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Method for preventing tissue-cultured thrips from being polluted

A technology for tissue culture and thrips, which is applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of affecting the economic benefits of enterprises, pollution, and high bacterial content in the air, so as to prevent thrips pollution and reduce production. cost, the effect of improving production efficiency

Inactive Publication Date: 2011-08-10
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the tissue culture industry has just started in my country, especially in Jiangsu and Zhejiang regions, where the climate is warm and humid, and the air contains high bacteria. Most of the tissue culture rooms are not isolated, and tiny insects such as ants, thrips, and spiders can easily enter the room and drill into the culture container. Cause a lot of pollution, the pollution rate of tissue culture seedlings often greatly exceeds 5%, some even exceed 50%, seriously affecting the economic benefits of enterprises

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A method for preventing the contamination of tissue-cultured thrips, carried out as follows:

[0017] 1) After conventional tissue culture inoculation, cover the culture container with a lid, or plug the culture container with a stopper; 2), apply or spray thripsicide on the place where the bottom of the cover contacts the culture container or where the mouth of the culture container contacts the plug; 3) Add a parafilm at the place where the bottom of the cover contacts the culture vessel or where the mouth of the culture vessel contacts the stopper.

[0018] The culture container includes a transparent plastic culture container, a glass culture bottle, a triangular flask, and a test tube.

[0019] The thripsicide includes 1000 times of 2.5% Caixi suspension concentrate, 1000 times of Yibaijing (10% imidacloprid wettable powder) or 2000 times of 20% Haoniandong EC.

[0020] Described sealing film comprises tissue culture special sealing film, Palafilm film, fresh-keep...

Embodiment 2

[0023] In this example, 1), after conventional tissue culture inoculation, cover the culture container; 2), spray thripsicide on the place where the bottom of the cover is in contact with the culture container; 3), seal the place where the bottom of the cover is in contact with the culture container membrane.

[0024] The culture container includes a transparent plastic culture container and a glass culture bottle.

[0025] The described thripsicide is 1000 times of 2.5% Caixi suspension concentrate.

[0026] Described sealing film is fresh-keeping film.

[0027] The thripsicide contamination of tissue culture includes Thrips palmi Karny, Trips flavus Schrank, and fungi, bacteria and other contamination caused by thrips contamination.

Embodiment 3

[0029] In this example, 1), plug the culture container after conventional tissue culture inoculation; 2), apply thripsicide at the contact between the culture container mouth and the plug; 3), seal the sealing film at the contact between the culture container mouth and the plug .

[0030] The culture container includes a transparent Erlenmeyer flask and a test tube.

[0031] The thripsicide is 1000 times that of Yiyijing (10% imidacloprid wettable powder).

[0032] The parafilm is a special parafilm and Palafilm for tissue culture.

[0033] The thrips contamination in tissue culture includes Thrips palmi Karny, Trips flavus Schrank, and fungi, bacteria and other contamination caused by thrips contamination.

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Abstract

The invention discloses a method for preventing tissue-cultured thrips from being polluted. The method comprises the following steps of: 1) covering a cover on a culture vessel after the conventional tissue culture and inoculation, or plugging a stopper into the culture vessel; 2) spreading or spraying a thrips killing agent at the contact position of the lower edge of the cover and the culture vessel or at the contact position of the mouth of the culture vessel and the stopper; and 3) sealing a sealing film at the contact position of the lower edge of the cover and the culture vessel or at the contact position of the mouth of the culture vessel and the stopper. The invention has the advantages that: by the method, the pollution of plant tissue-cultured thrips and the pollution of fungi, bacteria and the like caused by the pollution of the thrips can be effectively prevented, the production efficiency can be greatly improved, and the production cost can be reduced; and the method is favorable for large-scale industrial production.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture rapid propagation, in particular to a method for preventing thrips from tissue culture pollution. Background technique [0002] Plant tissue culture technology is one of the most successful biotechnology applications in the world in recent decades. Obvious economic, social and ecological benefits have been achieved in the rapid propagation of clones, the cultivation of virus-free seedlings, the accelerated breeding process, the selection of fine varieties and the low-temperature preservation of germplasm resources. role is also becoming more and more important. However, in the industrial production process of tissue culture rapid propagation, the main problem is the frequent occurrence of tissue culture pollution. Tissue culture pollution not only seriously affects the quality and yield of tissue culture seedlings, but also greatly increases production costs. It is reported that ev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01N51/00A01N47/22A01N43/22A01P7/04
Inventor 汪一婷王汉荣陈剑平王连平徐刚吕永平牟豪杰
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES