Simple culture method for effective microorganisms (EM)
A culture method, EM bacteria technology, applied in the field of mixed bacteria culture, to achieve low investment, avoid a lot of loss, easy to carry and use
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Embodiment 1
[0020] 1.1 Configure culture medium
[0021] Sterilize and disinfect 20L plastic square barrels and measuring cylinders with ventilated lids.
[0022] Weigh 6% molasses water and 0.5% peptone respectively by weight, add purified water, and configure a total of 20 kg of culture solution.
[0023] 1.2 Inoculation of Bacillus
[0024] Weigh 1 kg of bacillus (purchased from Changan Biological Co., Ltd.), and put the weighed bacteria into the culture solution.
[0025] 1.3 Incubate the inoculated transparent plastic bucket at 20-35°C for 24 hours.
[0026] 1.4 Inoculation of compound lactic acid bacteria, yeast and photosynthetic bacteria
[0027] Weigh 1.2kg of compound lactic acid bacteria (purchased from Guangming Dairy Factory), 1.0kg of saccharomyces (purchased from Angel Yeast Co., Ltd.), and 0.8kg of photosynthetic bacteria (purchased from Changan Biological Co., Ltd.), and put the weighed strains into into the culture medium.
[0028] 1.5 Cultivate the inoculated trans...
Embodiment 2
[0030] 2.1 Configure culture medium
[0031] The 25L plastic square barrels and measuring cylinders with ventilated lids were sterilized.
[0032] Weigh 6% molasses water and 0.5% peptone respectively by weight, add purified water, and configure a total of 25 kg of culture solution.
[0033] 2.2 Inoculation of Bacillus
[0034] Weigh 1.25 kg of bacillus (purchased from Changan Biological Co., Ltd.), and put the weighed strains into the culture solution.
[0035] 2.3 Incubate the inoculated transparent plastic bucket at 25-35°C for 24 hours.
[0036] 2.4 Inoculation of compound lactic acid bacteria, yeast and photosynthetic bacteria
[0037] Weigh 1.5kg of compound lactic acid bacteria (purchased from Guangming Dairy Factory), 1.25kg of saccharomyces (purchased from Angel Yeast Co., Ltd.), and 1.0kg of photosynthetic bacteria (purchased from Changan Biological Co., Ltd.), and put the weighed strains into into the culture medium.
[0038] 2.5 Cultivate the inoculated transp...
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