Prophase culture solution and anaphase culture solution for porcine early embryonic development

An embryo development and culture medium technology, applied in the field of developmental biology, can solve the problems of imperfect embryos, lack of paracrine factors in the female reproductive tract, and the quantity and quality of embryos are not as good as in vivo embryos.

Inactive Publication Date: 2011-06-15
SHANGHAI ACAD OF AGRI SCI
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Problems solved by technology

In addition, the in vitro culture system also lacks any female reproductive tract paracrine factors
Generally speaking, the number and quality of embryos cultured in vitro at this stage are inferior to those i

Method used

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  • Prophase culture solution and anaphase culture solution for porcine early embryonic development
  • Prophase culture solution and anaphase culture solution for porcine early embryonic development
  • Prophase culture solution and anaphase culture solution for porcine early embryonic development

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Embodiment 1

[0019] Embodiment 1 Pig early embryo pre-culture fluid and late-stage culture fluid of the present invention

[0020] Table 1 NCSU23, NCSU37 and the composition of the pre- and late-stage culture fluid of pig early embryo development of the present invention

[0021] Note: The above liquid is prepared according to the volume of 100mL, and the unit is grams. (The unit of the numbers outside the brackets is grams, and the numbers in the brackets are the converted concentrations in mmol / L)

[0022]

[0023] The culture solution in Table 1 was prepared according to conventional methods well known to those skilled in the art using embryo testing grade ultrapure water as a solvent. Wherein, the pig serum on the 7th day of estrus is prepared according to the following method: select sows in good body condition, healthy body and normal estrus cycle from multiparous breeding sows for blood collection. With several 0 days of the first mating, 10 mL of aseptic blood was collected f...

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Abstract

The invention discloses prophase culture solution (foregoing 60h) for porcine early embryonic (applicable to embryos before porcine blastocysts are hatched) development and anaphase culture solution (from 60h after the culture to a blastocyst hatching stage) for porcine early embryonic development. Calcium lactate, sodium pyruvate, beta-mercaptoethanol, dbcAMP.Na, nonessential amino acid and oestrus 7dth porcine serum are added on the basis of NCSU23 culture solution without CaC12. In addition, the difference between the prophase culture solution for porcine early embryonic development and the anaphase culture solution for porcine early embryonic development is that: the prophase culture solution for porcine early embryonic development does not contain glucose, and the anaphase culture solution for porcine early embryonic development contains the glucose; and particularly, when porcine early embryos are cultured, the prophase culture solution for porcine early embryonic development is used for culturing first, and the anaphase culture solution for porcine early embryonic development is used after 60h. Therefore, the developmental rate of the blastocysts and the quality of the blastocysts can be greatly improved.

Description

technical field [0001] The invention relates to developmental biology, in particular to a culture solution for the early stage of pig early embryo development and a culture solution for the later stage of pig embryo development. Background technique [0002] Early embryo culture is the basis and key link of embryo biotechnology, which is of great significance for in vitro fertilization, embryo segmentation, embryo chimerism, nuclear transfer and the construction of transgenic animals. The existing early embryo culture system can successfully support the development of reconstituted embryos to the blastocyst stage, but the rate of development to blastocysts is not as high as in vivo, and the quality of embryos cultured in in vitro culture systems is also worse than that of in vivo embryos. People still know little about the mechanism of early embryo development, and in vitro culture can not completely simulate the internal environment: on the one hand, the embryos in vivo are...

Claims

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Application Information

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IPC IPC(8): C12N5/073
Inventor 张德福戴建军吴彩凤张树山张廷宇
Owner SHANGHAI ACAD OF AGRI SCI
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