Agent for induction/maintenance of remission
A technology of inhibitors and antagonists, which is applied in the field of remission and maintenance therapeutic agents for immune diseases, can solve the problems of non-disclosure and the like, and achieve the effect of alleviating immune diseases
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[0057] Rat Adjuvant Arthritis Model
[0058] 0.05 mL (0.6 mg / rat) of M. butyricum dead bacteria (12 mg / mL) suspended in liquid paraffin was injected intradermally into the dorsum of the right hind limb of 8-week-old LEW / CrlCrlj female rats (Charles-River, Japan). ), triggering arthritis (Day 0). The drug solution prepared with 0.5% methylcellulose (0.5% MC) was administered to the gastrointestinal tract at 0.5 mL / 100 g rat body weight. The adjuvant control was given only 0.5% MC. On days 0 to 10, methotrexate (MTX, Sigma) was given daily at 0.1 mg / kg once a day. Switch to (R)-2-amino-5-[2-chloro-4-(3-trifluoromethylphenylthio)phenyl]-2-methyl-1-pentanol hydrochloride from day 11 Salt (hereinafter referred to as Compound 1, 1 mg / kg) was administered once a day until the 24th day. The evaluation of arthritis was carried out as follows: Use a volumetric measuring instrument (MK-550, Muromachi Machinery) to measure the volume of the left and right hind limbs on the 0th, 8th,...
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