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Tissue culture and rapid propagation method for high-value timber tree species albizia odoratissima

A technology for tissue culture rapid propagation and Albizia juniperus, which is applied in the field of plant tissue culture, can solve the problems of uneven seedlings, unstable genetic traits, and low reproductive efficiency, so as to promote seedling growth, shorten the subculture cycle, and increase proliferation. The effect of the coefficient

Active Publication Date: 2020-12-18
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved in the present invention is to provide a method for tissue culture and rapid propagation of high-value timber tree species Albizia julienne, which can improve the initial bud induction rate, cluster bud induction rate, rooting rate and shorten the subculture proliferation cycle to replace the existing technology The method of seed propagation is used in the medium to solve the problems of unstable genetic traits of seedlings, uneven seedlings, and low reproductive efficiency

Method used

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  • Tissue culture and rapid propagation method for high-value timber tree species albizia odoratissima
  • Tissue culture and rapid propagation method for high-value timber tree species albizia odoratissima
  • Tissue culture and rapid propagation method for high-value timber tree species albizia odoratissima

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Embodiment 1

[0037] A method for tissue culture and rapid propagation of high-value timber tree species Albizia juliensis, comprising the following steps:

[0038] A. Excellent plant selection: select the approved fine varieties of Albizia julienne or the straight stem, luxuriant growth, healthy and no pests and diseases in the natural community as the excellent single plant;

[0039] B. Selection and processing of explants: carry out pile cutting or ring cutting on the selected excellent plants, collect young shoots of the year, with axillary buds or terminal buds, and a length of 3 cm as explants after germination, and cut off most of them Rinse the leaves with clean water, soak them in dephosphorization washing powder for 5 minutes, then brush the explants with a soft brush, and rinse them with clean water for 0.5 hours; soak them in 75% alcohol for 10 seconds on an ultra-clean bench, and then use Rinse once with sterile water and put it in 0.1% HgCl 2 Soak in the solution for 15 minut...

Embodiment 2

[0050] A method for tissue culture and rapid propagation of high-value timber tree species Albizia juliensis, comprising the following steps:

[0051] A. Excellent plant selection: select the approved fine varieties of Albizia julienne or the straight stem, luxuriant growth, healthy and no pests and diseases in the natural community as the excellent single plant;

[0052] B. Selection and processing of explants: carry out stake cutting or circumcision on the selected excellent plants, collect young shoots of the year, with axillary buds or terminal buds, and a length of 4 cm as explants after germination, and cut off most of them Leaves were rinsed with clean water, then soaked in dephosphorization washing powder for 10 minutes, then brushed the explants with a soft brush, and then rinsed with clean water for 1 hour; soaked in 75% alcohol for 15 seconds on an ultra-clean bench, and then rinsed with non-toxic Rinse twice with bacterial water, put it into 0.1% HgCl 2 Soak in th...

Embodiment 3

[0063] A method for tissue culture and rapid propagation of high-value timber tree species Albizia juliensis, comprising the following steps:

[0064] A. Excellent plant selection: select the approved fine varieties of Albizia julienne or the straight stem, luxuriant growth, healthy and no pests and diseases in the natural community as the excellent single plant;

[0065] B. Selection and processing of explants: carry out pile cutting or circumcision on the selected excellent plants, collect young shoots of the year, with axillary buds or terminal buds, and a length of 6 cm as explants after germination, and cut off most of them Rinse the leaves with clean water, then soak them in dephosphorization washing powder for 15 minutes, then brush the explants with a soft brush, and then rinse them with clean water for 1.2 hours; soak them in 75% alcohol for 20 seconds on an ultra-clean bench, and then use Rinse 3 times with sterile water and put it in 0.1% HgCl 2 Soak in the solutio...

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Abstract

The invention discloses a tissue culture and rapid propagation method for high-value timber tree species albizia odoratissima. The method comprises the following steps of selecting excellent single plants, carrying out stump cutting or girdling, and collecting twigs as explants after germination; cleaning, sterilizing and disinfecting the explants, and then absorbing moisture by using sterilized absorbent paper; then carrying out primary induction to obtain initial buds; carrying out subculture multiplication culture on the initial buds to obtain subculture cluster buds; carrying out rooting culture on the subculture cluster buds to obtain tissue culture rooting seedlings; after tissue culture rooting seedlings are hardened, transplanting the tissue culture rooting seedlings into a disinfected seedling culture container, and finally transplanting the seedlings out of a nursery for afforestation. According to the method, a seed propagation mode adopted in the prior art is replaced, andthe problems that the hereditary characters of the seedlings are unstable, the seedlings are uneven and the propagation efficiency is not high are solved.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for tissue culture and rapid propagation of high-value timber tree species Albizia julienne. Background technique [0002] Albizia odoratissima (L.f.) Benth, also known as Niujiao Sen and Haig, is a tall deciduous tree of the genus Albizia Fabaceae. It is mainly distributed in Hainan, Sichuan, Guangdong, Guangxi, Guizhou, and Yunnan, mostly vertically distributed at altitudes The middle and lower parts of low mountains and hills below 800m are native tree species in Guangxi. Acacia is light-loving, slightly shade-tolerant, fast-growing, and the heartwood forms faster than Dalbergia balsamic and lattice wood. When young, the bark is light gray with lenticels, and when it grows up it turns dark brown and falls off in scales. The young branches of Albizia chrysalis are densely covered with downy hairs, and the downy hairs fall off when they grow u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001Y02P60/40Y02A40/22
Inventor 张烨韦铄星刘海龙李松海欧汉彪韦海航
Owner GUANGXI FORESTRY RES INST