Method for quickly propagating pistacia chinensis bunge by using stem sections
A technology of Pistacia chinensis and stem segments, which is applied in the field of rapid propagation of Pistacia chinensis stem segments, can solve the problems of large-scale, rapid, and at any time propagation of Pistacia chinensis seedlings, cannot meet the rapid development of the seedling Pistacia chinensis industry, and other problems, so as to ensure genetic stability. Sex, excellent germplasm preservation, and the effect of small variation in plant progeny
Active Publication Date: 2012-12-19
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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Problems solved by technology
The above reasons bring difficulties to large-scale, rapid and ready-to-propagate Pistacia seedlings, far from satisfying the demand for seedlings and the rapid development of Pistacia chinensis industry
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[0020] First, the stems of Pistacia chinensis were taken, soaked in 70% ethanol for 1 minute, and then treated with 0.1% HgCl 2 Soak for 10 minutes, shaking constantly. After disinfection, use sterile surgical scissors to cut it into axillary bud stem segments of about 1 cm, and then inoculate them on the bud induction medium. After about 25 days, 2-4 cm long shoots can be induced. The sprouts can be divided into many stem segments, and then induced into new shoots again, and the induction rate is over 88%; when the shoots grow stronger, they are transferred to the rooting medium, and the root tips can be seen in about 25 days After 10 days, each plant can grow 2-3 strong root systems on average, and then the complete plant with roots and new shoots will be transplanted to the field.
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The invention provides a method for quickly propagating pistacia chinensis bunge by using stem sections, which comprises: cutting off stem sections from pistacia chinensis bunge plants, disinfecting and inoculating onto a bud-inducing culture medium; transferring induced buds into a rooting culture medium to obtain whole plants; cutting induced tender shoots a plurality of stem sections; and repeatedly culturing in the same way to realize quick propagation. The offsprings of plants cultured by the method have few variations, and thus, a support is provided for the quick development of pistacia chinensis bunge ecological energy forests.
Description
technical field [0001] The invention relates to the technical field of biological propagation, in particular to a method for rapid propagation of pistachio chinensis stem segments. Background technique [0002] Pistacia is currently the most promising woody energy plant in my country. It is wild and cultivated in 24 provinces (autonomous regions and municipalities) south of Hebei in China. The oil content of its seeds is as high as 42%. Biodiesel produced from Pistacia oil It is a clean renewable energy source and a high-quality substitute for petroleum diesel. [0003] The pistachio seedling base basically breeds seedlings by seeds and then transplants them. Due to the large amount of secondary metabolites contained in Pistacia chinensis, cutting propagation is difficult. The germination rate of Pistacia chinensis propagating by seeds is low, below 40%. Moreover, the seeds are raw materials for extracting biodiesel, so using seeds to germinate is not only wasteful, bu...
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IPC IPC(8): A01H4/00
Inventor 吴丽芳李明浩安倩张萍萍
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI