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Constructing method and application of nucleic acid library in MHC (Major Histocompatibility Complex) region

A nucleic acid library and region technology, which is used in the construction of nucleic acid libraries in the MHC region to achieve the effect of promoting the development process and application

Active Publication Date: 2014-08-27
BGI GENOMICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0014] Aiming at the defects of current MHC region research methods, the present invention provides a research strategy for MHC region by combining high-throughput sequencing technology and sequence capture technology, specifically the construction method and application of MHC region nucleic acid library

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  • Constructing method and application of nucleic acid library in MHC (Major Histocompatibility Complex) region
  • Constructing method and application of nucleic acid library in MHC (Major Histocompatibility Complex) region
  • Constructing method and application of nucleic acid library in MHC (Major Histocompatibility Complex) region

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Embodiment Construction

[0047] Embodiments of the present invention will be described in detail below in conjunction with examples, but those skilled in the art will understand that the following examples are only used to illustrate the present invention, and should not be considered as limiting the scope of the present invention. Those who do not indicate the specific conditions in the examples are carried out according to the conventional conditions or the conditions suggested by the manufacturer. The reagents or instruments used were not indicated by the manufacturer, and they were all commercially available conventional products. The manufacturer's article number of each reagent or kit is in brackets below.

[0048] The list of instruments used in the embodiment of the present invention part:

[0049]

[0050] List of main reagents

[0051]

[0052] The primer sequence information is as follows:

[0053]

[0054]

[0055] Step 1 Probe Design.

[0056] At the same time, select the ...

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Abstract

Provided are a method for constructing an MHC region nucleic acid library, an MHC region nucleic acid library, a method for sequencing the MHC region, a method for determining a type of gene mutation in the MHC region, and a use of the method for sequencing the MHC region in MHC genotyping. The method for constructing an MHC region nucleic acid library comprises the following steps: preparing a genomic library, wherein the genomic library comprises genomic DNA segments; and screening the genomic library by using a probe to obtain a target library, wherein the probe is specific for the MHC region, and the target library forms the MHC region nucleic acid library.

Description

technical field [0001] The present invention relates to a method for constructing a nucleic acid library, in particular to a method for constructing a target library, especially a nucleic acid library for the MHC region, and its application. Background technique [0002] Major Histocompatibility Complex (MHC) refers to a group of tightly linked gene groups on a chromosome of vertebrates that encode major histocompatibility antigens, control mutual recognition between cells, and regulate immune responses. The discovery became one of the most studied regions of the vertebrate genome after it was discovered in mice in 1936. [0003] Human major histocompatibility antigen was first discovered on the surface of leukocytes, so it is called human leukocyte antigen (human leucocyte antigen, HLA) [1] . The HLA complex is located at 6P21.31, the short arm of human chromosome 6, with a total length of about 4MB. From the centromere side, it is located in the regions of class II genes...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C40B50/00C40B40/08C12Q1/68
CPCC07K14/70539
Inventor 田埂蒋慧刘晓曹红志武靖华赵美茹吴仁花欧阳伟汉
Owner BGI GENOMICS CO LTD
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