each time refluxed 2 hours, filtered, combined twice filtrate. Concentrate until there is no alcohol smell, add an appropriate amount of ethanol solution, and dilute to a certain volume of 30% ethanol solution. This solution was passed through 860021 type macroporous adsorption resin, followed by gradient elution with water, 30%, 70%, 95% ethanol (both volume ratios), to obtain 78.8g of the water part, 12.2g of the 30% ethanol part, and 70% ethanol Part 8.54g, 95% ethanol part 3.4g.

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Abstract

The invention discloses a method for obtaining a refined extract from capparis spinosa. The method for obtaining the refined extract from the capparis spinosa is characterized by comprising the following steps of: a) performing macroporous adsorption resin chromatography on a concentrated capparis spinosa crude extract and performing elution by using water and 20 to 30 percent ethanol solution sequentially; and b) performing elution by using 50 to 100 percent ethanol solution to obtain the capparis spinosa refined extract. The invention also provides the capparis spinosa refined extract prepared by the method. A medicament effect test proves that the capparis spinosa refined extract has obvious anti-rheumatoid arthritis activity. The method is simple in process and suitable for industrialized production.

Description

technical field [0001] The invention relates to the field of natural medicinal chemistry, in particular to a method for obtaining a refined extract from capers and the application of the extract. Background technique [0002] Capers are Capparis spinoa L, a plant of the cauliflower family, also known as mallet fruit vine, mouse melon, wild watermelon, pineapple fruit, etc. Capers are a kind of semi-shrubs that grow in hot and arid climate conditions. In my country, they are mainly distributed in low rocky mountains, hilly slopes, gullies at the foot of mountains or Gobi Desert in the north and south of Tianshan Mountains in Xinjiang. In addition, it also grows in Gansu, Tibet and other places. Capers are mainly distributed in the Mediterranean Gulf region abroad, and the growth period is from May to October. The medicinal parts of capers are leaves, fruit and root bark. It tastes pungent and warm in nature. Folks use capers to treat arthritis, ventilation and other disease...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P19/02A61P19/04A61P29/00
Inventor 吴彤李燕刘全海周海凤黄晓玲檀琼于美静张乐乐刘珉宇杨方姜仁吉闫兆峰
Owner SHANGHAI INST OF PHARMA IND
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