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Preparation method of medicine for preventing and curing poultry viral disease and application of medicine

A poultry viral disease and drug technology, applied in the field of medicine, can solve the problems of high cost and unsuitable for promotion and use in the breeding industry, and achieve the effects of low cost, improved animal immunity, and high extraction rate

Inactive Publication Date: 2013-01-02
TIANJIN ASIAN BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The PHA currently used in the market is all refined and purified, and the cost is relatively high, so it is not suitable for promotion and use in the aquaculture industry.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A kind of preparation method of red kidney bean extract, concrete implementation steps are as follows:

[0030] (1) Select 100g of red kidney beans, rinse with distilled water, soak in 75% alcohol for 10 minutes, pour off the alcohol, and place it in a 37°C thermostat for 24 hours;

[0031] (2) Rinse twice with sterile normal saline under aseptic conditions, add 100 ml of normal saline containing 0.1% β-mercaptoethanol, and grind the red kidney beans;

[0032] (3) Add 400ml of normal saline to soak overnight, and centrifuge at 3000-4000rpm for 30 minutes;

[0033] (4) Add solid ammonium sulfate to the supernatant to reach 80% saturation, and place it overnight;

[0034] (5) Centrifuge at 3000-4000rpm for 30 minutes, discard the supernatant, add 100ml of distilled water to the sediment, and decolorize it with activated carbon;

[0035] (6) Sterilize by filtration and dry in vacuum.

Embodiment 2

[0037] A kind of preparation method of red kidney bean extract, concrete implementation steps are as follows:

[0038] (1) Select 50g of red kidney beans, soak them in 75% alcohol for 15 minutes, pour off the alcohol, and place them at room temperature for 16 hours;

[0039] (2) Rinse twice with sterile normal saline, add 100ml of normal saline containing 0.05% β-mercaptoethanol, and grind red kidney beans with a tissue masher;

[0040] (3) Add 250ml of normal saline to soak overnight, and centrifuge at 3000-4000rpm for 30 minutes;

[0041] (4) Add solid ammonium sulfate to the supernatant to reach 60% saturation, and leave it for 24 hours;

[0042] (5) Centrifuge at 3000-4000rpm for 30 minutes, add 80ml of PBS to the sediment, and decolorize it with activated carbon;

[0043] (6) Sterilize by filtration, pack in separate packages and store in the refrigerator.

[0044] (7) Drying: the red kidney bean extract was freeze-dried.

Embodiment 1

[0046] Therapeutic Effect of Red Kidney Bean Extract on Chicken Marek's Disease

[0047]Treatment test in the artificial pathogenic virus model 54 SPF chickens were artificially infected with chicken Marek virus to make chicks develop chicken Marek, and they were randomly divided into 3 groups, 18 in each group. Group A is the "red kidney bean extract" treatment group, and the mass percentage of the red kidney bean extract is 30%; group B is the astragalus polysaccharide treatment group, and the mass percentage of astragalus polysaccharide is 30%; group C is the control Group A was treated with normal saline, and the three groups of chickens were infected with chicken Marek virus at the same time, and each chicken was injected with chicken Marek virus strong poison 0.2ml. After clinical symptoms appeared, the chickens of group A were injected with "red kidney bean extract". "(use dosage is 0.5 / Kg body weight), once a day, B group chicken injection stilbene polysaccharide (use ...

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PUM

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Abstract

The invention provides a preparation method of a medicine for preventing and curing the poultry viral disease and the application of the medicine, belongs to the field of medicine, and in particular relates to the preparation method of the medicine for preventing and curing the poultry viral disease and the application of the medicine. The preparation method of the medicine comprises the following steps of: soaking red kidney bean by 75% ethyl alcohol, extracting by adding belt-mercaptoethanol-containing physiological saline, and levigating the red kidney bean; soaking for one night by addingthe physiological saline, and precipitating by adding solid ammonium sulfate into supernate after centrifuging; and dissolving by adding distilled water into precipitate, decoloring, degerming in a filtering way, and drying to obtain the medicine. The red kidney bean extract is used for preventing and curing the poultry viral disease, so that the medicine has not only the antiviral function and but also the immunity-adjusting function, is exact in curative effects, and has no toxicant and side effects. The invention particularly has the obvious effects for curing the fowl paralysis, the atypical newcastle disease, the epidemical bronchitis and the like.

Description

technical field [0001] The invention relates to the field of medicine, and more specifically, relates to a preparation method of a medicine for preventing and treating avian viral diseases and an application of the product. Background technique [0002] With the development of the society, the prosperity of the economy and the change of the concept of human health, the concept of returning to nature and the unity of man and nature has been more and more respected, "table safety" has received great attention, and people's requirements for food safety getting higher and higher. Among poultry diseases, virus infection causes the greatest loss to the poultry farming industry. Chicken infectious bursal disease, chicken Newcastle disease, and chicken infectious bronchitis are the three major diseases that endanger the poultry industry. In some areas where chickens are densely raised, they have replaced atypical Newcastle disease and ranked first in the incidence of infectious dis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/48A61P31/12A61P31/14A61K131/00
Inventor 王文彪王丽莉
Owner TIANJIN ASIAN BIOENG
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