Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for identifying bifidobactirium

A technology of bifidobacteria and analysis method, which is applied in the field of molecular biology to identify bifidobacteria, and can solve the problems of distinguishing species, difficult bacteria, etc.

Active Publication Date: 2011-06-29
SHANGHAI JIAODA ONLLY CO LTD
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the 16S rRNA gene sequence is highly conserved, there is little difference in the 16S rRNA gene sequence between certain species of Bifidobacteria, so it is difficult to clearly distinguish bacteria into species (Rossello-Mora & Amman( 2001), Thespecies concept for prokaryotes.FEMS Microbiology Reviews, 25:39-67), we need to find a method that can conveniently and quickly identify Bifidobacterium strains to the species level

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for identifying bifidobactirium
  • Method for identifying bifidobactirium
  • Method for identifying bifidobactirium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] The identification of embodiment 1 Bifidobacterium lactis BL99

[0061] After the Bifidobacterium lactis strain BL99 was anaerobically cultured in the MRS liquid medium for 24 hours, 1ml of the bacterial liquid was taken to extract genomic DNA, and the DNA of the bacterial strain was used as a template, and the genus-specific primer pair Bif500F & Bif500R was used as primers for PCR amplification. Strain BL99 can be amplified to obtain a target fragment of about 500bp, indicating that the strain belongs to the genus Bifidobacterium; at the same time, primer pairs Bif900F & Bif900R and Pk300F & Pk00R were used for PCR amplification, and the electrophoresis of the amplified product is as follows figure 1 shown.

[0062] The obtained three target gene fragments were purified and sequenced respectively, and the following results were obtained:

[0063] Alignment results of target fragment 1 (Bif500 primer pair): the strains with the highest homology include Bifidobacterium...

Embodiment 2

[0067] Identification of Example 2 Bifidobacterium longum BL88-Onlly

[0068] After the Bifidobacterium longum strain BL88 was anaerobically cultured in the MRS liquid medium for 24 hours, 1ml of the bacterial liquid was taken to extract genomic DNA, and the DNA of the bacteria was used as a template, and the genus-specific primer pair Bif500F & Bif500R was used as primers for PCR amplification. The strain BL88-Onlly can be amplified to obtain a target fragment of about 500bp, indicating that the strain belongs to the genus Bifidobacterium. Carry out PCR amplification with primer pair Bif900F & Bif900R and Pk300F & Pk00R simultaneously, the electrophoresis figure of amplified product is as follows figure 1 shown.

[0069] The obtained three target gene fragments were purified and sequenced respectively, and the following results were obtained:

[0070] Alignment results of target fragment 1 (Bif500 primer pair): the strains with the highest homology include Bifidobacterium l...

Embodiment 3

[0074] Example 3 Identification of Bifidobacterium breve BB8

[0075] After the Bifidobacterium breve strain BB8 was anaerobically cultured in the MRS liquid medium for 24 hours, 1 ml of the bacterial liquid was taken to extract genomic DNA, and the DNA of the bacteria was used as a template, and the genus-specific primer pair Bif500F & Bif500R was used as primers for PCR amplification. Strain BB8 can be amplified to obtain a target fragment of about 500bp, indicating that the strain belongs to the genus Bifidobacterium. Simultaneously carry out PCR amplification with primer pair Bif900F & Bif900R and Pk300F & Pk00R, the electrophoresis figure of amplified product is as follows figure 1 shown.

[0076] The obtained three target gene fragments were purified and sequenced respectively, and the following results were obtained:

[0077] Alignment results of target fragment 1 (Bif500 primer pair): the most homologous strain of Bifidobacterium breve B.breve. The target fragment s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for identifying bifidobactirium, in particular to a novel molecular biology method for identifying bifidobactirium. According to the method, PCR (Polymerase Chain Reaction) amplification and sequencing analysis are performed on two target gene segments on bifidobactirium 16SrDNA and one target gene segment on pyruvate kinase coded gene, so that the bifidobactirium strain can be identified on a seed level. The invention provides a more accurate practical method which is much easier for operation for identifying bifidobactirium and is favorable for sorting and research works of the bifidobactirium strains.

Description

technical field [0001] The invention relates to bifidobacteria, in particular to a molecular biology method for identifying bifidobacteria. More precisely, the present invention provides a PCR (Polymerase Chain Reaction, polymerase chain reaction, referred to as PCR) amplification method for two target segments on the bifidobacterium 16S rRNA gene and a target segment on the pyruvate kinase gene. Augmentation and sequencing analysis, a method for the identification of bifidobacterial strains to the species level. Background technique [0002] Bifidobacterium is a Gram-positive bacterium, an obligate anaerobic bacterium with polymorphic characteristics, but overall it has a typical morphology of "branched". According to the description in "Bergey's Bacterial Identification Manual", the genus Bifidobacterium belongs to the class Actinobacteria, the order Bifidobacteriales, and the family Bifidobacteriaceae. In 1899, Dr. Tisser of the Institut Pasteur in France discovered Bif...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68
Inventor 张旻杭晓敏张和春陈培青
Owner SHANGHAI JIAODA ONLLY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com